(Circular) (Six-base) MAPSORT of: pFN21AE0350.seq Check: 5669 from: 1 to: 6946 pFN21AE0350 6946 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4756 278 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3944 4289 1937 Size: 2007 345 4594 Fragments arranged by size: 4594 2007 345 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 2106 2816 3902 5009 6393 6661 1632 Size: 417 57 710 1086 1107 1384 268 1917 Fragments arranged by size: 1917 1384 1107 1086 710 417 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 3567 3645 5845 6822 1203 Size: 2364 78 2200 977 1327 Fragments arranged by size: 2364 2200 1327 977 78 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 5675 5675 Size: 6946 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2944 1184 Size: 1760 5186 AlwNI CAG_nnn'CTG Cuts at: 4689 6261 4689 Size: 1572 5374 ApaLI G'TGCA_C Cuts at: 5003 6159 5003 Size: 1156 5790 ApoI r'AATT_y Cuts at: 1493 2737 3412 3793 3840 4077 4255 4481 4633 Size: 1244 675 381 47 237 178 226 152 Cuts at: 4633 6742 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1244 675 381 237 226 178 152 47 AseI AT'TA_AT Cuts at: 160 1032 3198 5500 160 Size: 872 2166 2302 1606 Fragments arranged by size: 2302 2166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 4271 1870 Size: 73 2328 4545 Fragments arranged by size: 4545 2328 73 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2790 4267 5722 618 Size: 325 217 627 1003 1477 1455 1842 Fragments arranged by size: 1842 1477 1455 1003 627 325 217 BanII G_rGCy'C Cuts at: 1391 3276 3739 3984 4265 1391 Size: 1885 463 245 281 4072 Fragments arranged by size: 4072 1885 463 281 245 BbeI G_GCGC'C Cuts at: 2794 2794 Size: 6946 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2249 3188 3499 4009 928 Size: 971 350 939 311 510 3865 Fragments arranged by size: 3865 971 939 510 350 311 BbvCI CC'TCA_GC Cuts at: 4224 4224 Size: 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5137 5171 1903 Size: 34 50 34 3116 34 3678 Fragments arranged by size: 3678 3116 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4838 6048 4838 Size: 1210 5736 BclI T'GATC_A Cuts at: 1548 1548 Size: 6946 BfrBI ATG'CAT Cuts at: 2439 3055 2439 Size: 616 6330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5557 136 Size: 107 122 71 631 605 3885 1525 Fragments arranged by size: 3885 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2139 6941 2139 Size: 4802 2144 BlpI GC'TnA_GC Cuts at: 3270 4025 4363 3270 Size: 755 338 5853 Fragments arranged by size: 5853 755 338 Bme1580I G_kGCm'C Cuts at: 1790 2434 5007 6163 1790 Size: 644 2573 1156 2573 Fragments arranged by size: 2573 2573 1156 644 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4677 5635 442 Size: 416 53 83 399 24 3260 958 1753 Fragments arranged by size: 3260 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 3461 3753 5606 1239 Size: 968 1254 292 1853 2579 Fragments arranged by size: 2579 1853 1254 968 292 Bpu10I CC'TnA_GC Cuts at: 2691 4224 2691 Size: 1533 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2745 3497 4142 5071 5936 6234 2745 Size: 752 645 929 865 298 3457 Fragments arranged by size: 3457 929 865 752 645 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 882 Size: 617 4110 2219 Fragments arranged by size: 4110 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2444 4737 493 Size: 711 1240 2293 2702 Fragments arranged by size: 2702 2293 1240 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2791 3100 4753 5135 Size: 53 83 186 936 1258 309 1653 382 Cuts at: 5135 5770 275 Size: 635 1451 Fragments arranged by size: 1653 1451 1258 936 635 382 309 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 5379 5777 6051 6198 6609 4149 Size: 1230 398 274 147 411 4486 Fragments arranged by size: 4486 1230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 3242 3272 3557 Size: 30 165 30 777 30 1032 30 285 Cuts at: 3557 3587 1178 Size: 30 4537 Fragments arranged by size: 4537 1032 777 285 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2550 2797 3313 3565 4103 1186 Size: 1364 247 516 252 538 4029 Fragments arranged by size: 4029 1364 538 516 252 247 BseYI C'CCAG_C Cuts at: 2023 6149 6645 2023 Size: 4126 496 2324 Fragments arranged by size: 4126 2324 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 3993 1163 Size: 573 642 1615 4116 Fragments arranged by size: 4116 1615 642 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 4031 5007 5092 6163 3669 Size: 362 976 85 1071 4452 Fragments arranged by size: 4452 1071 976 362 85 BsmI GAATG_Cn' Cuts at: 3758 4457 4550 3758 Size: 699 93 6154 Fragments arranged by size: 6154 699 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2434 3276 3669 3739 3984 4031 4265 Size: 399 644 842 393 70 245 47 234 Cuts at: 4265 5007 5092 6163 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 842 742 644 399 393 245 234 85 70 47 BspEI T'CCGG_A Cuts at: 4149 6609 4149 Size: 2460 4486 BspHI T'CATG_A Cuts at: 2158 3750 3762 4084 4835 6565 2158 Size: 1592 12 322 751 1730 2539 Fragments arranged by size: 2539 1730 1592 751 322 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4301 844 Size: 3457 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2058 5441 5615 65 Size: 1183 810 3383 174 1396 Fragments arranged by size: 3383 1396 1183 810 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2787 5590 5777 1700 Size: 110 977 2803 187 2869 Fragments arranged by size: 2869 2803 977 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 3351 794 Size: 2557 4389 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstYI r'GATC_y Cuts at: 1972 2139 3387 4276 4643 5028 5045 6497 6583 Size: 167 1248 889 367 385 17 1452 86 Cuts at: 6583 6595 6941 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1248 889 385 367 346 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6394 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3832 487 Size: 683 56 2606 3601 Fragments arranged by size: 3601 2606 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4076 4462 5256 5276 3349 Size: 727 386 794 20 5019 Fragments arranged by size: 5019 794 727 386 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3541 3576 529 Size: 35 2977 35 3899 Fragments arranged by size: 3899 2977 35 35 DraI TTT'AAA Cuts at: 3798 4250 3798 Size: 452 6494 DraIII CAC_nnn'GTG Cuts at: 2270 2270 Size: 6946 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2993 5953 809 Size: 507 1677 2960 1802 Fragments arranged by size: 2960 1802 1677 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3292 3562 4393 Size: 54 1425 50 275 18 1462 270 831 Cuts at: 4393 5282 6911 8 Size: 889 1629 43 Fragments arranged by size: 1629 1462 1425 889 831 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 4876 1353 Size: 672 2851 3423 Fragments arranged by size: 3423 2851 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5533 5907 6053 387 Size: 1064 138 3944 374 146 1280 Fragments arranged by size: 3944 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2106 2207 2816 3461 3753 3902 Size: 393 417 57 101 609 645 292 149 Cuts at: 3902 5009 5606 6393 6661 1239 Size: 1107 597 787 268 1524 Fragments arranged by size: 1524 1107 787 645 609 597 417 393 292 268 149 101 57 EcoNI CCTnn'n_nnAGG Cuts at: 3248 3680 3248 Size: 432 6514 EcoO109I rG'GnC_Cy Cuts at: 2581 2765 3169 4130 2581 Size: 184 404 961 5397 Fragments arranged by size: 5397 961 404 184 EcoRI G'AATT_C Cuts at: 4255 4633 4255 Size: 378 6568 EcoRV GAT'ATC Cuts at: 3778 4147 3778 Size: 369 6577 FspI TGC'GCA Cuts at: 2057 5452 2057 Size: 3395 3551 HaeII r_GCGC'y Cuts at: 1678 2794 6093 1678 Size: 1116 3299 2531 Fragments arranged by size: 3299 2531 1116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 2180 2212 2363 Size: 32 135 32 568 32 8 32 151 Cuts at: 2363 2395 2530 2562 3533 3565 3764 3796 5587 Size: 32 135 32 971 32 199 32 1791 Cuts at: 5587 5619 5661 5693 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1791 971 568 199 151 135 135 42 32 32 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 4290 4536 669 Size: 988 281 2352 246 3079 Fragments arranged by size: 3079 2352 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6946 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KasI G'GCGC_C Cuts at: 2790 2790 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2348 3736 6060 6244 1305 Size: 1043 1388 2324 184 2007 Fragments arranged by size: 2324 2007 1388 1043 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3564 10 Size: 54 1425 343 1732 3392 Fragments arranged by size: 3392 1732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2916 3060 3458 3662 3851 4904 Size: 648 1135 615 144 398 204 189 1053 Cuts at: 4904 5263 5422 518 Size: 359 159 2042 Fragments arranged by size: 2042 1135 1053 648 615 398 359 204 189 159 144 MspA1I CmG'CkG Cuts at: 1606 2027 4362 5039 6187 6432 1606 Size: 421 2335 677 1148 245 2120 Fragments arranged by size: 2335 2120 1148 677 421 245 NarI GG'CG_CC Cuts at: 2791 2791 Size: 6946 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6394 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NsiI A_TGCA'T Cuts at: 2441 3057 2441 Size: 616 6330 NspI r_CATG'y Cuts at: 1132 1229 2458 3269 3571 3649 4304 5849 1132 Size: 97 1229 811 302 78 655 1545 2229 Fragments arranged by size: 2229 1545 1229 811 655 302 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6946 PciI A'CATG_T Cuts at: 3567 3645 5845 3567 Size: 78 2200 4668 Fragments arranged by size: 4668 2200 78 PflMI CCAn_nnn'nTGG Cuts at: 1108 3428 1108 Size: 2320 4626 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2581 2765 3169 2581 Size: 184 404 6358 Fragments arranged by size: 6358 404 184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 2889 4516 2889 Size: 1627 5319 PstI C_TGCA'G Cuts at: 830 2897 4298 830 Size: 2067 1401 3478 Fragments arranged by size: 3478 2067 1401 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 2027 2027 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SbfI CC_TGCA'GG Cuts at: 4298 4298 Size: 6946 ScaI AGT'ACT Cuts at: 3821 5194 6906 3821 Size: 1373 1712 3861 Fragments arranged by size: 3861 1712 1373 SexAI A'CCwGG_T Cuts at: 3243 4196 3243 Size: 953 5993 SfcI C'TryA_G Cuts at: 826 1045 2893 2998 3147 3815 3945 4294 5429 Size: 219 1848 105 149 668 130 349 1135 Cuts at: 5429 6110 6301 826 Size: 681 191 1471 Fragments arranged by size: 1848 1471 1135 681 668 349 219 191 149 130 105 SfoI GGC'GCC Cuts at: 2792 2792 Size: 6946 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 2787 1700 Size: 1087 5859 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2760 3512 4121 5050 5951 6213 Size: 197 926 817 752 609 929 901 262 Cuts at: 6213 820 Size: 1553 Fragments arranged by size: 1553 929 926 901 817 752 609 262 197 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 3269 4304 1132 Size: 2137 1035 3774 Fragments arranged by size: 3774 2137 1035 SspI AAT'ATT Cuts at: 5 52 3209 3448 4870 5 Size: 47 3157 239 1422 2081 Fragments arranged by size: 3157 2081 1422 239 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6946 StyI C'CwwG_G Cuts at: 513 1065 2233 3975 4385 513 Size: 552 1168 1742 410 3074 Fragments arranged by size: 3074 1742 1168 552 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2924 3758 4973 4990 5143 5328 5773 Size: 1255 1344 834 1215 17 153 185 445 Cuts at: 5773 325 Size: 1498 Fragments arranged by size: 1498 1344 1255 1215 834 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3819 5192 6904 Size: 274 80 33 51 1443 1842 1373 1712 Cuts at: 6904 96 Size: 138 Fragments arranged by size: 1842 1712 1443 1373 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 3557 3589 6906 6938 2163 Size: 32 1362 32 3317 32 2171 Fragments arranged by size: 3317 2171 1362 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2194 1316 Size: 878 6068 XbaI T'CTAG_A Cuts at: 3883 4282 3883 Size: 399 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 3449 5075 3449 Size: 1626 5320 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4754 276 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI