(Circular) (Six-base) MAPSORT of: pFN21AB8998.seq Check: 2836 from: 1 to: 5941 pFN21AB8998 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2266 2648 4004 5388 5656 1632 Size: 634 382 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 634 382 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2650 3684 5256 2650 Size: 1034 1572 3335 Fragments arranged by size: 3335 1572 1034 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2967 3266 1870 Size: 73 1024 299 4545 Fragments arranged by size: 4545 1024 299 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2566 2957 3262 4717 618 Size: 325 217 627 779 391 305 1455 1842 Fragments arranged by size: 1842 1455 779 627 391 325 305 217 BanII G_rGCy'C Cuts at: 1391 2021 2276 2727 3159 3174 3195 3260 1391 Size: 630 255 451 432 15 21 65 4072 Fragments arranged by size: 4072 630 451 432 255 65 21 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4132 4166 1903 Size: 34 50 34 2111 34 3678 Fragments arranged by size: 3678 2111 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2888 3833 5043 2888 Size: 945 1210 3786 Fragments arranged by size: 3786 1210 945 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2571 2668 2968 3118 3188 4002 5158 1790 Size: 781 97 300 150 70 814 1156 2573 Fragments arranged by size: 2573 1156 814 781 300 150 97 70 BmgBI CAC'GTC Cuts at: 1318 2923 3151 1318 Size: 1605 228 4108 Fragments arranged by size: 4108 1605 228 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2664 2664 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4601 1239 Size: 3362 2579 Bpu10I CC'TnA_GC Cuts at: 2367 3066 3161 2367 Size: 699 95 5147 Fragments arranged by size: 5147 699 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3158 4066 4931 5229 3158 Size: 908 865 298 3870 Fragments arranged by size: 3870 908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4374 4772 5046 5193 5604 2334 Size: 2040 398 274 147 411 2671 Fragments arranged by size: 2671 2040 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2988 3018 3219 3225 3249 Size: 30 165 30 1585 30 201 6 24 Cuts at: 3249 3255 1178 Size: 6 3864 Fragments arranged by size: 3864 1585 201 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2011 1186 Size: 825 5116 BseYI C'CCAG_C Cuts at: 2088 2388 2584 5144 5640 2088 Size: 300 196 2560 496 2389 Fragments arranged by size: 2560 2389 496 300 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 3391 4152 5180 1540 Size: 275 186 156 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2178 2276 3058 3159 3174 4002 4087 5158 2178 Size: 98 782 101 15 828 85 1071 2961 Fragments arranged by size: 2961 1071 828 782 101 98 85 15 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 3020 3020 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2178 2276 2571 2668 2727 2968 Size: 399 231 157 98 295 97 59 241 Cuts at: 2968 3058 3118 3159 3174 3188 3195 3260 4002 Size: 90 60 41 15 14 7 65 742 Cuts at: 4002 4087 5158 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 241 231 157 98 97 90 85 65 60 59 41 15 14 7 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2992 3296 844 Size: 1277 871 304 3489 Fragments arranged by size: 3489 1277 871 304 BsrBI CCG'CTC Cuts at: 1394 1823 2332 2662 3070 3828 1394 Size: 429 509 330 408 758 3507 Fragments arranged by size: 3507 758 509 429 408 330 BsrDI GCAATG_nn' Cuts at: 65 1248 2049 2080 2241 2313 2376 4436 4610 Size: 1183 801 31 161 72 63 2060 174 Cuts at: 4610 65 Size: 1396 Fragments arranged by size: 2060 1396 1183 801 174 161 72 63 31 BsrFI r'CCGG_y Cuts at: 1700 1810 2354 4585 4772 1700 Size: 110 544 2231 187 2869 Fragments arranged by size: 2869 2231 544 187 110 BsrGI T'GTAC_A Cuts at: 96 2028 96 Size: 1932 4009 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2359 4001 5013 2359 Size: 1642 1012 3287 Fragments arranged by size: 3287 1642 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 2350 1184 Size: 1166 4775 BstXI CCAn_nnnn'nTGG Cuts at: 2707 2719 3039 2707 Size: 12 320 5609 Fragments arranged by size: 5609 320 12 BstYI r'GATC_y Cuts at: 1972 2601 3271 3638 4023 4040 5492 5578 5590 Size: 629 670 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 670 629 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2927 2927 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2227 513 Size: 552 1162 4227 Fragments arranged by size: 4227 1162 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2146 2281 487 Size: 683 56 920 135 4147 Fragments arranged by size: 4147 920 683 135 56 BtsI GCAGTG_nn' Cuts at: 2172 2675 2995 3058 3457 4251 4271 2172 Size: 503 320 63 399 794 20 3842 Fragments arranged by size: 3842 794 503 399 320 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2061 2154 Size: 54 1425 50 275 18 216 15 93 Cuts at: 2154 2292 2709 2820 3022 3388 4277 5906 8 Size: 138 417 111 202 366 889 1629 43 Fragments arranged by size: 1629 1425 889 417 366 275 216 202 138 111 93 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2154 3388 1537 Size: 275 342 1234 4090 Fragments arranged by size: 4090 1234 342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 3871 1353 Size: 890 1628 3423 Fragments arranged by size: 3423 1628 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2879 4528 4902 5048 387 Size: 1064 138 1290 1649 374 146 1280 Fragments arranged by size: 1649 1290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2274 3157 3172 2274 Size: 883 15 5043 Fragments arranged by size: 5043 883 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2266 2648 4004 4601 5388 5656 1239 Size: 393 634 382 1356 597 787 268 1524 Fragments arranged by size: 1524 1356 787 634 597 393 382 268 EcoNI CCTnn'n_nnAGG Cuts at: 3218 3218 Size: 5941 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2988 3020 4582 4614 4656 Size: 32 135 32 1416 32 1562 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1562 1416 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2411 5055 5239 1305 Size: 1106 2644 184 2007 Fragments arranged by size: 2644 2007 1106 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 2063 2294 2711 2822 Size: 54 1425 343 216 15 231 417 111 Cuts at: 2822 10 Size: 3129 Fragments arranged by size: 3129 1425 417 343 231 216 111 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2054 2165 2717 2988 3899 4258 Size: 648 870 18 111 552 271 911 359 Cuts at: 4258 4417 518 Size: 159 2042 Fragments arranged by size: 2042 911 870 648 552 359 271 159 111 18 MspA1I CmG'CkG Cuts at: 1606 2626 2954 3357 4034 5182 5427 1606 Size: 1020 328 403 677 1148 245 2120 Fragments arranged by size: 2120 1148 1020 677 403 328 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2473 3091 3127 1348 Size: 1125 618 36 4162 Fragments arranged by size: 4162 1125 618 36 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2388 1108 Size: 1280 4661 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PstI C_TGCA'G Cuts at: 830 2118 2682 2792 3293 830 Size: 1288 564 110 501 3478 Fragments arranged by size: 3478 1288 564 501 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2626 2954 2626 Size: 328 5613 SacI G_AGCT'C Cuts at: 2276 3159 3174 2276 Size: 883 15 5043 Fragments arranged by size: 5043 883 15 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2114 2678 2788 3289 4424 5105 5296 Size: 219 1069 564 110 501 1135 681 191 Cuts at: 5296 826 Size: 1471 Fragments arranged by size: 1471 1135 1069 681 564 501 219 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2969 3268 1872 Size: 1097 299 4545 Fragments arranged by size: 4545 1097 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 3173 4045 4946 5208 820 Size: 197 926 1230 872 901 262 1553 Fragments arranged by size: 1553 1230 926 901 872 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 3380 513 Size: 552 2315 3074 Fragments arranged by size: 3074 2315 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2380 2576 3968 3985 4138 4323 Size: 1255 579 221 196 1392 17 153 185 Cuts at: 4323 4768 325 Size: 445 1498 Fragments arranged by size: 1498 1392 1255 579 445 221 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 2538 4187 Size: 274 80 33 51 1443 51 510 1649 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1649 1443 510 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3219 3251 5901 5933 3219 Size: 32 2650 32 3227 Fragments arranged by size: 3227 2650 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2056 2134 2530 2011 Size: 45 78 396 5422 Fragments arranged by size: 5422 396 78 45 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2967 3266 1870 Size: 1097 299 4545 Fragments arranged by size: 4545 1097 299 XmnI GAAnn'nnTTC Cuts at: 2858 4070 2858 Size: 1212 4729 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI