(Circular) (Six-base) MAPSORT of: pFN21AE0436.seq Check: 4489 from: 1 to: 5797 pFN21AE0436 5797 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2175 3140 1937 Size: 238 965 4594 Fragments arranged by size: 4594 965 238 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 2854 3924 4297 1563 Size: 1291 1070 373 3063 Fragments arranged by size: 3063 1291 1070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 5244 5512 1632 Size: 2228 1384 268 1917 Fragments arranged by size: 2228 1917 1384 268 AfeI AGC'GCT Cuts at: 2151 2151 Size: 5797 AflII C'TTAA_G Cuts at: 820 1017 2986 820 Size: 197 1969 3631 Fragments arranged by size: 3631 1969 197 AflIII A'CryG_T Cuts at: 1203 2028 2088 2344 2608 4696 5673 1203 Size: 825 60 256 264 2088 977 1327 Fragments arranged by size: 2088 1327 977 825 264 256 60 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2087 1166 Size: 921 4876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 2441 2750 2775 2885 3106 3332 3484 5593 Size: 948 309 25 110 221 226 152 2109 Cuts at: 5593 1493 Size: 1697 Fragments arranged by size: 2109 1697 948 309 226 221 152 110 25 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3118 4573 618 Size: 325 217 627 290 1041 1455 1842 Fragments arranged by size: 1842 1455 1041 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 3116 1391 Size: 1725 4072 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 5797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2969 928 Size: 971 1070 3756 Fragments arranged by size: 3756 1070 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3988 4022 1903 Size: 34 50 34 1967 34 3678 Fragments arranged by size: 3678 1967 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2406 3214 2406 Size: 808 4989 Bme1580I G_kGCm'C Cuts at: 1790 2075 3858 5014 1790 Size: 285 1783 1156 2573 Fragments arranged by size: 2573 1783 1156 285 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4457 1239 Size: 3218 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2031 2089 2356 2482 3588 493 Size: 711 827 58 267 126 1106 2702 Fragments arranged by size: 2702 1106 827 711 267 126 58 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 3604 3986 4621 Size: 53 83 186 936 545 1526 382 635 Cuts at: 4621 275 Size: 1451 Fragments arranged by size: 1526 1451 936 635 545 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 1178 Size: 30 165 30 615 30 4927 Fragments arranged by size: 4927 615 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5797 BseYI C'CCAG_C Cuts at: 2054 5000 5496 2054 Size: 2946 496 2355 Fragments arranged by size: 2946 2355 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2161 3247 4008 5036 1540 Size: 275 186 160 1086 761 1028 2301 Fragments arranged by size: 2301 1086 1028 761 275 186 160 BsiHKAI G_wGCw'C Cuts at: 2025 2067 2193 3858 3943 5014 2025 Size: 42 126 1665 85 1071 2808 Fragments arranged by size: 2808 1665 1071 126 85 42 BsmI GAATG_Cn' Cuts at: 2338 3308 3401 2338 Size: 970 93 4734 Fragments arranged by size: 4734 970 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2067 2075 2193 3116 3858 3943 Size: 399 235 42 8 118 923 742 85 Cuts at: 3943 5014 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 923 742 399 235 118 85 42 8 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 3152 844 Size: 1211 1097 3489 Fragments arranged by size: 3489 1211 1097 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 4441 4628 1700 Size: 110 350 2281 187 2869 Fragments arranged by size: 2869 2281 350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 2579 794 Size: 1785 4012 BstBI TT'CG_AA Cuts at: 2859 4601 2859 Size: 1742 4055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2902 3127 3494 3879 3896 5348 5434 5446 Size: 930 225 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 930 385 367 346 225 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3313 4107 4127 2440 Size: 873 794 20 4110 Fragments arranged by size: 4110 873 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 3244 4133 Size: 54 1425 50 275 18 489 925 889 Cuts at: 4133 5762 8 Size: 1629 43 Fragments arranged by size: 1629 1425 925 889 489 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2722 3727 1353 Size: 845 524 1005 3423 Fragments arranged by size: 3423 1005 845 524 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 4384 4758 4904 387 Size: 1064 138 521 2274 374 146 1280 Fragments arranged by size: 2274 1280 1064 521 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3860 4457 5244 5512 1239 Size: 393 2228 597 787 268 1524 Fragments arranged by size: 2228 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2081 2153 4944 1678 Size: 403 72 2791 2531 Fragments arranged by size: 2791 2531 403 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373 Size: 32 135 32 2866 32 42 32 2626 Fragments arranged by size: 2866 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 3141 3387 669 Size: 988 281 358 845 246 3079 Fragments arranged by size: 3079 988 845 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2077 2077 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 10 Size: 54 1425 343 489 3486 Fragments arranged by size: 3486 1425 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 3011 3755 4114 4273 518 Size: 648 921 924 744 359 159 2042 Fragments arranged by size: 2042 924 921 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3213 3890 5038 5283 1606 Size: 1607 677 1148 245 2120 Fragments arranged by size: 2120 1607 1148 677 245 NarI GG'CG_CC Cuts at: 2078 2078 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 2832 387 Size: 2445 3352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2348 2612 3010 3155 4700 1132 Size: 97 1119 264 398 145 1545 2229 Fragments arranged by size: 2229 1545 1119 398 264 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 2344 2608 4696 2344 Size: 264 2088 3445 Fragments arranged by size: 3445 2088 264 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 2185 2390 1108 Size: 1044 33 205 4515 Fragments arranged by size: 4515 1044 205 33 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PstI C_TGCA'G Cuts at: 830 3004 3149 830 Size: 2174 145 3478 Fragments arranged by size: 3478 2174 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SapI GCTCTTCn'nnn_ Cuts at: 2198 2198 Size: 5797 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 3000 3145 4280 4961 5152 826 Size: 219 1955 145 1135 681 191 1471 Fragments arranged by size: 1955 1471 1135 681 219 191 145 SfoI GGC'GCC Cuts at: 2079 2079 Size: 5797 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 3901 4802 5064 820 Size: 197 926 1043 915 901 262 1553 Fragments arranged by size: 1553 1043 926 915 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3010 3155 1132 Size: 1878 145 3774 Fragments arranged by size: 3774 1878 145 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 3236 513 Size: 552 2171 3074 Fragments arranged by size: 3074 2171 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2382 3032 3824 3841 3994 4179 4624 Size: 1255 802 650 792 17 153 185 445 Cuts at: 4624 325 Size: 1498 Fragments arranged by size: 1498 1255 802 792 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 5757 5789 2018 Size: 32 3707 32 2026 Fragments arranged by size: 3707 2026 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2089 2089 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI