Restriction map for
pFN21AE0352
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbsI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsgI BsiEI
BsiWI BsmBI BsmI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 15 CGCG CG^CG
AccIII 1233 TCCGGA T^CCGGA
AspI 1270 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 108 GGCGCC G^G[CT][GA]CC
BanII 506 GGGCTC G[GA]GC[CT]^C
BbeI 108 GGCGCC GGCGC^C
BbvCI 571 GCTGAGG CCTCAGC(-5/-2)
BciVI 720 GTATCC GTATCC(6/5)
BglI 499 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 181 AGATCT A^GATCT
BpmI 578 CTGGAG CTGGAG(16/14)
BsaWI 1233 TCCGGA [AT]^CCGG[AT]
BseAI 1233 TCCGGA T^CCGGA
BseRI 40 GAGGAG GAGGAG(10/8)
BsmFI 1545 GGGAC GGGAC(10/14)
Bsp1407I 451 TGTACA T^GTACA
BspDI 1210 ATCGAT AT^CGAT
BspEI 1233 TCCGGA T^CCGGA
BspLU11I 377 ACATGT A^CATGT
BsrGI 451 TGTACA T^GTACA
BstAPI 968 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 15 CGCG CG^CG
CfoI 109 GCGC GCG^C
ClaI 1210 ATCGAT AT^CGAT
DraIII 491 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1308 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 108 GGCGCC [GA]GCGC^[CT]
HhaI 109 GCGC GCG^C
HinP1I 109 GCGC G^CGC
KasI 108 GGCGCC G^GCGCC
MfeI 1004 CAATTG C^AATTG
MroI 1233 TCCGGA T^CCGGA
MslI 912 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1004 CAATTG C^AATTG
MvnI 15 CGCG CG^CG
NarI 108 GGCGCC GG^CGCC
NlaIV 108 GGCGCC GG[ACGT]^[ACGT]CC
PflFI 1270 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 230 CAGCTG CAG^CTG
ScaI 80 AGTACT AGT^ACT
SfoI 108 GGCGCC GGC^GCC
SmaI 28 CCCGGG CCC^GGG
SspBI 451 TGTACA T^GTACA
StuI 1046 AGGCCT AGG^CCT
Tth111I 1270 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 668 CCAACATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1009 GTCTAC GT^[AC][GT]AC
1322 GTCTAC
AcsI 157 AAATTC [GA]^AATT[CT]
340 GAATTT
AflIII 377 ACATGT A^C[GA][CT]GT
728 ACGTGT
ApoI 157 AAATTC [GA]^AATT[CT]
340 GAATTT
AvaII 908 GGACC G^G[AT]CC
1269 GGACC
BalI 92 TGGCCA TGG^CCA
1282 TGGCCA
BcgI 17 CGATGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1149 GCACTTGGATCG
BstYI 181 AGATCT [GA]^GATC[CT]
740 AGATCC
EarI 757 GAAGAG CTCTTC(1/4)
903 GAAGAG
Ksp632I 757 GAAGAG CTCTTC(1/4)
903 GAAGAG
MflI 181 AGATCT [GA]^GATC[CT]
740 AGATCC
MluNI 92 TGGCCA TGG^CCA
1282 TGGCCA
MscI 92 TGGCCA TGG^CCA
1282 TGGCCA
MseI 697 TTAA T^TAA
734 TTAA
NspI 140 ACATGC [GA]CATG^[CT]
377 ACATGT
PleI 1547 GACTC GAGTC(4/5)
607 GAGTC
PstI 560 CTGCAG CTGCA^G
1042 CTGCAG
TfiI 838 GATTC G^A[AT]TC
883 GAATC
Tru9I 697 TTAA T^TAA
734 TTAA
XhoII 181 AGATCT [GA]^GATC[CT]
740 AGATCC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
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AciI AcyI AfaI AluI AlwI
AspHI AvaI BbvI BcnI BmyI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I