(Circular) (Six-base) MAPSORT of: pFN21AB8330.seq Check: 1433 from: 1 to: 5116 pFN21AB8330 5116 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2926 278 Size: 53 83 186 936 1390 2468 Fragments arranged by size: 2468 1390 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2459 1937 Size: 522 4594 Acc65I G'GTAC_C Cuts at: 2437 2437 Size: 5116 AclI AA'CG_TT Cuts at: 1563 3243 3616 1563 Size: 1680 373 3063 Fragments arranged by size: 3063 1680 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3179 4563 4831 1632 Size: 1547 1384 268 1917 Fragments arranged by size: 1917 1547 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4919 AflIII A'CryG_T Cuts at: 1203 4015 4992 1203 Size: 2812 977 1327 Fragments arranged by size: 2812 1327 977 AgeI A'CCGG_T Cuts at: 3947 3947 Size: 5116 AhdI GACnn_n'nnGTC Cuts at: 3845 3845 Size: 5116 AleI CACnn'nnGTG Cuts at: 1166 2198 1166 Size: 1032 4084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5116 AlwNI CAG_nnn'CTG Cuts at: 2859 4431 2859 Size: 1572 3544 ApaI G_GGCC'C Cuts at: 2235 2235 Size: 5116 ApaLI G'TGCA_C Cuts at: 3173 4329 3173 Size: 1156 3960 ApoI r'AATT_y Cuts at: 1493 2240 2425 2651 2803 4912 1493 Size: 747 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 747 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 3670 160 Size: 872 2638 1606 Fragments arranged by size: 2638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5116 AvaI C'yCGr_G Cuts at: 1870 1943 2441 1870 Size: 73 498 4545 Fragments arranged by size: 4545 498 73 BamHI G'GATC_C Cuts at: 2446 2813 2446 Size: 367 4749 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2034 2437 3892 618 Size: 325 217 627 247 403 1455 1842 Fragments arranged by size: 1842 1455 627 403 325 247 217 BanII G_rGCy'C Cuts at: 1391 2235 2435 1391 Size: 844 200 4072 Fragments arranged by size: 4072 844 200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3307 3341 1903 Size: 34 1370 34 3678 Fragments arranged by size: 3678 1370 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3008 4218 3008 Size: 1210 3906 BclI T'GATC_A Cuts at: 1548 1548 Size: 5116 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3727 136 Size: 107 122 71 631 605 2055 1525 Fragments arranged by size: 2055 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5111 5111 Size: 5116 BlpI GC'TnA_GC Cuts at: 2533 2533 Size: 5116 Bme1580I G_kGCm'C Cuts at: 1790 2235 3177 4333 1790 Size: 445 942 1156 2573 Fragments arranged by size: 2573 1156 942 445 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2847 3805 442 Size: 416 53 83 399 24 1430 958 1753 Fragments arranged by size: 1753 1430 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 3776 1239 Size: 1163 1374 2579 Fragments arranged by size: 2579 1374 1163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3241 4106 4404 3241 Size: 865 298 3953 Fragments arranged by size: 3953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2072 3779 882 Size: 617 573 1707 2219 Fragments arranged by size: 2219 1707 617 573 BsaAI yAC'GTr Cuts at: 493 1204 2907 493 Size: 711 1703 2702 Fragments arranged by size: 2702 1703 711 BsaBI GATnn'nnATC Cuts at: 5110 5110 Size: 5116 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2923 3305 3940 275 Size: 53 83 186 936 1390 382 635 1451 Fragments arranged by size: 1451 1390 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2184 3549 3947 4221 4368 4779 2184 Size: 1365 398 274 147 411 2521 Fragments arranged by size: 2521 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2110 2140 1178 Size: 30 165 30 707 30 4154 Fragments arranged by size: 4154 707 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5116 BseYI C'CCAG_C Cuts at: 2053 4319 4815 2053 Size: 2266 496 2354 Fragments arranged by size: 2354 2266 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 2372 1163 Size: 573 337 299 3907 Fragments arranged by size: 3907 573 337 299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2566 3327 4355 1540 Size: 275 186 565 761 1028 2301 Fragments arranged by size: 2301 1028 761 565 275 186 BsiHKAI G_wGCw'C Cuts at: 3177 3262 4333 3177 Size: 85 1071 3960 Fragments arranged by size: 3960 1071 85 BsmI GAATG_Cn' Cuts at: 2627 2720 2627 Size: 93 5023 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2435 3177 3262 4333 1391 Size: 399 445 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 445 399 200 85 BspEI T'CCGG_A Cuts at: 4779 4779 Size: 5116 BspHI T'CATG_A Cuts at: 3005 4735 3005 Size: 1730 3386 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2471 844 Size: 1627 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3003 1394 Size: 429 1180 3507 Fragments arranged by size: 3507 1180 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3611 3785 65 Size: 1183 2363 174 1396 Fragments arranged by size: 2363 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3760 3947 1700 Size: 110 1950 187 2869 Fragments arranged by size: 2869 1950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5116 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5116 BssSI C'ACGA_G Cuts at: 2390 3176 4188 2390 Size: 786 1012 3318 Fragments arranged by size: 3318 1012 786 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5116 BstBI TT'CG_AA Cuts at: 3920 3920 Size: 5116 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5116 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2200 2161 Size: 39 5077 BstYI r'GATC_y Cuts at: 1972 2446 2813 3198 3215 4667 4753 4765 5111 Size: 474 367 385 17 1452 86 12 346 Cuts at: 5111 1972 Size: 1977 Fragments arranged by size: 1977 1452 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2131 513 Size: 552 1066 3498 Fragments arranged by size: 3498 1066 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4377 Fragments arranged by size: 4377 683 56 BtsI GCAGTG_nn' Cuts at: 2632 3426 3446 2632 Size: 794 20 4302 Fragments arranged by size: 4302 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5081 DraI TTT'AAA Cuts at: 2420 2420 Size: 5116 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2348 4123 809 Size: 507 1032 1775 1802 Fragments arranged by size: 1802 1775 1032 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2563 3452 Size: 54 1425 50 275 18 372 361 889 Cuts at: 3452 5081 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 372 361 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2563 1537 Size: 275 751 4090 Fragments arranged by size: 4090 751 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3046 1353 Size: 1693 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3703 4077 4223 387 Size: 1064 138 2114 374 146 1280 Fragments arranged by size: 2114 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 3179 3776 4563 4831 1239 Size: 393 770 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 770 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2210 2231 2232 2210 Size: 21 1 5094 Fragments arranged by size: 5094 21 1 EcoRI G'AATT_C Cuts at: 2425 2803 2425 Size: 378 4738 FspI TGC'GCA Cuts at: 3622 3622 Size: 5116 HaeII r_GCGC'y Cuts at: 1678 4263 1678 Size: 2585 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 3757 3789 3831 Size: 32 135 32 599 32 1554 32 42 Cuts at: 3831 3863 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2460 2706 669 Size: 988 281 522 246 3079 Fragments arranged by size: 3079 988 522 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5116 HpaI GTT'AAC Cuts at: 2706 2706 Size: 5116 KpnI G_GTAC'C Cuts at: 2441 2441 Size: 5116 MfeI C'AATT_G Cuts at: 2715 2715 Size: 5116 MluI A'CGCG_T Cuts at: 4992 4992 Size: 5116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4230 4414 1305 Size: 2925 184 2007 Fragments arranged by size: 2925 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3294 Fragments arranged by size: 3294 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 3074 3433 3592 518 Size: 648 1032 876 359 159 2042 Fragments arranged by size: 2042 1032 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 2532 3209 4357 4602 1606 Size: 527 399 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 527 399 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4564 NdeI CA'TA_TG Cuts at: 387 387 Size: 5116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5116 NotI GC'GGCC_GC Cuts at: 2563 2563 Size: 5116 NspI r_CATG'y Cuts at: 1132 1229 2474 4019 1132 Size: 97 1245 1545 2229 Fragments arranged by size: 2229 1545 1245 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5116 PciI A'CATG_T Cuts at: 4015 4015 Size: 5116 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5116 PfoI T'CCnGG_A Cuts at: 2114 2114 Size: 5116 PmeI GTTT'AAAC Cuts at: 2420 2420 Size: 5116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3185 3217 4698 4730 3185 Size: 32 1481 32 3571 Fragments arranged by size: 3571 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2210 2210 Size: 5116 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5116 PsiI TTA'TAA Cuts at: 2686 2686 Size: 5116 PspOMI G'GGCC_C Cuts at: 2231 2231 Size: 5116 PstI C_TGCA'G Cuts at: 830 2468 830 Size: 1638 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5116 SacII CC_GC'GG Cuts at: 2134 2134 Size: 5116 SalI G'TCGA_C Cuts at: 1936 2458 1936 Size: 522 4594 SbfI CC_TGCA'GG Cuts at: 2468 2468 Size: 5116 ScaI AGT'ACT Cuts at: 3364 5076 3364 Size: 1712 3404 SfcI C'TryA_G Cuts at: 826 1045 2464 3599 4280 4471 826 Size: 219 1419 1135 681 191 1471 Fragments arranged by size: 1471 1419 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5116 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5116 SmaI CCC'GGG Cuts at: 1872 2443 1872 Size: 571 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3220 4121 4383 820 Size: 197 926 1277 901 262 1553 Fragments arranged by size: 1553 1277 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2907 493 Size: 2414 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5116 SphI G_CATG'C Cuts at: 1132 2474 1132 Size: 1342 3774 SspI AAT'ATT Cuts at: 5 52 3040 5 Size: 47 2988 2081 Fragments arranged by size: 2988 2081 47 StuI AGG'CCT Cuts at: 1517 2331 2369 1517 Size: 814 38 4264 Fragments arranged by size: 4264 814 38 StyI C'CwwG_G Cuts at: 513 1065 2122 2172 2360 2555 513 Size: 552 1057 50 188 195 3074 Fragments arranged by size: 3074 1057 552 195 188 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3143 3160 3313 3498 3943 325 Size: 1255 1563 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3362 5074 96 Size: 274 80 33 51 1443 1385 1712 138 Fragments arranged by size: 1712 1443 1385 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5076 5108 5076 Size: 32 5084 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5116 XbaI T'CTAG_A Cuts at: 2452 2452 Size: 5116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5116 XmaI C'CCGG_G Cuts at: 1870 2441 1870 Size: 571 4545 XmnI GAAnn'nnTTC Cuts at: 3245 3245 Size: 5116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2924 276 Size: 53 83 186 936 1390 2468 Fragments arranged by size: 2468 1390 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI