(Circular) (Six-base) MAPSORT of: pFN21AB5369.seq Check: 5324 from: 1 to: 6277 pFN21AB5369 6277 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2048 3420 2048 Size: 1372 4905 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 1937 Size: 1683 4594 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 4340 5724 5992 1632 Size: 485 2223 1384 268 1917 Fragments arranged by size: 2223 1917 1384 485 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 2679 5176 6153 1203 Size: 1476 2497 977 1327 Fragments arranged by size: 2497 1476 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 2150 2507 1166 Size: 984 357 4936 Fragments arranged by size: 4936 984 357 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 2405 2962 3049 4020 5592 2405 Size: 557 87 971 1572 3090 Fragments arranged by size: 3090 1572 971 557 87 ApaI G_GGCC'C Cuts at: 3401 3401 Size: 6277 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 3586 3812 3964 6073 1493 Size: 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2339 2381 3021 3598 Size: 325 217 627 249 303 42 640 577 Cuts at: 3598 5053 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 640 627 577 325 303 249 217 42 BanII G_rGCy'C Cuts at: 1391 3401 3596 1391 Size: 2010 195 4072 Fragments arranged by size: 4072 2010 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3117 3142 3379 928 Size: 971 1218 25 237 3826 Fragments arranged by size: 3826 1218 971 237 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3158 4169 5379 3158 Size: 1011 1210 4056 Fragments arranged by size: 4056 1210 1011 BclI T'GATC_A Cuts at: 1548 2655 1548 Size: 1107 5170 BfrBI ATG'CAT Cuts at: 2632 2632 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 3044 3509 Size: 107 122 71 631 605 874 498 465 Cuts at: 3509 4888 136 Size: 1379 1525 Fragments arranged by size: 1525 1379 874 631 605 498 465 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2384 2738 3024 3348 3401 4338 5494 1790 Size: 594 354 286 324 53 937 1156 2573 Fragments arranged by size: 2573 1156 937 594 354 324 286 53 BmgBI CAC'GTC Cuts at: 1318 2653 3148 3379 1318 Size: 1335 495 231 4216 Fragments arranged by size: 4216 1335 495 231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 4008 4966 Size: 416 53 83 399 24 1053 1538 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1753 1538 1053 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2464 3313 2464 Size: 849 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2285 4937 1239 Size: 908 138 2652 2579 Fragments arranged by size: 2652 2579 908 138 Bpu10I CC'TnA_GC Cuts at: 3563 3563 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 2614 4402 5267 5565 2207 Size: 407 1788 865 298 2919 Fragments arranged by size: 2919 1788 865 407 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2088 2249 2873 3353 4940 882 Size: 617 589 161 624 480 1587 2219 Fragments arranged by size: 2219 1587 624 617 589 480 161 BsaAI yAC'GTr Cuts at: 493 1204 3451 4068 493 Size: 711 2247 617 2702 Fragments arranged by size: 2702 2247 711 617 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3423 4084 4466 5101 Size: 53 83 186 936 1890 661 382 635 Cuts at: 5101 275 Size: 1451 Fragments arranged by size: 1890 1451 936 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4710 5108 5382 5529 5940 4710 Size: 398 274 147 411 5047 Fragments arranged by size: 5047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2783 2813 1178 Size: 30 165 30 1380 30 4642 Fragments arranged by size: 4642 1380 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 3094 1186 Size: 1081 827 4369 Fragments arranged by size: 4369 1081 827 BseYI C'CCAG_C Cuts at: 2474 2525 5480 5976 2474 Size: 51 2955 496 2775 Fragments arranged by size: 2955 2775 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 3401 1163 Size: 573 705 960 4039 Fragments arranged by size: 4039 960 705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3499 4338 4423 5494 3499 Size: 839 85 1071 4282 Fragments arranged by size: 4282 1071 839 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2384 2738 3024 3348 3401 3499 3596 Size: 399 594 354 286 324 53 98 97 Cuts at: 3596 4338 4423 5494 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 399 354 324 286 98 97 85 53 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2048 3420 3632 844 Size: 1204 1372 212 3489 Fragments arranged by size: 3489 1372 1204 212 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3259 4772 4946 65 Size: 1183 2011 1513 174 1396 Fragments arranged by size: 2011 1513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2336 4921 5108 1700 Size: 110 526 2585 187 2869 Fragments arranged by size: 2869 2585 526 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 2503 2737 3356 3428 4337 5349 2503 Size: 234 619 72 909 1012 3431 Fragments arranged by size: 3431 1012 909 619 234 72 BstAPI GCAn_nnn'nTGC Cuts at: 794 3049 3415 794 Size: 2255 366 3656 Fragments arranged by size: 3656 2255 366 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 2443 1184 Size: 1259 5018 BstXI CCAn_nnnn'nTGG Cuts at: 2246 2246 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2958 3607 3974 4359 4376 5828 5914 5926 Size: 986 649 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 986 649 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 2385 3016 3123 3175 3300 3364 3402 Size: 552 1320 631 107 52 125 64 38 Cuts at: 3402 513 Size: 3388 Fragments arranged by size: 3388 1320 631 552 125 107 64 52 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2599 487 Size: 683 56 1373 4165 Fragments arranged by size: 4165 1373 683 56 BtsI GCAGTG_nn' Cuts at: 3129 3234 3793 4587 4607 3129 Size: 105 559 794 20 4799 Fragments arranged by size: 4799 794 559 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DraIII CAC_nnn'GTG Cuts at: 2851 2851 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2923 3383 5284 809 Size: 507 1607 460 1901 1802 Fragments arranged by size: 1901 1802 1607 507 460 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2668 2949 Size: 54 1425 50 275 18 198 640 281 Cuts at: 2949 3381 3487 3724 4613 6242 8 Size: 432 106 237 889 1629 43 Fragments arranged by size: 1629 1425 889 640 432 281 275 237 198 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2768 2876 4207 1353 Size: 1415 108 1331 3423 Fragments arranged by size: 3423 1415 1331 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2147 2285 4340 4937 5724 5992 Size: 393 485 30 138 2055 597 787 268 Cuts at: 5992 1239 Size: 1524 Fragments arranged by size: 2055 1524 787 597 485 393 268 138 30 EcoNI CCTnn'n_nnAGG Cuts at: 3053 3053 Size: 6277 EcoO109I rG'GnC_Cy Cuts at: 2323 3046 3397 3398 3530 2323 Size: 723 351 1 132 5070 Fragments arranged by size: 5070 723 351 132 1 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 3247 2299 Size: 948 5329 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 2337 2532 5424 1678 Size: 659 195 2892 2531 Fragments arranged by size: 2892 2531 659 195 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2549 2581 3279 3311 4918 Size: 32 135 32 977 32 698 32 1607 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1607 977 698 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 3621 3867 669 Size: 988 281 826 857 246 3079 Fragments arranged by size: 3079 988 857 826 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5391 5575 1305 Size: 4086 184 2007 Fragments arranged by size: 4086 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2951 10 Size: 54 1425 343 198 921 3336 Fragments arranged by size: 3336 1425 921 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2507 2651 4235 4594 4753 518 Size: 648 984 357 144 1584 359 159 2042 Fragments arranged by size: 2042 1584 984 648 359 357 159 144 MspA1I CmG'CkG Cuts at: 1606 3693 4370 5518 5763 1606 Size: 2087 677 1148 245 2120 Fragments arranged by size: 2120 2087 1148 677 245 NaeI GCC'GGC Cuts at: 2338 2338 Size: 6277 NcoI C'CATG_G Cuts at: 513 1065 3300 3402 513 Size: 552 2235 102 3388 Fragments arranged by size: 3388 2235 552 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NgoMIV G'CCGG_C Cuts at: 2336 2336 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NsiI A_TGCA'T Cuts at: 2634 2634 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2683 2890 3635 5180 1132 Size: 97 1454 207 745 1545 2229 Fragments arranged by size: 2229 1545 1454 745 207 97 PasI CC'CwG_GG Cuts at: 1348 2246 2431 1348 Size: 898 185 5194 Fragments arranged by size: 5194 898 185 PciI A'CATG_T Cuts at: 2679 5176 2679 Size: 2497 3780 PflMI CCAn_nnn'nTGG Cuts at: 1108 2088 2851 3454 1108 Size: 980 763 603 3931 Fragments arranged by size: 3931 980 763 603 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PmlI CAC'GTG Cuts at: 3451 3451 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2323 Size: 6277 PshAI GACnn'nnGTC Cuts at: 1613 3125 1613 Size: 1512 4765 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 3397 3397 Size: 6277 PstI C_TGCA'G Cuts at: 830 3529 3629 830 Size: 2699 100 3478 Fragments arranged by size: 3478 2699 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SapI GCTCTTCn'nnn_ Cuts at: 2768 2768 Size: 6277 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 2025 2087 4525 6237 2025 Size: 62 2438 1712 2065 Fragments arranged by size: 2438 2065 1712 62 SfcI C'TryA_G Cuts at: 826 1045 3525 3625 4760 5441 5632 826 Size: 219 2480 100 1135 681 191 1471 Fragments arranged by size: 2480 1471 1135 681 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2593 4381 5282 5544 820 Size: 197 926 279 371 1788 901 262 1553 Fragments arranged by size: 1788 1553 926 901 371 279 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 2591 1517 Size: 1074 5203 StyI C'CwwG_G Cuts at: 513 1065 2466 3300 3393 3402 3716 513 Size: 552 1401 834 93 9 314 3074 Fragments arranged by size: 3074 1401 834 552 314 93 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 2444 4304 4321 4474 4659 5104 Size: 1255 600 264 1860 17 153 185 445 Cuts at: 5104 325 Size: 1498 Fragments arranged by size: 1860 1498 1255 600 445 264 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2023 2085 4523 Size: 274 80 33 51 1443 46 62 2438 Cuts at: 4523 6235 96 Size: 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 62 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2243 2266 3400 2243 Size: 23 1134 5120 Fragments arranged by size: 5120 1134 23 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3230 4406 2777 Size: 453 1176 4648 Fragments arranged by size: 4648 1176 453 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI