Restriction map for
pFN21AE0321
(Circular) (Six-base) MAPSORT of: pFN21AE0321.seq Check: 9867 from: 1 to: 7363
pFN21AE0321 7363 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3664 3664
Size: 7363
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5173 278
Size: 53 83 186 936 3637 2468
Fragments arranged by size:
3637 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4219 4706 1937
Size: 2282 487 4594
Fragments arranged by size:
4594 2282 487
Acc65I G'GTAC_C
Cuts at: 4684 4684
Size: 7363
AclI AA'CG_TT
Cuts at: 1563 5490 5863 1563
Size: 3927 373 3063
Fragments arranged by size:
3927 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2218 2972 3183 4401 5426 6810 7078 1632
Size: 586 754 211 1218 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1218 1025 754 586 268 211
AfeI AGC'GCT
Cuts at: 2525 2525
Size: 7363
AflII C'TTAA_G
Cuts at: 820 1017 2743 820
Size: 197 1726 5440
Fragments arranged by size:
5440 1726 197
AflIII A'CryG_T
Cuts at: 1203 2169 3790 6262 7239 1203
Size: 966 1621 2472 977 1327
Fragments arranged by size:
2472 1621 1327 977 966
AgeI A'CCGG_T
Cuts at: 6194 6194
Size: 7363
AhdI GACnn_n'nnGTC
Cuts at: 6092 6092
Size: 7363
AleI CACnn'nnGTG
Cuts at: 1166 3368 1166
Size: 2202 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3327 1184
Size: 2143 5220
AlwNI CAG_nnn'CTG
Cuts at: 2493 2503 2950 3066 3254 4132 4289 5106 6678
Size: 10 447 116 188 878 157 817 1572
Cuts at: 6678 2493
Size: 3178
Fragments arranged by size:
3178 1572 878 817 447 188 157 116
10
ApaI G_GGCC'C
Cuts at: 2836 4085 2836
Size: 1249 6114
ApaLI G'TGCA_C
Cuts at: 3017 5420 6576 3017
Size: 2403 1156 3804
Fragments arranged by size:
3804 2403 1156
ApoI r'AATT_y
Cuts at: 1493 2201 3406 3973 3991 4672 4898 5050 7159
Size: 708 1205 567 18 681 226 152 2109
Cuts at: 7159 1493
Size: 1697
Fragments arranged by size:
2109 1697 1205 708 681 567 226 152
18
AseI AT'TA_AT
Cuts at: 160 1032 5917 160
Size: 872 4885 1606
Fragments arranged by size:
4885 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 3462 4656 4688 1870
Size: 73 886 633 1194 32 4545
Fragments arranged by size:
4545 1194 886 633 73 32
BamHI G'GATC_C
Cuts at: 4472 4693 5060 4472
Size: 221 367 6775
Fragments arranged by size:
6775 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4684 6139 618
Size: 325 217 627 2897 1455 1842
Fragments arranged by size:
2897 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2589 2670 2836 4049 4085 4098 4214 4330
Size: 1198 81 166 1213 36 13 116 116
Cuts at: 4330 4385 4682 1391
Size: 55 297 4072
Fragments arranged by size:
4072 1213 1198 297 166 116 116 81
55 36 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2779 928
Size: 971 880 5512
Fragments arranged by size:
5512 971 880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5554 5588 1903
Size: 34 3617 34 3678
Fragments arranged by size:
3678 3617 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2557 5255 6465 2557
Size: 2698 1210 3455
Fragments arranged by size:
3455 2698 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7363
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2350 4030 4516
Size: 107 122 71 631 605 678 1680 486
Cuts at: 4516 5974 136
Size: 1458 1525
Fragments arranged by size:
1680 1525 1458 678 631 605 486 122
107 71
BglII A'GATC_T
Cuts at: 2620 7358 2620
Size: 4738 2625
BlpI GC'TnA_GC
Cuts at: 4780 4780
Size: 7363
Bme1580I G_kGCm'C
Cuts at: 1790 2836 3021 3141 3922 4085 5424 6580 1790
Size: 1046 185 120 781 163 1339 1156 2573
Fragments arranged by size:
2573 1339 1156 1046 781 185 163 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 5094 6052
Size: 416 53 83 399 24 1637 2040 958
Cuts at: 6052 442
Size: 1753
Fragments arranged by size:
2040 1753 1637 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3119 3631 4293 4344 4484 6023 1239
Size: 1880 512 662 51 140 1539 2579
Fragments arranged by size:
2579 1880 1539 662 512 140 51
Bpu10I CC'TnA_GC
Cuts at: 3812 3812
Size: 7363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 5488 6353 6651 3931
Size: 1557 865 298 4643
Fragments arranged by size:
4643 1557 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2367 3459 6026 882
Size: 617 868 1092 2567 2219
Fragments arranged by size:
2567 2219 1092 868 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 2924 5154 493
Size: 711 864 856 2230 2702
Fragments arranged by size:
2702 2230 864 856 711
BsaBI GATnn'nnATC
Cuts at: 7357 7357
Size: 7363
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2120 5170 5552 6187
Size: 53 83 186 936 587 3050 382 635
Cuts at: 6187 275
Size: 1451
Fragments arranged by size:
3050 1451 936 635 587 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2530 5796 6194 6468 6615 7026 2530
Size: 3266 398 274 147 411 2867
Fragments arranged by size:
3266 2867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 4636 4666 1178
Size: 30 165 30 1062 30 2141 30 3875
Fragments arranged by size:
3875 2141 1062 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4186 1186
Size: 3000 4363
BseYI C'CCAG_C
Cuts at: 2358 3998 4078 4168 4189 4207 4468 6566 7062
Size: 1640 80 90 21 18 261 2098 496
Cuts at: 7062 2358
Size: 2659
Fragments arranged by size:
2659 2098 1640 496 261 90 80 21
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 1163
Size: 573 1218 5572
Fragments arranged by size:
5572 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4813 5574 6602 1540
Size: 275 186 1501 1311 761 1028 2301
Fragments arranged by size:
2301 1501 1311 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2589 3021 3054 3365 3753 4330 5424 5509 6580
Size: 432 33 311 388 577 1094 85 1071
Cuts at: 6580 2589
Size: 3372
Fragments arranged by size:
3372 1094 1071 577 432 388 311 85
33
BsmI GAATG_Cn'
Cuts at: 2256 2367 4874 4967 2256
Size: 111 2507 93 4652
Fragments arranged by size:
4652 2507 111 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2393 4108 2393
Size: 1715 5648
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2589 2670 2836 3021 3054 3141 3365
Size: 399 799 81 166 185 33 87 224
Cuts at: 3365 3753 3922 4049 4085 4098 4214 4330 4385
Size: 388 169 127 36 13 116 116 55
Cuts at: 4385 4682 5424 5509 6580 1391
Size: 297 742 85 1071 2174
Fragments arranged by size:
2174 1071 799 742 399 388 297 224
185 169 166 127 116 116 87 85
81 55 36 33 13
BspEI T'CCGG_A
Cuts at: 2530 7026 2530
Size: 4496 2867
BspHI T'CATG_A
Cuts at: 5252 6982 5252
Size: 1730 5633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3309 3664 4718 844
Size: 2465 355 1054 3489
Fragments arranged by size:
3489 2465 1054 355
BsrBI CCG'CTC
Cuts at: 1394 1823 4524 5250 1394
Size: 429 2701 726 3507
Fragments arranged by size:
3507 2701 726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 3693 5858 6032 65
Size: 1183 891 1554 2165 174 1396
Fragments arranged by size:
2165 1554 1396 1183 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2376 3634 3935 3947 6007 6194 1700
Size: 110 566 1258 301 12 2060 187 2869
Fragments arranged by size:
2869 2060 1258 566 301 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7363
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7363
BssSI C'ACGA_G
Cuts at: 5423 6435 5423
Size: 1012 6351
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7363
BstBI TT'CG_AA
Cuts at: 3856 6167 3856
Size: 2311 5052
BstEII G'GTnAC_C
Cuts at: 1184 4063 1184
Size: 2879 4484
BstXI CCAn_nnnn'nTGG
Cuts at: 3608 3608
Size: 7363
BstYI r'GATC_y
Cuts at: 1972 2620 4472 4693 5060 5445 5462 6914 7000
Size: 648 1852 221 367 385 17 1452 86
Cuts at: 7000 7012 7358 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1852 1452 648 385 367 346 221
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3523 4500 4611 3523
Size: 977 111 6275
Fragments arranged by size:
6275 977 111
BtgI C'CryG_G
Cuts at: 513 1065 2004 2443 2644 3725 4514 513
Size: 552 939 439 201 1081 789 3362
Fragments arranged by size:
3362 1081 939 789 552 439 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6624
Fragments arranged by size:
6624 683 56
BtsI GCAGTG_nn'
Cuts at: 2339 4462 4879 5673 5693 2339
Size: 2123 417 794 20 4009
Fragments arranged by size:
4009 2123 794 417 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7363
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 529
Size: 35 2695 35 4598
Fragments arranged by size:
4598 2695 35 35
DraI TTT'AAA
Cuts at: 4667 4667
Size: 7363
DraIII CAC_nnn'GTG
Cuts at: 3016 4075 3016
Size: 1059 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6370 809
Size: 507 5054 1802
Fragments arranged by size:
5054 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2351 2976 3478
Size: 54 1425 50 275 18 521 625 502
Cuts at: 3478 3499 3690 4810 5699 7328 8
Size: 21 191 1120 889 1629 43
Fragments arranged by size:
1629 1425 1120 889 625 521 502 275
191 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 4810 1537
Size: 275 1687 1311 4090
Fragments arranged by size:
4090 1687 1311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 2858 3237 3839 4420 5293 1353
Size: 799 706 379 602 581 873 3423
Fragments arranged by size:
3423 873 799 706 602 581 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3728 5950 6324 6470 387
Size: 1064 138 2139 2222 374 146 1280
Fragments arranged by size:
2222 2139 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2587 4328 2587
Size: 1741 5622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 2972 3119 3183 3631 4293 4344
Size: 393 586 754 147 64 448 662 51
Cuts at: 4344 4401 4484 5426 6023 6810 7078 1239
Size: 57 83 942 597 787 268 1524
Fragments arranged by size:
1524 942 787 754 662 597 586 448
393 268 147 83 64 57 51
EcoO109I rG'GnC_Cy
Cuts at: 3850 4082 4421 4509 4595 3850
Size: 232 339 88 86 6618
Fragments arranged by size:
6618 339 232 88 86
EcoRI G'AATT_C
Cuts at: 3406 3991 4672 5050 3406
Size: 585 681 378 5719
Fragments arranged by size:
5719 681 585 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3017 3017
Size: 7363
FspI TGC'GCA
Cuts at: 2336 5869 2336
Size: 3533 3830
FspAI rTGC'GCAy
Cuts at: 2336 2336
Size: 7363
HaeII r_GCGC'y
Cuts at: 1678 2527 6510 1678
Size: 849 3983 2531
Fragments arranged by size:
3983 2531 849
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2759 2791 3467
Size: 32 135 32 575 32 580 32 676
Cuts at: 3467 3499 4312 4344 4457 4489 4556 4588 4636
Size: 32 813 32 113 32 67 32 48
Cuts at: 4636 4668 6004 6036 6078 6110 1373
Size: 32 1336 32 42 32 2626
Fragments arranged by size:
2626 1336 813 676 580 575 135 113
67 48 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3838 4707 4953 669
Size: 988 281 1900 869 246 3079
Fragments arranged by size:
3079 1900 988 869 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7363
HpaI GTT'AAC
Cuts at: 4953 4953
Size: 7363
KpnI G_GTAC'C
Cuts at: 4688 4688
Size: 7363
MfeI C'AATT_G
Cuts at: 4962 4962
Size: 7363
MluI A'CGCG_T
Cuts at: 7239 7239
Size: 7363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 2839 6477 6661 1305
Size: 885 649 3638 184 2007
Fragments arranged by size:
3638 2007 885 649 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3480 3692 10
Size: 54 1425 343 1648 212 3681
Fragments arranged by size:
3681 1648 1425 343 212 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3309 3368 3550 3606 5321 5680 5839
Size: 648 2143 59 182 56 1715 359 159
Cuts at: 5839 518
Size: 2042
Fragments arranged by size:
2143 2042 1715 648 359 182 159 59
56
MspA1I CmG'CkG
Cuts at: 1606 3146 4779 5456 6604 6849 1606
Size: 1540 1633 677 1148 245 2120
Fragments arranged by size:
2120 1633 1540 1148 677 245
NaeI GCC'GGC
Cuts at: 3636 3636
Size: 7363
NcoI C'CATG_G
Cuts at: 513 1065 2004 2644 513
Size: 552 939 640 5232
Fragments arranged by size:
5232 939 640 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7363
NgoMIV G'CCGG_C
Cuts at: 3634 3634
Size: 7363
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7363
NotI GC'GGCC_GC
Cuts at: 4810 4810
Size: 7363
NspI r_CATG'y
Cuts at: 1132 1229 3314 3382 3794 4721 6266 1132
Size: 97 2085 68 412 927 1545 2229
Fragments arranged by size:
2229 2085 1545 927 412 97 68
PasI CC'CwG_GG
Cuts at: 1348 2314 2762 1348
Size: 966 448 5949
Fragments arranged by size:
5949 966 448
PciI A'CATG_T
Cuts at: 3790 6262 3790
Size: 2472 4891
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2172 2320 1108
Size: 1064 148 6151
Fragments arranged by size:
6151 1064 148
PfoI T'CCnGG_A
Cuts at: 4574 4574
Size: 7363
PmeI GTTT'AAAC
Cuts at: 4667 4667
Size: 7363
PmlI CAC'GTG
Cuts at: 2068 2924 2068
Size: 856 6507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3183 3215 5432 5464 6945 6977 3183
Size: 32 2217 32 1481 32 3569
Fragments arranged by size:
3569 2217 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4595 4595
Size: 7363
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7363
PsiI TTA'TAA
Cuts at: 4933 4933
Size: 7363
PspOMI G'GGCC_C
Cuts at: 2832 4081 2832
Size: 1249 6114
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 7331
PstI C_TGCA'G
Cuts at: 830 4030 4150 4715 830
Size: 3200 120 565 3478
Fragments arranged by size:
3478 3200 565 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7363
PvuII CAG'CTG
Cuts at: 3146 3146
Size: 7363
SacI G_AGCT'C
Cuts at: 2589 4330 2589
Size: 1741 5622
SalI G'TCGA_C
Cuts at: 1936 4705 1936
Size: 2769 4594
SanDI GG'GwC_CC
Cuts at: 4595 4595
Size: 7363
SapI GCTCTTCn'nnn_
Cuts at: 4420 4420
Size: 7363
SbfI CC_TGCA'GG
Cuts at: 4030 4150 4715 4030
Size: 120 565 6678
Fragments arranged by size:
6678 565 120
ScaI AGT'ACT
Cuts at: 5611 7323 5611
Size: 1712 5651
SfcI C'TryA_G
Cuts at: 826 1045 2485 4026 4146 4552 4711 5846 6527
Size: 219 1440 1541 120 406 159 1135 681
Cuts at: 6527 6718 826
Size: 191 1471
Fragments arranged by size:
1541 1471 1440 1135 681 406 219 191
159 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 4516 4516
Size: 7363
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7363
SmaI CCC'GGG
Cuts at: 1872 2831 4658 4690 1872
Size: 959 1827 32 4545
Fragments arranged by size:
4545 1827 959 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2743 3910 5467 6368 6630 820
Size: 197 926 800 1167 1557 901 262 1553
Fragments arranged by size:
1557 1553 1167 926 901 800 262 197
SnaBI TAC'GTA
Cuts at: 493 5154 493
Size: 4661 2702
SpeI A'CTAG_T
Cuts at: 152 4178 152
Size: 4026 3337
SphI G_CATG'C
Cuts at: 1132 4721 1132
Size: 3589 3774
SspI AAT'ATT
Cuts at: 5 52 5287 5
Size: 47 5235 2081
Fragments arranged by size:
5235 2081 47
StuI AGG'CCT
Cuts at: 1517 3810 4041 1517
Size: 2293 231 4839
Fragments arranged by size:
4839 2293 231
StyI C'CwwG_G
Cuts at: 513 1065 2004 2644 4599 4802 513
Size: 552 939 640 1955 203 3074
Fragments arranged by size:
3074 1955 939 640 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2775 3380 3821 3880 4082 5390 5407
Size: 1255 1195 605 441 59 202 1308 17
Cuts at: 5407 5560 5745 6190 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1308 1255 1195 605 445 441 202
185 153 59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2755 3739 5609
Size: 274 80 33 51 1443 778 984 1870
Cuts at: 5609 7321 96
Size: 1712 138
Fragments arranged by size:
1870 1712 1443 984 778 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7323 7355 7323
Size: 32 7331
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7363
XbaI T'CTAG_A
Cuts at: 2674 4699 2674
Size: 2025 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3655 3700 3655
Size: 45 7318
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7363
XmaI C'CCGG_G
Cuts at: 1870 2829 4656 4688 1870
Size: 959 1827 32 4545
Fragments arranged by size:
4545 1827 959 32
XmnI GAAnn'nnTTC
Cuts at: 2367 5492 2367
Size: 3125 4238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5171 276
Size: 53 83 186 936 3637 2468
Fragments arranged by size:
3637 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoNI EcoRV FseI KasI NarI NruI
NsiI PacI PspXI RsrII SacII SexAI SfoI SrfI
SwaI