(Circular) (Six-base) MAPSORT of: pFN21AB6464.seq Check: 7703 from: 1 to: 5761 pFN21AB6464 5761 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2168 2168 Size: 5761 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2288 3571 278 Size: 53 83 186 936 752 1283 2468 Fragments arranged by size: 2468 1283 936 752 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3060 3888 4261 1563 Size: 1497 828 373 3063 Fragments arranged by size: 3063 1497 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2482 2691 3824 5208 5476 1632 Size: 560 290 209 1133 1384 268 1917 Fragments arranged by size: 1917 1384 1133 560 290 268 209 AfeI AGC'GCT Cuts at: 2059 2059 Size: 5761 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2207 1166 Size: 1041 4720 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2225 1184 Size: 1041 4720 AlwNI CAG_nnn'CTG Cuts at: 2312 3504 5076 2312 Size: 1192 1572 2997 Fragments arranged by size: 2997 1572 1192 ApaI G_GGCC'C Cuts at: 2023 2888 2023 Size: 865 4896 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2414 3070 3296 3448 5557 1493 Size: 921 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 656 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2605 3086 1870 Size: 73 72 590 481 4545 Fragments arranged by size: 4545 590 481 73 72 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 3082 4537 618 Size: 325 217 627 677 618 1455 1842 Fragments arranged by size: 1842 1455 677 627 618 325 217 BanII G_rGCy'C Cuts at: 1391 2023 2595 2888 3080 1391 Size: 632 572 293 192 4072 Fragments arranged by size: 4072 632 572 293 192 BbeI G_GCGC'C Cuts at: 2468 2468 Size: 5761 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BbvCI CC'TCA_GC Cuts at: 2474 2474 Size: 5761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2703 4372 Size: 107 122 71 631 605 376 655 1669 Cuts at: 4372 136 Size: 1525 Fragments arranged by size: 1669 1525 655 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2036 3178 2036 Size: 1142 4619 Bme1580I G_kGCm'C Cuts at: 1790 2023 2888 3822 4978 1790 Size: 233 865 934 1156 2573 Fragments arranged by size: 2573 1156 934 865 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2499 2499 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 4421 1239 Size: 1071 2111 2579 Fragments arranged by size: 2579 2111 1071 Bpu10I CC'TnA_GC Cuts at: 2474 2474 Size: 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2285 2465 3568 3950 Size: 53 83 186 936 752 180 1103 382 Cuts at: 3950 4585 275 Size: 635 1451 Fragments arranged by size: 1451 1103 936 752 635 382 186 180 83 53 BsaWI w'CCGG_w Cuts at: 2613 4194 4592 4866 5013 5424 2613 Size: 1581 398 274 147 411 2950 Fragments arranged by size: 2950 1581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2294 3053 1186 Size: 837 271 759 3894 Fragments arranged by size: 3894 837 759 271 BseYI C'CCAG_C Cuts at: 2212 2701 4964 5460 2212 Size: 489 2263 496 2513 Fragments arranged by size: 2513 2263 496 489 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 3022 1163 Size: 573 381 905 3902 Fragments arranged by size: 3902 905 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2715 3211 3972 5000 1540 Size: 275 186 714 496 761 1028 2301 Fragments arranged by size: 2301 1028 761 714 496 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 3822 3907 4978 2204 Size: 1618 85 1071 2987 Fragments arranged by size: 2987 1618 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2276 2276 Size: 5761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2204 2595 2888 3080 3822 3907 Size: 399 233 181 391 293 192 742 85 Cuts at: 3907 4978 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 293 233 192 181 85 BspEI T'CCGG_A Cuts at: 2613 5424 2613 Size: 2811 2950 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2168 2730 2852 3116 844 Size: 1324 562 122 264 3489 Fragments arranged by size: 3489 1324 562 264 122 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2714 4405 4592 1700 Size: 110 904 1691 187 2869 Fragments arranged by size: 2869 1691 904 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 2921 794 Size: 2127 3634 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2121 3091 3458 3843 3860 5312 5398 5410 Size: 149 970 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 970 385 367 346 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2529 2529 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2355 2547 2763 513 Size: 552 1290 192 216 3511 Fragments arranged by size: 3511 1290 552 216 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2712 3208 Size: 54 1425 50 275 18 315 567 496 Cuts at: 3208 4097 5726 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 567 496 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2712 3208 1537 Size: 275 900 496 4090 Fragments arranged by size: 4090 900 496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 2951 3691 1353 Size: 1278 320 740 3423 Fragments arranged by size: 3423 1278 740 320 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 2278 4348 4722 4868 387 Size: 1064 138 660 29 2070 374 146 1280 Fragments arranged by size: 2070 1280 1064 660 374 146 138 29 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2310 2482 2691 3824 4421 5208 Size: 393 560 118 172 209 1133 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 1133 787 597 560 393 268 209 172 118 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 2884 2885 2019 Size: 1 864 1 4895 Fragments arranged by size: 4895 864 1 1 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2043 2061 2226 2468 4908 1678 Size: 365 18 165 242 2440 2531 Fragments arranged by size: 2531 2440 365 242 165 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2270 2271 2302 2630 Size: 32 135 32 667 31 1 31 328 Cuts at: 2630 2662 2886 2918 4402 4434 4476 4508 1373 Size: 32 224 32 1484 32 42 32 2626 Fragments arranged by size: 2626 1484 667 328 224 135 42 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KasI G'GCGC_C Cuts at: 2464 2464 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3081 4875 5059 1305 Size: 1776 1794 184 2007 Fragments arranged by size: 2007 1794 1776 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 10 Size: 54 1425 343 315 3624 Fragments arranged by size: 3624 1425 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2207 2354 2729 3719 4078 4237 Size: 648 927 114 147 375 990 359 159 Cuts at: 4237 518 Size: 2042 Fragments arranged by size: 2042 990 927 648 375 359 159 147 114 MspA1I CmG'CkG Cuts at: 1606 2473 2701 3177 3854 5002 5247 1606 Size: 867 228 476 677 1148 245 2120 Fragments arranged by size: 2120 1148 867 677 476 245 228 NarI GG'CG_CC Cuts at: 2465 2465 Size: 5761 NcoI C'CATG_G Cuts at: 513 1065 2355 2547 2763 513 Size: 552 1290 192 216 3511 Fragments arranged by size: 3511 1290 552 216 192 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 2512 2573 1348 Size: 1164 61 4536 Fragments arranged by size: 4536 1164 61 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 2518 1108 Size: 1410 4351 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PspOMI G'GGCC_C Cuts at: 2019 2884 2019 Size: 865 4896 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2969 3001 2969 Size: 32 5729 PstI C_TGCA'G Cuts at: 830 2493 2841 3113 830 Size: 1663 348 272 3478 Fragments arranged by size: 3478 1663 348 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2473 2701 2473 Size: 228 5533 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SbfI CC_TGCA'GG Cuts at: 2493 3113 2493 Size: 620 5141 ScaI AGT'ACT Cuts at: 3011 4009 5721 3011 Size: 998 1712 3051 Fragments arranged by size: 3051 1712 998 SfcI C'TryA_G Cuts at: 826 1045 2226 2489 2837 3109 4244 4925 5116 Size: 219 1181 263 348 272 1135 681 191 Cuts at: 5116 826 Size: 1471 Fragments arranged by size: 1471 1181 1135 681 348 272 263 219 191 SfoI GGC'GCC Cuts at: 2466 2466 Size: 5761 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2355 2547 2763 3200 513 Size: 552 1290 192 216 437 3074 Fragments arranged by size: 3074 1290 552 437 216 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 3788 3805 3958 4143 4588 325 Size: 1255 991 1217 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1217 991 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3009 4007 5719 Size: 274 80 33 51 1443 1032 998 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1032 998 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2545 2545 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 2981 3890 2981 Size: 909 4852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2286 3569 276 Size: 53 83 186 936 752 1283 2468 Fragments arranged by size: 2468 1283 936 752 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI