Restriction map for
pFN21AB6865
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DdeI
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI MaeI MfeI MluI
MluNI MscI MslI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 344 TCCGGA T^CCGGA
AcsI 517 AAATTT [GA]^AATT[CT]
AcyI 25 GACGCC G[GA]^CG[CT]C
AgeI 456 ACCGGT A^CCGGT
ApoI 517 AAATTT [GA]^AATT[CT]
AspHI 106 GTGCTC G[AT]GC[AT]^C
AvaII 333 GGACC G^G[AT]CC
BamHI 341 GGATCC G^GATCC
BcgI 428 CGAAGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 71 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 106 GTGCTC G[AGT]GC[ACT]^C
BpmI 82 CTGGAG CTGGAG(16/14)
BsaAI 511 TACGTG [CT]AC^GT[GA]
BsaHI 25 GACGCC G[GA]^CG[CT]C
BseAI 344 TCCGGA T^CCGGA
BseRI 256 GAGGAG GAGGAG(10/8)
BsgI 4 GTGCAG GTGCAG(16/14)
BsiEI 66 CGGCCG CG[GA][CT]^CG
BsiHKAI 106 GTGCTC G[AT]GC[AT]^C
BsmAI 365 GAGAC GTCTC(1/5)
BsmFI 323 GGGAC GGGAC(10/14)
Bsp1286I 106 GTGCTC G[AGT]GC[ACT]^C
BspEI 344 TCCGGA T^CCGGA
EaeI 66 CGGCCG [CT]^GGCC[GA]
EagI 66 CGGCCG C^GGCCG
EarI 167 GAAGAG CTCTTC(1/4)
EclXI 66 CGGCCG C^GGCCG
Eco52I 66 CGGCCG C^GGCCG
Hin1I 25 GACGCC G[GA]^CG[CT]C
Ksp632I 167 GAAGAG CTCTTC(1/4)
MaeIII 373 GTGAC ^GT[ACGT]AC
MroI 344 TCCGGA T^CCGGA
MseI 242 TTAA T^TAA
NdeI 204 CATATG CA^TATG
NlaIV 341 GGATCC GG[ACGT]^[ACGT]CC
PinAI 456 ACCGGT A^CCGGT
PvuII 88 CAGCTG CAG^CTG
SfaNI 124 GATGC GCATC(5/9)
SmaI 30 CCCGGG CCC^GGG
StuI 8 AGGCCT AGG^CCT
TaqI 314 TCGA T^CGA
Tru9I 242 TTAA T^TAA
Tsp45I 373 GTGAC ^GT[GC]AC
TthHB8I 314 TCGA T^CGA
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 89 AGCT AG^CT
119 AGCT
AvaI 30 CCCGGG C^[CT]CG[GA]G
100 CTCGGG
BbvI 87 GCAGC GCAGC(8/12)
90 GCTGC
BsaBI 189 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
342 GATCCGGATC
BsaWI 344 TCCGGA [AT]^CCGG[AT]
456 ACCGGT
BsoBI 30 CCCGGG C^[CT]CG[GA]G
100 CTCGGG
BsrFI 456 ACCGGT [GA]^CCGG[CT]
476 ACCGGC
Cfr10I 456 ACCGGT [GA]^CCGG[CT]
476 ACCGGC
HgaI 25 GACGC GACGC(5/10)
375 GACGC
HphI 372 GGTGA GGTGA(8/7)
396 TCACC
MaeII 317 ACGT A^CGT
512 ACGT
MamI 189 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
342 GATCCGGATC
TseI 87 GCAGC G^C[AT]GC
90 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AfaI AlwI BcnI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI KspI MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII RsaI
SacII Sau3AI Sau96I ScrFI Tsp509I
TspRI XhoII