Restriction map for
pFN21AB6865
(Linear) (Six-base) MAPSORT of: KIBB6865.seq Check: 439 from: 1 to: 537
KIBB6865.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AgeI A'CCGG_T
Cuts at: 0 456 537
Size: 456 81
ApoI r'AATT_y
Cuts at: 0 517 537
Size: 517 20
AvaI C'yCGr_G
Cuts at: 0 30 100 537
Size: 30 70 437
Fragments arranged by size:
437 70 30
BamHI G'GATC_C
Cuts at: 0 341 537
Size: 341 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 417 451 537
Size: 417 34 86
Fragments arranged by size:
417 86 34
BglI GCCn_nnn'nGGC
Cuts at: 0 77 537
Size: 77 460
BmgBI CAC'GTC
Cuts at: 0 318 537
Size: 318 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 537
Size: 67 470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 537
Size: 103 434
BsaAI yAC'GTr
Cuts at: 0 513 537
Size: 513 24
BsaBI GATnn'nnATC
Cuts at: 0 193 346 537
Size: 193 153 191
Fragments arranged by size:
193 191 153
BsaHI Gr'CG_yC
Cuts at: 0 26 537
Size: 26 511
BsaWI w'CCGG_w
Cuts at: 0 344 456 537
Size: 344 112 81
Fragments arranged by size:
344 112 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 356 386 443 473 537
Size: 322 30 4 30 57 30 64
Fragments arranged by size:
322 64 57 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 537
Size: 271 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 537
Size: 25 512
BsiEI CG_ry'CG
Cuts at: 0 69 537
Size: 69 468
BsiHKAI G_wGCw'C
Cuts at: 0 110 537
Size: 110 427
Bsp1286I G_dGCh'C
Cuts at: 0 110 537
Size: 110 427
BspEI T'CCGG_A
Cuts at: 0 344 537
Size: 344 193
BsrFI r'CCGG_y
Cuts at: 0 456 476 537
Size: 456 20 61
Fragments arranged by size:
456 61 20
BstYI r'GATC_y
Cuts at: 0 161 281 341 347 389 537
Size: 161 120 60 6 42 148
Fragments arranged by size:
161 148 120 60 42 6
BtgI C'CryG_G
Cuts at: 0 40 383 501 537
Size: 40 343 118 36
Fragments arranged by size:
343 118 40 36
BtsI GCAGTG_nn'
Cuts at: 0 309 537
Size: 309 228
EaeI y'GGCC_r
Cuts at: 0 66 537
Size: 66 471
EagI C'GGCC_G
Cuts at: 0 66 537
Size: 66 471
EarI CTCTTCn'nnn_
Cuts at: 0 162 537
Size: 162 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 537
Size: 103 434
MspA1I CmG'CkG
Cuts at: 0 42 90 385 484 503 537
Size: 42 48 295 99 19 34
Fragments arranged by size:
295 99 48 42 34 19
NdeI CA'TA_TG
Cuts at: 0 205 537
Size: 205 332
PfoI T'CCnGG_A
Cuts at: 0 184 293 537
Size: 184 109 244
Fragments arranged by size:
244 184 109
PvuII CAG'CTG
Cuts at: 0 90 537
Size: 90 447
SacII CC_GC'GG
Cuts at: 0 43 386 504 537
Size: 43 343 118 33
Fragments arranged by size:
343 118 43 33
SmaI CCC'GGG
Cuts at: 0 32 537
Size: 32 505
StuI AGG'CCT
Cuts at: 0 10 537
Size: 10 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 356 388 443 475 537
Size: 322 32 2 32 55 32 62
Fragments arranged by size:
322 62 55 32 32 32 2
XmaI C'CCGG_G
Cuts at: 0 30 537
Size: 30 507
Enzymes that do cut:
AgeI ApoI AvaI BamHI BcgI BglI BmgBI BmrI
BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstYI BtgI BtsI
EaeI EagI EarI Eco57MI MspA1I NdeI PfoI PvuII
SacII SmaI StuI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmtI BplI Bpu10I BpuEI BsaI BseYI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmnI ZraI