Restriction map for
pFN21AB6585
(Circular) (Six-base) MAPSORT of: pFN21AB6585.seq Check: 209 from: 1 to: 5836
pFN21AB6585 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2122 3646 278
Size: 53 83 186 936 586 1524 2468
Fragments arranged by size:
2468 1524 936 586 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 2770 3963 4336 1563
Size: 1207 1193 373 3063
Fragments arranged by size:
3063 1207 1193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2325 3899 5283 5551 1632
Size: 599 94 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1384 599 268 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 1870
Size: 73 1218 4545
Fragments arranged by size:
4545 1218 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2332 2915 3157 4612
Size: 325 217 627 481 64 583 242 1455
Cuts at: 4612 618
Size: 1842
Fragments arranged by size:
1842 1455 627 583 481 325 242 217
64
BanII G_rGCy'C
Cuts at: 1391 2058 3155 1391
Size: 667 1097 4072
Fragments arranged by size:
4072 1097 667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3115 3149 4027 4061 1903
Size: 34 1178 34 878 34 3678
Fragments arranged by size:
3678 1178 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2751 1548
Size: 1203 4633
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2743 4447 136
Size: 107 122 71 631 605 1071 1704 1525
Fragments arranged by size:
1704 1525 1071 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2262 2560 5831 2262
Size: 298 3271 2267
Fragments arranged by size:
3271 2267 298
BlpI GC'TnA_GC
Cuts at: 2622 3253 2622
Size: 631 5205
Bme1580I G_kGCm'C
Cuts at: 1790 2918 3897 5053 1790
Size: 1128 979 1156 2573
Fragments arranged by size:
2573 1156 1128 979
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3567 4525
Size: 416 53 83 399 24 837 1313 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1313 958 837 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2496 4496 1239
Size: 1257 2000 2579
Fragments arranged by size:
2579 2000 1257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2738 3961 4826 5124 2738
Size: 1223 865 298 3450
Fragments arranged by size:
3450 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4499 882
Size: 617 1281 1719 2219
Fragments arranged by size:
2219 1719 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 2170 3627 493
Size: 711 966 1457 2702
Fragments arranged by size:
2702 1457 966 711
BsaBI GATnn'nnATC
Cuts at: 2756 2993 3077 5830 2756
Size: 237 84 2753 2762
Fragments arranged by size:
2762 2753 237 84
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2119 3643 4025 4660
Size: 53 83 186 936 586 1524 382 635
Cuts at: 4660 275
Size: 1451
Fragments arranged by size:
1524 1451 936 635 586 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3007 4269 4667 4941 5088 5499 3007
Size: 1262 398 274 147 411 3344
Fragments arranged by size:
3344 1262 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 2642 2672 1178
Size: 30 165 30 1066 30 143 30 4342
Fragments arranged by size:
4342 1066 165 143 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 2075 2398 2975 5039 5535 2075
Size: 323 577 2064 496 2376
Fragments arranged by size:
2376 2064 577 496 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3286 4047 5075 1540
Size: 275 186 943 342 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 2952 3347 3440 2952
Size: 395 93 5348
Fragments arranged by size:
5348 395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2918 3155 3897 3982 5053 1391
Size: 399 268 860 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 742 399 268 237 85
BspEI T'CCGG_A
Cuts at: 3007 5499 3007
Size: 2492 3344
BspHI T'CATG_A
Cuts at: 2577 2856 3725 5455 2577
Size: 279 869 1730 2958
Fragments arranged by size:
2958 1730 869 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2934 2997 4331 4505 65
Size: 1183 1686 63 1334 174 1396
Fragments arranged by size:
1686 1396 1334 1183 174 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 4480 4667 1700
Size: 110 256 2414 187 2869
Fragments arranged by size:
2869 2414 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2262 2545 2560 2583 3166 3533 3918 3935
Size: 290 283 15 23 583 367 385 17
Cuts at: 3935 5387 5473 5485 5831 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 290 283
86 23 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 2694 3352 4146 4166 2025
Size: 669 658 794 20 3695
Fragments arranged by size:
3695 794 669 658 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2554 2691 3283
Size: 54 1425 50 275 18 724 137 592
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 724 592 275 137 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2301 2717 3766 1353
Size: 657 291 416 1049 3423
Fragments arranged by size:
3423 1049 657 416 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2325 2496 3899 4496 5283 5551
Size: 393 599 94 171 1403 597 787 268
Cuts at: 5551 1239
Size: 1524
Fragments arranged by size:
1524 1403 787 599 597 393 268 171
94
EcoO109I rG'GnC_Cy
Cuts at: 2884 2884
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2469 2501 4477
Size: 32 135 32 461 32 404 32 1976
Cuts at: 4477 4509 4551 4583 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1976 461 404 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2693 10
Size: 54 1425 343 861 3153
Fragments arranged by size:
3153 1425 861 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2813 2921 3794 4153 4312 518
Size: 648 1242 405 108 873 359 159 2042
Fragments arranged by size:
2042 1242 873 648 405 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2778 3252 3929 5077 5322 1606
Size: 1172 474 677 1148 245 2120
Fragments arranged by size:
2120 1172 1148 677 474 245
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2091 1348
Size: 743 5093
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2637 2637
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 3905 3937 5418 5450 2777
Size: 32 1096 32 1481 32 3163
Fragments arranged by size:
3163 1481 1096 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2301 2301
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 3184 4319 5000 5191 826
Size: 219 2139 1135 681 191 1471
Fragments arranged by size:
2139 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3940 4841 5103 820
Size: 197 926 774 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 774 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2031 2346 3275 513
Size: 552 966 315 929 3074
Fragments arranged by size:
3074 966 929 552 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2504 2958 2989 3863 3880 4033
Size: 1255 582 342 454 31 874 17 153
Cuts at: 4033 4218 4663 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 874 582 454 445 342 185
153 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 5796 5828 2642
Size: 32 3122 32 2650
Fragments arranged by size:
3122 2650 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2120 3644 276
Size: 53 83 186 936 586 1524 2468
Fragments arranged by size:
2468 1524 936 586 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI