(Circular) (Six-base) MAPSORT of: pFN21AE0358.seq Check: 7452 from: 1 to: 6967 pFN21AE0358 6967 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4777 278 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4310 1937 Size: 2373 4594 Acc65I G'GTAC_C Cuts at: 4288 4288 Size: 6967 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2386 2583 3048 3332 5030 6414 6682 1632 Size: 754 197 465 284 1698 1384 268 1917 Fragments arranged by size: 1917 1698 1384 754 465 284 268 197 AflII C'TTAA_G Cuts at: 820 1017 3737 820 Size: 197 2720 4050 Fragments arranged by size: 4050 2720 197 AflIII A'CryG_T Cuts at: 1203 2378 2899 3302 3997 5866 6843 1203 Size: 1175 521 403 695 1869 977 1327 Fragments arranged by size: 1869 1327 1175 977 695 521 403 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 5696 5696 Size: 6967 AleI CACnn'nnGTG Cuts at: 1166 3303 1166 Size: 2137 4830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6967 AlwNI CAG_nnn'CTG Cuts at: 4710 6282 4710 Size: 1572 5395 ApaLI G'TGCA_C Cuts at: 3421 5024 6180 3421 Size: 1603 1156 4208 Fragments arranged by size: 4208 1603 1156 ApoI r'AATT_y Cuts at: 1493 2679 2910 3110 3608 4197 4276 4502 4654 Size: 1186 231 200 498 589 79 226 152 Cuts at: 4654 6763 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1186 589 498 231 226 200 152 79 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 2012 4292 1870 Size: 73 69 2280 4545 Fragments arranged by size: 4545 2280 73 69 AvrII C'CTAG_G Cuts at: 2245 2245 Size: 6967 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4141 4174 4141 Size: 33 6934 BamHI G'GATC_C Cuts at: 2336 3742 4297 4664 2336 Size: 1406 555 367 4639 Fragments arranged by size: 4639 1406 555 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 2498 3970 4288 5743 Size: 325 217 627 701 10 1472 318 1455 Cuts at: 5743 618 Size: 1842 Fragments arranged by size: 1842 1472 1455 701 627 325 318 217 10 BanII G_rGCy'C Cuts at: 1391 4115 4286 1391 Size: 2724 171 4072 Fragments arranged by size: 4072 2724 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2574 3726 928 Size: 971 675 1152 4169 Fragments arranged by size: 4169 1152 971 675 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5158 5192 1903 Size: 34 50 34 3137 34 3678 Fragments arranged by size: 3678 3137 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4142 4859 6069 3608 Size: 534 717 1210 4506 Fragments arranged by size: 4506 1210 717 534 BclI T'GATC_A Cuts at: 1548 2860 1548 Size: 1312 5655 BfrBI ATG'CAT Cuts at: 3171 3171 Size: 6967 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5578 136 Size: 107 122 71 631 605 3906 1525 Fragments arranged by size: 3906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3678 6962 3678 Size: 3284 3683 BlpI GC'TnA_GC Cuts at: 4384 4384 Size: 6967 Bme1580I G_kGCm'C Cuts at: 1790 3425 5028 6184 1790 Size: 1635 1603 1156 2573 Fragments arranged by size: 2573 1635 1603 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3023 4698 5656 Size: 416 53 83 399 24 1606 1675 958 Cuts at: 5656 442 Size: 1753 Fragments arranged by size: 1753 1675 1606 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6967 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3721 3721 Size: 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3711 3929 5627 1239 Size: 2472 218 1698 2579 Fragments arranged by size: 2579 2472 1698 218 Bpu10I CC'TnA_GC Cuts at: 3267 3267 Size: 6967 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5092 5957 6255 5092 Size: 865 298 5804 Fragments arranged by size: 5804 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5630 882 Size: 617 4131 2219 Fragments arranged by size: 4131 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2379 4758 493 Size: 711 1175 2379 2702 Fragments arranged by size: 2702 2379 1175 711 BsaBI GATnn'nnATC Cuts at: 2859 4251 6961 2859 Size: 1392 2710 2865 Fragments arranged by size: 2865 2710 1392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3818 4774 5156 5791 Size: 53 83 186 936 2285 956 382 635 Cuts at: 5791 275 Size: 1451 Fragments arranged by size: 2285 1451 956 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 5798 6072 6219 6630 5400 Size: 398 274 147 411 5737 Fragments arranged by size: 5737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6742 Fragments arranged by size: 6742 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3668 1186 Size: 2482 4485 BseYI C'CCAG_C Cuts at: 6170 6666 6170 Size: 496 6471 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3087 3351 3670 1163 Size: 573 1351 264 319 4460 Fragments arranged by size: 4460 1351 573 319 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3425 3539 5028 5113 6184 3425 Size: 114 1489 85 1071 4208 Fragments arranged by size: 4208 1489 1071 114 85 BsmI GAATG_Cn' Cuts at: 3096 3347 3760 4478 4571 3096 Size: 251 413 718 93 5492 Fragments arranged by size: 5492 718 413 251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3425 3539 4115 4286 5028 5113 6184 Size: 399 1635 114 576 171 742 85 1071 Cuts at: 6184 1391 Size: 2174 Fragments arranged by size: 2174 1635 1071 742 576 399 171 114 85 BspEI T'CCGG_A Cuts at: 6630 6630 Size: 6967 BspHI T'CATG_A Cuts at: 3202 3571 4856 6586 3202 Size: 369 1285 1730 3583 Fragments arranged by size: 3583 1730 1285 369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4225 4322 844 Size: 3381 97 3489 Fragments arranged by size: 3489 3381 97 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3922 5462 5636 65 Size: 1183 2674 1540 174 1396 Fragments arranged by size: 2674 1540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3388 5611 5798 1700 Size: 110 1578 2223 187 2869 Fragments arranged by size: 2869 2223 1578 187 110 BsrGI T'GTAC_A Cuts at: 96 2513 96 Size: 2417 4550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 3522 5027 6039 3522 Size: 1505 1012 4450 Fragments arranged by size: 4450 1505 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6967 BstBI TT'CG_AA Cuts at: 5771 5771 Size: 6967 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstXI CCAn_nnnn'nTGG Cuts at: 3218 3218 Size: 6967 BstYI r'GATC_y Cuts at: 1972 2231 2336 2835 2965 3678 3742 4246 4297 Size: 259 105 499 130 713 64 504 51 Cuts at: 4297 4664 5049 5066 6518 6604 6616 6962 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 713 504 499 385 367 346 259 130 105 86 64 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2046 2483 3447 2046 Size: 437 964 5566 Fragments arranged by size: 5566 964 437 BtgI C'CryG_G Cuts at: 513 1065 3937 513 Size: 552 2872 3543 Fragments arranged by size: 3543 2872 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6228 Fragments arranged by size: 6228 683 56 BtsI GCAGTG_nn' Cuts at: 4483 5277 5297 4483 Size: 794 20 6153 Fragments arranged by size: 6153 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6932 DraI TTT'AAA Cuts at: 4271 4271 Size: 6967 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5974 809 Size: 507 4658 1802 Fragments arranged by size: 4658 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3427 3934 4414 Size: 54 1425 50 275 18 1597 507 480 Cuts at: 4414 5303 6932 8 Size: 889 1629 43 Fragments arranged by size: 1629 1597 1425 889 507 480 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3240 4897 1353 Size: 1887 1657 3423 Fragments arranged by size: 3423 1887 1657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5554 5928 6074 387 Size: 1064 138 3965 374 146 1280 Fragments arranged by size: 3965 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2386 2583 3048 3332 3711 3929 5030 Size: 393 754 197 465 284 379 218 1101 Cuts at: 5030 5627 6414 6682 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1101 787 754 597 465 393 379 284 268 218 197 EcoRI G'AATT_C Cuts at: 4276 4654 4276 Size: 378 6589 EcoRV GAT'ATC Cuts at: 3577 3577 Size: 6967 FspI TGC'GCA Cuts at: 5473 5473 Size: 6967 HaeII r_GCGC'y Cuts at: 1678 6114 1678 Size: 4436 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2302 2334 2543 2575 3521 Size: 32 135 32 730 32 209 32 946 Cuts at: 3521 3553 5608 5640 5682 5714 1373 Size: 32 2055 32 42 32 2626 Fragments arranged by size: 2626 2055 946 730 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2084 2704 4311 4557 669 Size: 988 281 146 620 1607 246 3079 Fragments arranged by size: 3079 1607 988 620 281 246 146 HindIII A'AGCT_T Cuts at: 748 2105 748 Size: 1357 5610 HpaI GTT'AAC Cuts at: 4557 4557 Size: 6967 KpnI G_GTAC'C Cuts at: 4292 4292 Size: 6967 MfeI C'AATT_G Cuts at: 3898 4566 3898 Size: 668 6299 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3213 3542 6081 6265 1305 Size: 1908 329 2539 184 2007 Fragments arranged by size: 2539 2007 1908 329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3429 3936 10 Size: 54 1425 343 1597 507 3041 Fragments arranged by size: 3041 1597 1425 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2148 2352 2732 3301 3303 4925 Size: 648 976 6 204 380 569 2 1622 Cuts at: 4925 5284 5443 518 Size: 359 159 2042 Fragments arranged by size: 2042 1622 976 648 569 380 359 204 159 6 2 MspA1I CmG'CkG Cuts at: 1606 2363 4383 5060 6208 6453 1606 Size: 757 2020 677 1148 245 2120 Fragments arranged by size: 2120 2020 1148 757 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6415 NdeI CA'TA_TG Cuts at: 387 3380 387 Size: 2993 3974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6967 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NsiI A_TGCA'T Cuts at: 3173 3173 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 2903 3171 3175 3306 4001 4325 5870 Size: 97 1674 268 4 131 695 324 1545 Cuts at: 5870 1132 Size: 2229 Fragments arranged by size: 2229 1674 1545 695 324 268 131 97 4 PasI CC'CwG_GG Cuts at: 1348 4129 1348 Size: 2781 4186 PciI A'CATG_T Cuts at: 2899 3302 3997 5866 2899 Size: 403 695 1869 4000 Fragments arranged by size: 4000 1869 695 403 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6967 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6967 PsiI TTA'TAA Cuts at: 2715 3159 4537 2715 Size: 444 1378 5145 Fragments arranged by size: 5145 1378 444 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4133 4165 4133 Size: 32 6935 PstI C_TGCA'G Cuts at: 830 4319 830 Size: 3489 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 PvuII CAG'CTG Cuts at: 2363 2363 Size: 6967 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 3146 5215 6927 3146 Size: 2069 1712 3186 Fragments arranged by size: 3186 2069 1712 SfcI C'TryA_G Cuts at: 826 1045 3798 3975 4119 4315 5450 6131 6322 Size: 219 2753 177 144 196 1135 681 191 Cuts at: 6322 826 Size: 1471 Fragments arranged by size: 2753 1471 1135 681 219 196 191 177 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 2014 4294 1872 Size: 142 2280 4545 Fragments arranged by size: 4545 2280 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 3737 5071 5972 6234 820 Size: 197 926 1794 1334 901 262 1553 Fragments arranged by size: 1794 1553 1334 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4758 493 Size: 4265 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6967 SphI G_CATG'C Cuts at: 1132 3175 4325 1132 Size: 2043 1150 3774 Fragments arranged by size: 3774 2043 1150 SspI AAT'ATT Cuts at: 5 52 4891 5 Size: 47 4839 2081 Fragments arranged by size: 4839 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6967 StyI C'CwwG_G Cuts at: 513 1065 2068 2245 2332 3025 3712 4263 4406 Size: 552 1003 177 87 693 687 551 143 Cuts at: 4406 513 Size: 3074 Fragments arranged by size: 3074 1003 693 687 552 551 177 143 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 4994 5011 5164 5349 5794 325 Size: 1255 576 2838 17 153 185 445 1498 Fragments arranged by size: 2838 1498 1255 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2344 2513 3144 Size: 274 80 33 51 1443 367 169 631 Cuts at: 3144 3275 3307 5213 6925 96 Size: 131 32 1906 1712 138 Fragments arranged by size: 1906 1712 1443 631 367 274 169 138 131 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2287 2319 3060 3092 6927 6959 2287 Size: 32 741 32 3835 32 2295 Fragments arranged by size: 3835 2295 741 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 4303 4303 Size: 6967 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 2012 4292 1870 Size: 142 2280 4545 Fragments arranged by size: 4545 2280 142 XmnI GAAnn'nnTTC Cuts at: 5096 5096 Size: 6967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4775 276 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI