(Linear) (Six-base) MAPSORT of: KIBB4181.seq Check: 3523 from: 1 to: 2706 KIBB4181.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AatII G_ACGT'C Cuts at: 0 1382 2706 Size: 1382 1324 AccI GT'mk_AC Cuts at: 0 1382 1541 2706 Size: 1382 159 1165 Fragments arranged by size: 1382 1165 159 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 530 737 1576 2706 Size: 530 207 839 1130 Fragments arranged by size: 1130 839 530 207 AflIII A'CryG_T Cuts at: 0 2390 2582 2706 Size: 2390 192 124 Fragments arranged by size: 2390 192 124 AleI CACnn'nnGTG Cuts at: 0 1780 1957 2377 2706 Size: 1780 177 420 329 Fragments arranged by size: 1780 420 329 177 AlwNI CAG_nnn'CTG Cuts at: 0 27 723 1885 2569 2706 Size: 27 696 1162 684 137 Fragments arranged by size: 1162 696 684 137 27 ApoI r'AATT_y Cuts at: 0 2491 2706 Size: 2491 215 AvaI C'yCGr_G Cuts at: 0 766 2474 2706 Size: 766 1708 232 Fragments arranged by size: 1708 766 232 AvrII C'CTAG_G Cuts at: 0 331 2706 Size: 331 2375 BamHI G'GATC_C Cuts at: 0 668 998 2706 Size: 668 330 1708 Fragments arranged by size: 1708 668 330 BanI G'GyrC_C Cuts at: 0 335 687 762 1303 1534 2706 Size: 335 352 75 541 231 1172 Fragments arranged by size: 1172 541 352 335 231 75 BanII G_rGCy'C Cuts at: 0 239 798 1627 2706 Size: 239 559 829 1079 Fragments arranged by size: 1079 829 559 239 BbeI G_GCGC'C Cuts at: 0 1307 2706 Size: 1307 1399 BbsI GAAGACnn'nnnn_ Cuts at: 0 1031 1564 1753 2390 2505 2706 Size: 1031 533 189 637 115 201 Fragments arranged by size: 1031 637 533 201 189 115 BbvCI CC'TCA_GC Cuts at: 0 551 575 2706 Size: 551 24 2131 Fragments arranged by size: 2131 551 24 BciVI GTATCCnnnnn_n' Cuts at: 0 1368 2706 Size: 1368 1338 BclI T'GATC_A Cuts at: 0 1784 2429 2706 Size: 1784 645 277 Fragments arranged by size: 1784 645 277 BglI GCCn_nnn'nGGC Cuts at: 0 32 1762 2291 2706 Size: 32 1730 529 415 Fragments arranged by size: 1730 529 415 32 BglII A'GATC_T Cuts at: 0 1479 2646 2706 Size: 1479 1167 60 Fragments arranged by size: 1479 1167 60 Bme1580I G_kGCm'C Cuts at: 0 291 767 2706 Size: 291 476 1939 Fragments arranged by size: 1939 476 291 BmgBI CAC'GTC Cuts at: 0 2601 2706 Size: 2601 105 BmrI ACTGGGnnnn_n' Cuts at: 0 645 2029 2706 Size: 645 1384 677 Fragments arranged by size: 1384 677 645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1036 1132 2542 2706 Size: 901 135 96 1410 164 Fragments arranged by size: 1410 901 164 135 96 Bpu10I CC'TnA_GC Cuts at: 0 551 575 1796 2706 Size: 551 24 1221 910 Fragments arranged by size: 1221 910 551 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 2706 Size: 961 1745 BsaI GGTCTCn'nnnn_ Cuts at: 0 425 1312 2706 Size: 425 887 1394 Fragments arranged by size: 1394 887 425 BsaAI yAC'GTr Cuts at: 0 1224 1566 2585 2706 Size: 1224 342 1019 121 Fragments arranged by size: 1224 1019 342 121 BsaHI Gr'CG_yC Cuts at: 0 1304 1379 2706 Size: 1304 75 1327 Fragments arranged by size: 1327 1304 75 BsaWI w'CCGG_w Cuts at: 0 427 2706 Size: 427 2279 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 140 170 1192 1222 2706 Size: 140 30 1022 30 1484 Fragments arranged by size: 1484 1022 140 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1213 1516 2422 2706 Size: 1213 303 906 284 Fragments arranged by size: 1213 906 303 284 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 550 1230 2095 2706 Size: 550 680 865 611 Fragments arranged by size: 865 680 611 550 BsiHKAI G_wGCw'C Cuts at: 0 239 464 484 2706 Size: 239 225 20 2222 Fragments arranged by size: 2222 239 225 20 BsmI GAATG_Cn' Cuts at: 0 1850 2706 Size: 1850 856 BsmBI CGTCTCn'nnnn_ Cuts at: 0 767 2706 Size: 767 1939 Bsp1286I G_dGCh'C Cuts at: 0 239 291 464 484 767 798 1627 2706 Size: 239 52 173 20 283 31 829 1079 Fragments arranged by size: 1079 829 283 239 173 52 31 20 BspHI T'CATG_A Cuts at: 0 1973 2157 2426 2706 Size: 1973 184 269 280 Fragments arranged by size: 1973 280 269 184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 443 2684 2706 Size: 443 2241 22 Fragments arranged by size: 2241 443 22 BsrBI CCG'CTC Cuts at: 0 2608 2706 Size: 2608 98 BsrDI GCAATG_nn' Cuts at: 0 2120 2706 Size: 2120 586 BsrFI r'CCGG_y Cuts at: 0 153 645 1457 1846 2706 Size: 153 492 812 389 860 Fragments arranged by size: 860 812 492 389 153 BsrGI T'GTAC_A Cuts at: 0 488 1133 1466 2706 Size: 488 645 333 1240 Fragments arranged by size: 1240 645 488 333 BstBI TT'CG_AA Cuts at: 0 2699 2706 Size: 2699 7 BstEII G'GTnAC_C Cuts at: 0 813 1800 2706 Size: 813 987 906 Fragments arranged by size: 987 906 813 BstXI CCAn_nnnn'nTGG Cuts at: 0 1014 1863 1959 2706 Size: 1014 849 96 747 Fragments arranged by size: 1014 849 747 96 BstYI r'GATC_y Cuts at: 0 668 998 1479 1616 1882 2646 2706 Size: 668 330 481 137 266 764 60 Fragments arranged by size: 764 668 481 330 266 137 60 Bsu36I CC'TnA_GG Cuts at: 0 55 581 2706 Size: 55 526 2125 Fragments arranged by size: 2125 526 55 BtgI C'CryG_G Cuts at: 0 272 672 2235 2486 2706 Size: 272 400 1563 251 220 Fragments arranged by size: 1563 400 272 251 220 BtsI GCAGTG_nn' Cuts at: 0 17 322 939 1241 1397 2706 Size: 17 305 617 302 156 1309 Fragments arranged by size: 1309 617 305 302 156 17 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2475 2510 2706 Size: 2475 35 196 Fragments arranged by size: 2475 196 35 DraIII CAC_nnn'GTG Cuts at: 0 75 2265 2706 Size: 75 2190 441 Fragments arranged by size: 2190 441 75 EaeI y'GGCC_r Cuts at: 0 33 350 802 1394 1508 1899 2341 2603 Size: 33 317 452 592 114 391 442 262 Cuts at: 2603 2706 Size: 103 Fragments arranged by size: 592 452 442 391 317 262 114 103 33 EarI CTCTTCn'nnn_ Cuts at: 0 508 1276 2180 2706 Size: 508 768 904 526 Fragments arranged by size: 904 768 526 508 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 424 2706 Size: 424 2282 EcoICRI GAG'CTC Cuts at: 0 237 2706 Size: 237 2469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 530 737 901 1036 1132 1576 2542 2706 Size: 530 207 164 135 96 444 966 164 Fragments arranged by size: 966 530 444 207 164 164 135 96 EcoNI CCTnn'n_nnAGG Cuts at: 0 58 584 1674 2065 2342 2706 Size: 58 526 1090 391 277 364 Fragments arranged by size: 1090 526 391 364 277 58 EcoO109I rG'GnC_Cy Cuts at: 0 344 1521 2334 2535 2706 Size: 344 1177 813 201 171 Fragments arranged by size: 1177 813 344 201 171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1428 2706 Size: 1428 1278 HaeII r_GCGC'y Cuts at: 0 1307 2706 Size: 1307 1399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 818 850 1311 1343 2421 2453 2706 Size: 818 32 461 32 1078 32 253 Fragments arranged by size: 1078 818 461 253 32 32 32 HincII GTy'rAC Cuts at: 0 1383 1869 2580 2706 Size: 1383 486 711 126 Fragments arranged by size: 1383 711 486 126 HpaI GTT'AAC Cuts at: 0 1869 2706 Size: 1869 837 KasI G'GCGC_C Cuts at: 0 1303 2706 Size: 1303 1403 MscI TGG'CCA Cuts at: 0 35 1510 2706 Size: 35 1475 1196 Fragments arranged by size: 1475 1196 35 MslI CAynn'nnrTG Cuts at: 0 271 1780 1922 1957 2377 2706 Size: 271 1509 142 35 420 329 Fragments arranged by size: 1509 420 329 271 142 35 MspA1I CmG'CkG Cuts at: 0 674 879 996 1167 1753 2217 2706 Size: 674 205 117 171 586 464 489 Fragments arranged by size: 674 586 489 464 205 171 117 NaeI GCC'GGC Cuts at: 0 647 1459 1848 2706 Size: 647 812 389 858 Fragments arranged by size: 858 812 647 389 NarI GG'CG_CC Cuts at: 0 1304 2706 Size: 1304 1402 NcoI C'CATG_G Cuts at: 0 272 2706 Size: 272 2434 NgoMIV G'CCGG_C Cuts at: 0 645 1457 1846 2706 Size: 645 812 389 860 Fragments arranged by size: 860 812 645 389 NspI r_CATG'y Cuts at: 0 1260 1575 2394 2706 Size: 1260 315 819 312 Fragments arranged by size: 1260 819 315 312 PasI CC'CwG_GG Cuts at: 0 123 2706 Size: 123 2583 PciI A'CATG_T Cuts at: 0 2390 2706 Size: 2390 316 PflMI CCAn_nnn'nTGG Cuts at: 0 75 225 359 404 1885 2706 Size: 75 150 134 45 1481 821 Fragments arranged by size: 1481 821 150 134 75 45 PfoI T'CCnGG_A Cuts at: 0 464 1013 1741 2706 Size: 464 549 728 965 Fragments arranged by size: 965 728 549 464 PmlI CAC'GTG Cuts at: 0 1566 2706 Size: 1566 1140 PpuMI rG'GwC_Cy Cuts at: 0 2334 2706 Size: 2334 372 PstI C_TGCA'G Cuts at: 0 259 446 2706 Size: 259 187 2260 Fragments arranged by size: 2260 259 187 PvuII CAG'CTG Cuts at: 0 879 1167 1753 2706 Size: 879 288 586 953 Fragments arranged by size: 953 879 586 288 SacI G_AGCT'C Cuts at: 0 239 2706 Size: 239 2467 SacII CC_GC'GG Cuts at: 0 675 2706 Size: 675 2031 SalI G'TCGA_C Cuts at: 0 1381 2706 Size: 1381 1325 SapI GCTCTTCn'nnn_ Cuts at: 0 1276 2706 Size: 1276 1430 ScaI AGT'ACT Cuts at: 0 166 2281 2706 Size: 166 2115 425 Fragments arranged by size: 2115 425 166 SfcI C'TryA_G Cuts at: 0 255 442 2706 Size: 255 187 2264 Fragments arranged by size: 2264 255 187 SfoI GGC'GCC Cuts at: 0 1305 2706 Size: 1305 1401 SmlI C'TyrA_G Cuts at: 0 940 2706 Size: 940 1766 SphI G_CATG'C Cuts at: 0 1260 2706 Size: 1260 1446 SspI AAT'ATT Cuts at: 0 873 2706 Size: 873 1833 StuI AGG'CCT Cuts at: 0 709 758 2706 Size: 709 49 1948 Fragments arranged by size: 1948 709 49 StyI C'CwwG_G Cuts at: 0 272 331 1403 2402 2706 Size: 272 59 1072 999 304 Fragments arranged by size: 1072 999 304 272 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 70 2611 2706 Size: 70 2541 95 Fragments arranged by size: 2541 95 70 TatI w'GTAC_w Cuts at: 0 164 488 1133 1466 2279 2706 Size: 164 324 645 333 813 427 Fragments arranged by size: 813 645 427 333 324 164 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1192 1224 1425 1457 2706 Size: 1192 32 201 32 1249 Fragments arranged by size: 1249 1192 201 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1273 1521 2706 Size: 1273 248 1185 Fragments arranged by size: 1273 1185 248 ZraI GAC'GTC Cuts at: 0 1380 2706 Size: 1380 1326 Enzymes that do cut: AatII AccI AcuI AflIII AleI AlwNI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HpaI KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI SacII SalI SapI ScaI SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI BcgI BfrBI BlpI BmtI BplI BsaBI BseYI BsiEI BsiWI BspEI BssHII BssSI BstAPI BstZ17I BtgZI ClaI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HindIII KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI