Restriction map for
pFN21AE0352
(Circular) (Six-base) MAPSORT of: pFN21AE0352.seq Check: 944 from: 1 to: 6295
pFN21AE0352 6295 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3328 3638 1937
Size: 1078 313 310 4594
Fragments arranged by size:
4594 1078 313 310
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2927 4358 5742 6010 1632
Size: 440 855 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 855 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2382 2733 5194 6171 1203
Size: 1179 351 2461 977 1327
Fragments arranged by size:
2461 1327 1179 977 351
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 2921 1166
Size: 1755 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2162 2345 3604 3830 3982 6091 1493
Size: 669 183 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 669 226 183 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2277 3422 3620 1870
Size: 73 90 244 1145 198 4545
Fragments arranged by size:
4545 1145 244 198 90 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3163 3196 3163
Size: 33 6262
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 3616 5071 618
Size: 325 217 627 326 1503 1455 1842
Fragments arranged by size:
1842 1503 1455 627 326 325 217
BanII G_rGCy'C
Cuts at: 1391 2515 3614 1391
Size: 1124 1099 4072
Fragments arranged by size:
4072 1124 1099
BbeI G_GCGC'C
Cuts at: 2117 2117
Size: 6295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5324
BbvCI CC'TCA_GC
Cuts at: 2577 2577
Size: 6295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2011 2045 3143 3177 4486 4520 1903
Size: 34 74 34 1098 34 1309 34 3678
Fragments arranged by size:
3678 1309 1098 74 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 4187 5397 2736
Size: 1451 1210 3634
Fragments arranged by size:
3634 1451 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2510 4906 136
Size: 107 122 71 631 605 838 2396 1525
Fragments arranged by size:
2396 1525 838 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2186 6290 2186
Size: 4104 2191
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2506 4356 5512 1790
Size: 716 1850 1156 2573
Fragments arranged by size:
2573 1850 1156 716
BmgBI CAC'GTC
Cuts at: 1318 3249 1318
Size: 1931 4364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2719 4026 4984
Size: 416 53 83 399 24 1302 1307 958
Cuts at: 4984 442
Size: 1753
Fragments arranged by size:
1753 1307 1302 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 3326 2104
Size: 1222 5073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2604 4955 1239
Size: 1365 2351 2579
Fragments arranged by size:
2579 2351 1365
Bpu10I CC'TnA_GC
Cuts at: 2415 2577 2415
Size: 162 6133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2114 2353 4102
Size: 53 83 186 936 543 38 239 1749
Cuts at: 4102 4484 5119 275
Size: 382 635 1451
Fragments arranged by size:
1749 1451 936 635 543 382 239 186
83 53 38
BsaWI w'CCGG_w
Cuts at: 3238 4728 5126 5400 5547 5958 3238
Size: 1490 398 274 147 411 3575
Fragments arranged by size:
3575 1490 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2577 2607 2997 3027 3233
Size: 30 165 30 1174 30 390 30 206
Cuts at: 3233 3263 1178
Size: 30 4210
Fragments arranged by size:
4210 1174 390 206 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 5421
BseYI C'CCAG_C
Cuts at: 2807 5498 5994 2807
Size: 2691 496 3108
Fragments arranged by size:
3108 2691 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5722
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2416 3046 3156 4356 4441 5512 2416
Size: 630 110 1200 85 1071 3199
Fragments arranged by size:
3199 1200 1071 630 110 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2416 2506 2515 3046 3156 3614 4356
Size: 399 626 90 9 531 110 458 742
Cuts at: 4356 4441 5512 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 531 458 399 110
90 85 9
BspEI T'CCGG_A
Cuts at: 3238 5958 3238
Size: 2720 3575
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3650 844
Size: 2806 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2456 96
Size: 2360 3935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2979 794
Size: 2185 4110
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 2186 2745 3625 3992 4377 4394 5846 5932
Size: 214 559 880 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 559 385 367 346 214
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 487
Size: 683 56 810 4746
Fragments arranged by size:
4746 810 683 56
BtsI GCAGTG_nn'
Cuts at: 2598 3811 4605 4625 2598
Size: 1213 794 20 4268
Fragments arranged by size:
4268 1213 794 20
ClaI AT'CG_AT
Cuts at: 1554 3216 1554
Size: 1662 4633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 2501 2501
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2179 2826
Size: 54 1425 50 275 18 267 82 647
Cuts at: 2826 3287 3742 4631 6260 8
Size: 461 455 889 1629 43
Fragments arranged by size:
1629 1425 889 647 461 455 275 267
82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 2903 4225 1353
Size: 1404 146 1322 3423
Fragments arranged by size:
3423 1404 1322 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2604 2927 4358 4955 5742 6010
Size: 393 440 532 323 1431 597 787 268
Cuts at: 6010 1239
Size: 1524
Fragments arranged by size:
1524 1431 787 597 532 440 393 323
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6295
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 2117 5442 1678
Size: 439 3325 2531
Fragments arranged by size:
3325 2531 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2997 3029 4936 4968 5010
Size: 32 135 32 1425 32 1907 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2626 1907 1425 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KasI G'GCGC_C
Cuts at: 2113 2113
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3009 3894 3009
Size: 885 5410
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3228 5409 5593 1305
Size: 1923 2181 184 2007
Fragments arranged by size:
2181 2007 1923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 3289 10
Size: 54 1425 343 267 1190 3016
Fragments arranged by size:
3016 1425 1190 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2921 4253 4612 4771 518
Size: 648 843 912 1332 359 159 2042
Fragments arranged by size:
2042 1332 912 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2237 2480 2523 2646 3711 4388 5536
Size: 423 208 243 43 123 1065 677 1148
Cuts at: 5536 5781 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1065 677 423 245 243 208
123 43
NarI GG'CG_CC
Cuts at: 2114 2114
Size: 6295
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5743
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2149 2386 3653 5198 1132
Size: 97 920 237 1267 1545 2229
Fragments arranged by size:
2229 1545 1267 920 237 97
PasI CC'CwG_GG
Cuts at: 1348 2506 1348
Size: 1158 5137
PciI A'CATG_T
Cuts at: 2382 5194 2382
Size: 2812 3483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PfoI T'CCnGG_A
Cuts at: 2682 2682
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 2718 3865 2718
Size: 1147 5148
PstI C_TGCA'G
Cuts at: 830 2569 3051 3647 830
Size: 1739 482 596 3478
Fragments arranged by size:
3478 1739 596 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2237 2237
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 2087 4543 6255 2087
Size: 2456 1712 2127
Fragments arranged by size:
2456 2127 1712
SfcI C'TryA_G
Cuts at: 826 1045 2227 2565 3047 3643 4778 5459 5650
Size: 219 1182 338 482 596 1135 681 191
Cuts at: 5650 826
Size: 1471
Fragments arranged by size:
1471 1182 1135 681 596 482 338 219
191
SfoI GGC'GCC
Cuts at: 2115 2115
Size: 6295
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2035 3622 1872
Size: 163 1587 4545
Fragments arranged by size:
4545 1587 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 3053 1517
Size: 1536 4759
StyI C'CwwG_G
Cuts at: 513 1065 2061 2109 2301 2430 3734 513
Size: 552 996 48 192 129 1304 3074
Fragments arranged by size:
3074 1304 996 552 192 129 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2652 4322 4339 4492 4677 5122 325
Size: 1255 1072 1670 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 1072 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2456 2874
Size: 274 80 33 51 1443 108 371 418
Cuts at: 2874 3447 4541 6253 96
Size: 573 1094 1712 138
Fragments arranged by size:
1712 1443 1094 573 418 371 274 138
108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2577 2609 2803 2835 3233 3265 6255 6287 2577
Size: 32 194 32 398 32 2990 32 2585
Fragments arranged by size:
2990 2585 398 194 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3278 1316
Size: 1962 4333
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 2033 3620 1870
Size: 163 1587 4545
Fragments arranged by size:
4545 1587 163
XmnI GAAnn'nnTTC
Cuts at: 4424 4424
Size: 6295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI