(Circular) (Six-base) MAPSORT of: pFN21AB6579.seq Check: 4716 from: 1 to: 5836 pFN21AB6579 5836 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 3899 5283 5551 1632 Size: 599 1668 1384 268 1917 Fragments arranged by size: 1917 1668 1384 599 268 AfeI AGC'GCT Cuts at: 2771 2771 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 3579 5151 3579 Size: 1572 4264 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2303 3161 1870 Size: 73 360 858 4545 Fragments arranged by size: 4545 858 360 73 AvrII C'CTAG_G Cuts at: 2090 2090 Size: 5836 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2268 2915 2971 3157 4612 Size: 325 217 627 481 647 56 186 1455 Cuts at: 4612 618 Size: 1842 Fragments arranged by size: 1842 1455 647 627 481 325 217 186 56 BanII G_rGCy'C Cuts at: 1391 3155 1391 Size: 1764 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 928 Size: 971 123 4742 Fragments arranged by size: 4742 971 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3115 3149 4027 4061 1903 Size: 34 1178 34 878 34 3678 Fragments arranged by size: 3678 1178 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2210 3728 4938 2210 Size: 1518 1210 3108 Fragments arranged by size: 3108 1518 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2420 2743 Size: 107 122 71 631 605 421 327 323 Cuts at: 2743 4447 136 Size: 1704 1525 Fragments arranged by size: 1704 1525 631 605 421 327 323 122 107 71 BglII A'GATC_T Cuts at: 2262 5831 2262 Size: 3569 2267 BlpI GC'TnA_GC Cuts at: 2622 3253 2622 Size: 631 5205 Bme1580I G_kGCm'C Cuts at: 1790 2400 2502 2918 3897 5053 1790 Size: 610 102 416 979 1156 2573 Fragments arranged by size: 2573 1156 979 610 416 102 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3567 4525 Size: 416 53 83 399 24 837 1313 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1313 958 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4496 1239 Size: 3257 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2378 2831 4499 882 Size: 617 516 363 453 1668 2219 Fragments arranged by size: 2219 1668 617 516 453 363 BsaAI yAC'GTr Cuts at: 493 1204 2170 2410 2665 3627 493 Size: 711 966 240 255 962 2702 Fragments arranged by size: 2702 966 962 711 255 240 BsaBI GATnn'nnATC Cuts at: 2582 2993 3077 5830 2582 Size: 411 84 2753 2588 Fragments arranged by size: 2753 2588 411 84 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2136 2484 3643 Size: 53 83 186 936 552 51 348 1159 Cuts at: 3643 4025 4660 275 Size: 382 635 1451 Fragments arranged by size: 1451 1159 936 635 552 382 348 186 83 53 51 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 2642 2672 1178 Size: 30 165 30 630 30 579 30 4342 Fragments arranged by size: 4342 630 579 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2321 2695 2698 2701 1186 Size: 1135 374 3 3 4321 Fragments arranged by size: 4321 1135 374 3 3 BseYI C'CCAG_C Cuts at: 2975 5039 5535 2975 Size: 2064 496 3276 Fragments arranged by size: 3276 2064 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2700 3286 4047 5075 1540 Size: 275 186 699 586 761 1028 2301 Fragments arranged by size: 2301 1028 761 699 586 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2313 3897 3982 5053 2229 Size: 84 1584 85 1071 3012 Fragments arranged by size: 3012 1584 1071 85 84 BsmI GAATG_Cn' Cuts at: 2829 3347 3440 2829 Size: 518 93 5225 Fragments arranged by size: 5225 518 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2780 2780 Size: 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2313 2400 2502 2918 3155 3897 Size: 399 439 84 87 102 416 237 742 Cuts at: 3897 3982 5053 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 416 399 237 102 87 85 84 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 2856 3725 5455 2856 Size: 869 1730 3237 Fragments arranged by size: 3237 1730 869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2737 3191 844 Size: 1893 454 3489 Fragments arranged by size: 3489 1893 454 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2456 3104 4480 4667 1700 Size: 110 256 390 648 1376 187 2869 Fragments arranged by size: 2869 1376 648 390 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2033 2834 3896 4908 2033 Size: 801 1062 1012 2961 Fragments arranged by size: 2961 1062 1012 801 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2262 2583 3166 3533 3918 3935 5387 5473 Size: 290 321 583 367 385 17 1452 86 Cuts at: 5473 5485 5831 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 321 290 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5284 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2983 487 Size: 683 56 1757 3340 Fragments arranged by size: 3340 1757 683 56 BtsI GCAGTG_nn' Cuts at: 3352 4146 4166 3352 Size: 794 20 5022 Fragments arranged by size: 5022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DraIII CAC_nnn'GTG Cuts at: 2497 2497 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2747 4843 809 Size: 507 1431 2096 1802 Fragments arranged by size: 2096 1802 1431 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 2697 3283 Size: 54 1425 50 275 18 582 285 586 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 586 582 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2697 3283 1537 Size: 275 885 586 4090 Fragments arranged by size: 4090 885 586 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2798 3766 1353 Size: 1445 968 3423 Fragments arranged by size: 3423 1445 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 3899 4496 5283 5551 1239 Size: 393 599 1668 597 787 268 1524 Fragments arranged by size: 1668 1524 787 599 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2446 2446 Size: 5836 EcoO109I rG'GnC_Cy Cuts at: 2332 2884 2332 Size: 552 5284 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FseI GG_CCGG'CC Cuts at: 3108 3108 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2773 4983 1678 Size: 1095 2210 2531 Fragments arranged by size: 2531 2210 1095 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 4477 4509 4551 Size: 32 135 32 461 32 2412 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 2412 461 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 10 Size: 54 1425 343 582 3432 Fragments arranged by size: 3432 1425 582 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2813 2921 3794 4153 4312 518 Size: 648 1242 405 108 873 359 159 2042 Fragments arranged by size: 2042 1242 873 648 405 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2621 2778 3252 3929 5077 5322 1606 Size: 1015 157 474 677 1148 245 2120 Fragments arranged by size: 2120 1148 1015 677 474 245 157 NaeI GCC'GGC Cuts at: 2068 3106 2068 Size: 1038 4798 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NgoMIV G'CCGG_C Cuts at: 2066 3104 2066 Size: 1038 4798 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 2950 3194 4739 1132 Size: 97 1721 244 1545 2229 Fragments arranged by size: 2229 1721 1545 244 97 PasI CC'CwG_GG Cuts at: 1348 2930 1348 Size: 1582 4254 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2884 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2621 2621 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2798 2798 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2420 2420 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 2950 3194 1132 Size: 1818 244 3774 Fragments arranged by size: 3774 1818 244 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2090 2346 3275 513 Size: 552 1025 256 929 3074 Fragments arranged by size: 3074 1025 929 552 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 2989 3863 3880 4033 4218 4663 Size: 1255 924 485 874 17 153 185 445 Cuts at: 4663 325 Size: 1498 Fragments arranged by size: 1498 1255 924 874 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 5796 5828 2642 Size: 32 3122 32 2650 Fragments arranged by size: 3122 2650 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2483 1316 Size: 1167 4669 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2539 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI