(Linear) (Six-base) MAPSORT of: KIBB8330.seq  Check: 385  from: 1  to: 411



KIBB8330.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AleI CACnn'nnGTG

Cuts at:      0     193     411 
   Size:        193     218
 
ApaI G_GGCC'C

Cuts at:      0     230     411 
   Size:        230     181
 
ApoI r'AATT_y

Cuts at:      0     235     411 
   Size:        235     176
 
BanI G'GyrC_C

Cuts at:      0      29     411 
   Size:         29     382
 
BanII G_rGCy'C

Cuts at:      0     230     411 
   Size:        230     181
 
Bme1580I G_kGCm'C

Cuts at:      0     230     411 
   Size:        230     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     411 
   Size:        397      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      67     411 
   Size:         67     344
 
BsaWI w'CCGG_w

Cuts at:      0     179     411 
   Size:        179     232
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     105     135     411 
   Size:        105      30     276

  Fragments arranged by size: 

                 276     105      30
 
BseYI C'CCAG_C

Cuts at:      0      48     411 
   Size:         48     363
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     367     411 
   Size:         68     299      44

  Fragments arranged by size: 

                 299      68      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     411 
   Size:        230     181
 
BssSI C'ACGA_G

Cuts at:      0     385     411 
   Size:        385      26
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     156     195     411 
   Size:        156      39     216

  Fragments arranged by size: 

                 216     156      39
 
BtgI C'CryG_G

Cuts at:      0     126     411 
   Size:        126     285
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     343     411 
   Size:        343      68
 
EaeI y'GGCC_r

Cuts at:      0     197     411 
   Size:        197     214
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     411 
   Size:        397      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     205     226     227     411 
   Size:        205      21       1     184

  Fragments arranged by size: 

                 205     184      21       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     411 
   Size:        166      32     213

  Fragments arranged by size: 

                 213     166      32
 
MslI CAynn'nnrTG

Cuts at:      0     193     411 
   Size:        193     218
 
MspA1I CmG'CkG

Cuts at:      0     128     411 
   Size:        128     283
 
PfoI T'CCnGG_A

Cuts at:      0     109     411 
   Size:        109     302
 
PpuMI rG'GwC_Cy

Cuts at:      0     205     411 
   Size:        205     206
 
PspOMI G'GGCC_C

Cuts at:      0     226     411 
   Size:        226     185
 
SacII CC_GC'GG

Cuts at:      0     129     411 
   Size:        129     282
 
StuI AGG'CCT

Cuts at:      0     326     364     411 
   Size:        326      38      47

  Fragments arranged by size: 

                 326      47      38
 
StyI C'CwwG_G

Cuts at:      0     117     167     355     411 
   Size:        117      50     188      56

  Fragments arranged by size: 

                 188     117      56      50
 

 Enzymes that do cut:

     AleI     ApaI     ApoI     BanI    BanII Bme1580I     BpmI     BsaI
    BsaWI    BsaXI    BseYI     BsgI Bsp1286I    BssSI    BstXI     BtgI
     DrdI     EaeI  Eco57MI EcoO109I    Hin4I     MslI   MspA1I     PfoI
    PpuMI   PspOMI    SacII     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI