Restriction map for
pFN21AE6206
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 501 AAATTC [GA]^AATT[CT]
AfaI 581 GTAC GT^AC
AflIII 276 ACATGT A^C[GA][CT]GT
AluI 524 AGCT AG^CT
AlwI 320 GGATC GGATC(4/5)
ApoI 501 AAATTC [GA]^AATT[CT]
AvaI 367 CCCGAG C^[CT]CG[GA]G
BbrPI 166 CACGTG CAC^GTG
BcnI 395 CCGGG CC^[GC]GG
BfaI 208 CTAG C^TAG
BglI 366 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 361 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 361 GCTGAGC GC^T[ACGT]AGC
BsaI 76 GAGACC GGTCTC(1/5)
BsaWI 20 ACCGGA [AT]^CCGG[AT]
BsgI 385 CTGCAC GTGCAG(16/14)
BsoBI 367 CCCGAG C^[CT]CG[GA]G
BspHI 413 TCATGA T^CATGA
BspLU11I 276 ACATGT A^CATGT
BssHII 331 GCGCGC G^CGCGC
BstYI 320 GGATCT [GA]^GATC[CT]
Cac8I 331 GCGCGC GC[ACGT]^[ACGT]GC
CelII 361 GCTGAGC GC^T[ACGT]AGC
DpnII 321 GATC ^GATC
DraII 203 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 546 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 203 GGGACCT [GA]G^G[ACGT]CC[CT]
MaeI 208 CTAG C^TAG
MboI 321 GATC ^GATC
MflI 320 GGATCT [GA]^GATC[CT]
NciI 395 CCGGG CC^[GC]GG
NdeII 321 GATC ^GATC
NspI 276 ACATGT [GA]CATG^[CT]
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
PpuMI 203 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 413 TCATGA T^CATGA
RsaI 581 GTAC GT^AC
SapI 177 GCTCTTC GCTCTTC(1/4)
Sau3AI 321 GATC ^GATC
SexAI 235 ACCTGGT A^CC[AT]GGT
SfaNI 533 GCATC GCATC(5/9)
SnaBI 91 TACGTA TAC^GTA
XhoII 320 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 204 GGACC G^G[AT]CC
351 GGACC
BanII 252 GAGCCC G[GA]GC[CT]^C
364 GAGCCC
BpmI 516 CTCCAG CTGGAG(16/14)
249 CTGGAG
BsaAI 91 TACGTA [CT]AC^GT[GA]
166 CACGTG
BsmBI 511 CGTCTC CGTCTC(1/5)
569 GAGACG
BsmFI 203 GGGAC GGGAC(10/14)
588 GGGAC
BsrI 129 ACTGG ACTGG(1/-1)
200 ACTGG
BstF5I 119 CATCC GGATG(2/0)
534 CATCC
Cfr13I 204 GGACC G^G[ACGT]CC
351 GGACC
EaeI 216 TGGCCG [CT]^GGCC[GA]
281 TGGCCG
EarI 178 CTCTTC CTCTTC(1/4)
99 GAAGAG
FokI 119 CATCC GGATG(9/13)
534 CATCC
HaeIII 217 GGCC GG^CC
282 GGCC
HapII 21 CCGG C^CGG
395 CCGG
HgaI 571 GACGC GACGC(5/10)
230 GCGTC
HpaII 21 CCGG C^CGG
395 CCGG
Ksp632I 178 CTCTTC CTCTTC(1/4)
99 GAAGAG
MaeII 92 ACGT A^CGT
167 ACGT
MaeIII 107 GTGAC ^GT[ACGT]AC
232 GTCAC
MspA1I 54 CCGCTG C[AC]G^C[GT]G
219 CCGCTG
MspI 21 CCGG C^CGG
395 CCGG
Sau96I 204 GGACC G^G[ACGT]CC
351 GGACC
Tsp45I 107 GTGAC ^GT[GC]AC
232 GTCAC
TspRI 111 CAGTG CA[GC]TG[ACGT][ACGT]^
128 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AccII AciI BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI BstUI CfoI DdeI EcoRII
Fnu4HI HhaI HinP1I HphI ItaI
MboII MnlI MvaI MvnI MwoI
NlaIII NlaIV ScrFI TseI Tsp509I