Restriction map for
pFN21AE0352
(Linear) (Six-base) MAPSORT of: KIEE0352.seq Check: 1275 from: 1 to: 1590
KIEE0352.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1010 1323 1590
Size: 1010 313 267
Fragments arranged by size:
1010 313 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 922 1590
Size: 67 855 668
Fragments arranged by size:
855 668 67
AflIII A'CryG_T
Cuts at: 0 377 728 1590
Size: 377 351 862
Fragments arranged by size:
862 377 351
AleI CACnn'nnGTG
Cuts at: 0 916 1590
Size: 916 674
ApoI r'AATT_y
Cuts at: 0 157 340 1590
Size: 157 183 1250
Fragments arranged by size:
1250 183 157
AvaI C'yCGr_G
Cuts at: 0 28 272 1417 1590
Size: 28 244 1145 173
Fragments arranged by size:
1145 244 173 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1158 1191 1590
Size: 1158 33 399
Fragments arranged by size:
1158 399 33
BanI G'GyrC_C
Cuts at: 0 108 1590
Size: 108 1482
BanII G_rGCy'C
Cuts at: 0 510 1590
Size: 510 1080
BbeI G_GCGC'C
Cuts at: 0 112 1590
Size: 112 1478
BbvCI CC'TCA_GC
Cuts at: 0 572 1590
Size: 572 1018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6 40 1138 1172 1590
Size: 6 34 1098 34 418
Fragments arranged by size:
1098 418 34 34 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 731 1590
Size: 731 859
BglI GCCn_nnn'nGGC
Cuts at: 0 505 1590
Size: 505 1085
BglII A'GATC_T
Cuts at: 0 181 1590
Size: 181 1409
Bme1580I G_kGCm'C
Cuts at: 0 501 1590
Size: 501 1089
BmgBI CAC'GTC
Cuts at: 0 1244 1590
Size: 1244 346
BmrI ACTGGGnnnn_n'
Cuts at: 0 714 1590
Size: 714 876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1321 1590
Size: 99 1222 269
Fragments arranged by size:
1222 269 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1590
Size: 599 991
Bpu10I CC'TnA_GC
Cuts at: 0 410 572 1590
Size: 410 162 1018
Fragments arranged by size:
1018 410 162
BsaHI Gr'CG_yC
Cuts at: 0 71 109 348 1590
Size: 71 38 239 1242
Fragments arranged by size:
1242 239 71 38
BsaWI w'CCGG_w
Cuts at: 0 1233 1590
Size: 1233 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 572 602 992 1022 1228 1258 1590
Size: 572 30 390 30 206 30 332
Fragments arranged by size:
572 390 332 206 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1590
Size: 55 1535
BseYI C'CCAG_C
Cuts at: 0 802 1590
Size: 802 788
BsiHKAI G_wGCw'C
Cuts at: 0 411 1041 1151 1590
Size: 411 630 110 439
Fragments arranged by size:
630 439 411 110
Bsp1286I G_dGCh'C
Cuts at: 0 411 501 510 1041 1151 1590
Size: 411 90 9 531 110 439
Fragments arranged by size:
531 439 411 110 90 9
BspEI T'CCGG_A
Cuts at: 0 1233 1590
Size: 1233 357
BsrGI T'GTAC_A
Cuts at: 0 451 1590
Size: 451 1139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 974 1590
Size: 974 616
BstYI r'GATC_y
Cuts at: 0 181 740 1590
Size: 181 559 850
Fragments arranged by size:
850 559 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 1590
Size: 31 1559
BtsI GCAGTG_nn'
Cuts at: 0 593 1590
Size: 593 997
ClaI AT'CG_AT
Cuts at: 0 1211 1590
Size: 1211 379
DraIII CAC_nnn'GTG
Cuts at: 0 496 1590
Size: 496 1094
EaeI y'GGCC_r
Cuts at: 0 92 174 821 1282 1590
Size: 92 82 647 461 308
Fragments arranged by size:
647 461 308 92 82
EarI CTCTTCn'nnn_
Cuts at: 0 752 898 1590
Size: 752 146 692
Fragments arranged by size:
752 692 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 599 922 1590
Size: 67 532 323 668
Fragments arranged by size:
668 532 323 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 1590
Size: 1312 278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 1590
Size: 102 1488
HaeII r_GCGC'y
Cuts at: 0 112 1590
Size: 112 1478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1590
Size: 992 32 566
Fragments arranged by size:
992 566 32
KasI G'GCGC_C
Cuts at: 0 108 1590
Size: 108 1482
MfeI C'AATT_G
Cuts at: 0 1004 1590
Size: 1004 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1590
Size: 1223 367
MscI TGG'CCA
Cuts at: 0 94 1284 1590
Size: 94 1190 306
Fragments arranged by size:
1190 306 94
MslI CAynn'nnrTG
Cuts at: 0 916 1590
Size: 916 674
MspA1I CmG'CkG
Cuts at: 0 24 232 475 518 641 1590
Size: 24 208 243 43 123 949
Fragments arranged by size:
949 243 208 123 43 24
NarI GG'CG_CC
Cuts at: 0 109 1590
Size: 109 1481
NspI r_CATG'y
Cuts at: 0 144 381 1590
Size: 144 237 1209
Fragments arranged by size:
1209 237 144
PasI CC'CwG_GG
Cuts at: 0 501 1590
Size: 501 1089
PciI A'CATG_T
Cuts at: 0 377 1590
Size: 377 1213
PfoI T'CCnGG_A
Cuts at: 0 677 1590
Size: 677 913
PsiI TTA'TAA
Cuts at: 0 713 1590
Size: 713 877
PstI C_TGCA'G
Cuts at: 0 564 1046 1590
Size: 564 482 544
Fragments arranged by size:
564 544 482
PvuII CAG'CTG
Cuts at: 0 232 1590
Size: 232 1358
ScaI AGT'ACT
Cuts at: 0 82 1590
Size: 82 1508
SfcI C'TryA_G
Cuts at: 0 222 560 1042 1590
Size: 222 338 482 548
Fragments arranged by size:
548 482 338 222
SfoI GGC'GCC
Cuts at: 0 110 1590
Size: 110 1480
SmaI CCC'GGG
Cuts at: 0 30 1590
Size: 30 1560
StuI AGG'CCT
Cuts at: 0 1048 1590
Size: 1048 542
StyI C'CwwG_G
Cuts at: 0 56 104 296 425 1590
Size: 56 48 192 129 1165
Fragments arranged by size:
1165 192 129 56 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 647 1590
Size: 647 943
TatI w'GTAC_w
Cuts at: 0 80 451 869 1442 1590
Size: 80 371 418 573 148
Fragments arranged by size:
573 418 371 148 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 572 604 798 830 1228 1260 1590
Size: 572 32 194 32 398 32 330
Fragments arranged by size:
572 398 330 194 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1273 1590
Size: 1273 317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 1590
Size: 675 915
XmaI C'CCGG_G
Cuts at: 0 28 1590
Size: 28 1562
Enzymes that do cut:
AccI AcuI AflIII AleI ApoI AvaI BaeI BanI
BanII BbeI BbvCI BcgI BciVI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspEI BsrGI BstAPI BstYI
BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI EcoNI
FalI HaeII Hin4I KasI MfeI MmeI MscI MslI
MspA1I NarI NspI PasI PciI PfoI PsiI PstI
PvuII ScaI SfcI SfoI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbsI BclI BfrBI BlpI BmtI BpuEI
BsaI BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraI DrdI
EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI