################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BcgI BfrI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstAPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 1378 GACGTC GACGT^C AcsI 2491 GAATTT [GA]^AATT[CT] ApoI 2491 GAATTT [GA]^AATT[CT] AvrII 331 CCTAGG C^CTAGG BbeI 1303 GGCGCC GGCGC^C BbrPI 1564 CACGTG CAC^GTG BciVI 1357 GTATCC GTATCC(6/5) BlnI 331 CCTAGG C^CTAGG BsaWI 427 ACCGGA [AT]^CCGG[AT] BsmBI 773 GAGACG CGTCTC(1/5) BsmI 1850 GCATTC GAATGC(1/-1) BspLU11I 2390 ACATGT A^CATGT BsrBI 2606 CCGCTC CCGCTC(-3/-3) BsrDI 2121 CATTGC GCAATG(2/0) BstBI 2698 TTCGAA TT^CGAA HaeII 1303 GGCGCC [GA]GCGC^[CT] HpaI 1867 GTTAAC GTT^AAC KasI 1303 GGCGCC G^GCGCC KspI 672 CCGCGG CCGC^GG NarI 1303 GGCGCC GG^CGCC NcoI 272 CCATGG C^CATGG NspV 2698 TTCGAA TT^CGAA PmaCI 1564 CACGTG CAC^GTG PmlI 1564 CACGTG CAC^GTG PpuMI 2333 AGGTCCC [GA]G^G[AT]CC[CT] SacI 235 GAGCTC GAGCT^C SacII 672 CCGCGG CCGC^GG SalI 1381 GTCGAC G^TCGAC SapI 1269 GCTCTTC GCTCTTC(1/4) SfoI 1303 GGCGCC GGC^GCC SfuI 2698 TTCGAA TT^CGAA SmlI 940 CTTGAG C^T[CT][GA]AG SphI 1256 GCATGC GCATG^C SspI 871 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1381 GTCGAC GT^[AC][GT]AC 1540 GTCTAC AcyI 1303 GGCGCC G[GA]^CG[CT]C 1378 GACGTC AflIII 2390 ACATGT A^C[GA][CT]GT 2582 ACACGT AocI 54 CCTGAGG CC^T[ACGT]AGG 580 CCTGAGG AvaI 766 CCCGAG C^[CT]CG[GA]G 2474 CTCGGG BalI 33 TGGCCA TGG^CCA 1508 TGGCCA BamHI 668 GGATCC G^GATCC 998 GGATCC BbvCI 574 CCTCAGC CCTCAGC(-5/-2) 550 GCTGAGG BclI 1784 TGATCA T^GATCA 2429 TGATCA BfaI 332 CTAG C^TAG 2650 CTAG BglII 1479 AGATCT A^GATCT 2646 AGATCT BsaHI 1303 GGCGCC G[GA]^CG[CT]C 1378 GACGTC BsaI 431 GAGACC GGTCTC(1/5) 1318 GAGACC BsoBI 766 CCCGAG C^[CT]CG[GA]G 2474 CTCGGG BspMI 434 ACCTGC ACCTGC(4/8) 2675 ACCTGC BstEII 813 GGTCACC G^GT[ACGT]ACC 1800 GGTGACC BstPI 813 GGTCACC G^GT[ACGT]ACC 1800 GGTGACC Bsu36I 54 CCTGAGG CC^T[ACGT]AGG 580 CCTGAGG DraIII 70 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG 2260 CACCTTGTG Eco81I 54 CCTGAGG CC^T[ACGT]AGG 580 CCTGAGG EcoO65I 813 GGTCACC G^GT[ACGT]ACC 1800 GGTGACC FbaI 1784 TGATCA T^GATCA 2429 TGATCA HgaI 775 GACGC GACGC(5/10) 1057 GCGTC Hin1I 1303 GGCGCC G[GA]^CG[CT]C 1378 GACGTC MaeI 332 CTAG C^TAG 2650 CTAG MluNI 33 TGGCCA TGG^CCA 1508 TGGCCA MscI 33 TGGCCA TGG^CCA 1508 TGGCCA PstI 255 CTGCAG CTGCA^G 442 CTGCAG ScaI 164 AGTACT AGT^ACT 2279 AGTACT SfcI 255 CTGCAG C^T[GA][CT]AG 442 CTGCAG StuI 707 AGGCCT AGG^CCT 756 AGGCCT XcmI 1266 CCAGCTCTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1514 CCATTGAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII