Restriction map for
pFN21AB6464
(Linear) (Six-base) MAPSORT of: KIBB6464.seq Check: 4776 from: 1 to: 1056
KIBB6464.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 163 1056
Size: 163 893
AatII G_ACGT'C
Cuts at: 0 283 1056
Size: 283 773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 477 686 1056
Size: 187 290 209 370
Fragments arranged by size:
370 290 209 187
AfeI AGC'GCT
Cuts at: 0 54 1056
Size: 54 1002
AleI CACnn'nnGTG
Cuts at: 0 202 1056
Size: 202 854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 1056
Size: 220 836
AlwNI CAG_nnn'CTG
Cuts at: 0 307 1056
Size: 307 749
ApaI G_GGCC'C
Cuts at: 0 18 883 1056
Size: 18 865 173
Fragments arranged by size:
865 173 18
ApoI r'AATT_y
Cuts at: 0 409 1056
Size: 409 647
AvaI C'yCGr_G
Cuts at: 0 10 600 1056
Size: 10 590 456
Fragments arranged by size:
590 456 10
BanI G'GyrC_C
Cuts at: 0 459 1056
Size: 459 597
BanII G_rGCy'C
Cuts at: 0 18 590 883 1056
Size: 18 572 293 173
Fragments arranged by size:
572 293 173 18
BbeI G_GCGC'C
Cuts at: 0 463 1056
Size: 463 593
BbvCI CC'TCA_GC
Cuts at: 0 469 1056
Size: 469 587
BglI GCCn_nnn'nGGC
Cuts at: 0 43 698 1056
Size: 43 655 358
Fragments arranged by size:
655 358 43
BlpI GC'TnA_GC
Cuts at: 0 31 1056
Size: 31 1025
Bme1580I G_kGCm'C
Cuts at: 0 18 883 1056
Size: 18 865 173
Fragments arranged by size:
865 173 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 494 1056
Size: 494 562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1056
Size: 305 751
Bpu10I CC'TnA_GC
Cuts at: 0 469 1056
Size: 469 587
BsaHI Gr'CG_yC
Cuts at: 0 280 460 1056
Size: 280 180 596
Fragments arranged by size:
596 280 180
BsaWI w'CCGG_w
Cuts at: 0 608 1056
Size: 608 448
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 289 1048 1056
Size: 18 271 759 8
Fragments arranged by size:
759 271 18 8
BseYI C'CCAG_C
Cuts at: 0 207 696 1056
Size: 207 489 360
Fragments arranged by size:
489 360 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1017 1056
Size: 112 905 39
Fragments arranged by size:
905 112 39
BsiEI CG_ry'CG
Cuts at: 0 710 1056
Size: 710 346
BsiHKAI G_wGCw'C
Cuts at: 0 199 1056
Size: 199 857
BsmBI CGTCTCn'nnnn_
Cuts at: 0 271 1056
Size: 271 785
Bsp1286I G_dGCh'C
Cuts at: 0 18 199 590 883 1056
Size: 18 181 391 293 173
Fragments arranged by size:
391 293 181 173 18
BspEI T'CCGG_A
Cuts at: 0 608 1056
Size: 608 448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 163 725 847 1056
Size: 163 562 122 209
Fragments arranged by size:
562 209 163 122
BsrFI r'CCGG_y
Cuts at: 0 709 1056
Size: 709 347
BstAPI GCAn_nnn'nTGC
Cuts at: 0 916 1056
Size: 916 140
BstYI r'GATC_y
Cuts at: 0 116 1056
Size: 116 940
Bsu36I CC'TnA_GG
Cuts at: 0 524 1056
Size: 524 532
BtgI C'CryG_G
Cuts at: 0 350 542 758 1056
Size: 350 192 216 298
Fragments arranged by size:
350 298 216 192
EaeI y'GGCC_r
Cuts at: 0 140 707 1056
Size: 140 567 349
Fragments arranged by size:
567 349 140
EagI C'GGCC_G
Cuts at: 0 707 1056
Size: 707 349
EarI CTCTTCn'nnn_
Cuts at: 0 626 946 1056
Size: 626 320 110
Fragments arranged by size:
626 320 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 273 1056
Size: 244 29 783
Fragments arranged by size:
783 244 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 305 477 686 1056
Size: 187 118 172 209 370
Fragments arranged by size:
370 209 187 172 118
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 879 880 1056
Size: 14 1 864 1 176
Fragments arranged by size:
864 176 14 1 1
HaeII r_GCGC'y
Cuts at: 0 38 56 221 463 1056
Size: 38 18 165 242 593
Fragments arranged by size:
593 242 165 38 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 265 266 297 625 657 881 913
Size: 234 31 1 31 328 32 224 32
Cuts at: 913 1056
Size: 143
Fragments arranged by size:
328 234 224 143 32 32 31 31
1
KasI G'GCGC_C
Cuts at: 0 459 1056
Size: 459 597
MscI TGG'CCA
Cuts at: 0 142 1056
Size: 142 914
MslI CAynn'nnrTG
Cuts at: 0 88 202 349 724 1056
Size: 88 114 147 375 332
Fragments arranged by size:
375 332 147 114 88
MspA1I CmG'CkG
Cuts at: 0 468 696 1056
Size: 468 228 360
Fragments arranged by size:
468 360 228
NarI GG'CG_CC
Cuts at: 0 460 1056
Size: 460 596
NcoI C'CATG_G
Cuts at: 0 350 542 758 1056
Size: 350 192 216 298
Fragments arranged by size:
350 298 216 192
PasI CC'CwG_GG
Cuts at: 0 507 568 1056
Size: 507 61 488
Fragments arranged by size:
507 488 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 513 1056
Size: 513 543
PspOMI G'GGCC_C
Cuts at: 0 14 879 1056
Size: 14 865 177
Fragments arranged by size:
865 177 14
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 964 996 1056
Size: 964 32 60
Fragments arranged by size:
964 60 32
PstI C_TGCA'G
Cuts at: 0 488 836 1056
Size: 488 348 220
Fragments arranged by size:
488 348 220
PvuII CAG'CTG
Cuts at: 0 468 696 1056
Size: 468 228 360
Fragments arranged by size:
468 360 228
SbfI CC_TGCA'GG
Cuts at: 0 488 1056
Size: 488 568
ScaI AGT'ACT
Cuts at: 0 1006 1056
Size: 1006 50
SfcI C'TryA_G
Cuts at: 0 221 484 832 1056
Size: 221 263 348 224
Fragments arranged by size:
348 263 224 221
SfoI GGC'GCC
Cuts at: 0 461 1056
Size: 461 595
StyI C'CwwG_G
Cuts at: 0 350 542 758 1056
Size: 350 192 216 298
Fragments arranged by size:
350 298 216 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 1056
Size: 566 490
TatI w'GTAC_w
Cuts at: 0 1004 1056
Size: 1004 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 1056
Size: 540 516
XmnI GAAnn'nnTTC
Cuts at: 0 976 1056
Size: 976 80
ZraI GAC'GTC
Cuts at: 0 281 1056
Size: 281 775
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BstAPI BstYI Bsu36I BtgI EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NarI NcoI PasI PflMI PspOMI PsrI PstI PvuII
SbfI ScaI SfcI SfoI StyI TaqII TatI XcmI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI