Restriction map for
pFN21AE0174
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AccIII AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbsI BcgI BcnI
BfrI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HapII HgaI HindIII
HpaII Ksp632I KspI MamI MluI
MluNI MroI MscI MspI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 156 GACGTC GACGT^C
Acc65I 36 GGTACC G^GTACC
AccI 146 GTAGAC GT^[AC][GT]AC
AccII 11 CGCG CG^CG
AclI 646 AACGTT AA^CGTT
AlwI 448 GGATC GGATC(4/5)
Asp718I 36 GGTACC G^GTACC
AvaII 76 GGTCC G^G[AT]CC
BbeI 123 GGCGCC GGCGC^C
BciVI 110 GGATAC GTATCC(6/5)
BclI 831 TGATCA T^GATCA
BglI 126 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 289 GAGGAG GAGGAG(10/8)
BsiEI 783 CGATCG CG[GA][CT]^CG
BspMI 774 GCAGGT ACCTGC(4/8)
BsrI 378 ACTGG ACTGG(1/-1)
BssHII 10 GCGCGC G^CGCGC
BstUI 11 CGCG CG^CG
BstYI 448 GGATCT [GA]^GATC[CT]
EcoNI 317 CCTGCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 120 GCGGG CCCGC(4/6)
FbaI 831 TGATCA T^GATCA
HaeII 123 GGCGCC [GA]GCGC^[CT]
HincII 913 GTTAAC GT[CT]^[GA]AC
HindII 913 GTTAAC GT[CT]^[GA]AC
HinfI 438 GATTC G^A[ACGT]TC
HpaI 913 GTTAAC GTT^AAC
HphI 834 TCACC GGTGA(8/7)
KasI 123 GGCGCC G^GCGCC
KpnI 36 GGTACC GGTAC^C
MfeI 485 CAATTG C^AATTG
MflI 448 GGATCT [GA]^GATC[CT]
MslI 798 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 485 CAATTG C^AATTG
MvnI 11 CGCG CG^CG
NarI 123 GGCGCC GG^CGCC
NcoI 732 CCATGG C^CATGG
NspI 611 GCATGC [GA]CATG^[CT]
Psp1406I 646 AACGTT AA^CGTT
PstI 533 CTGCAG CTGCA^G
PvuI 783 CGATCG CGAT^CG
ScaI 789 AGTACT AGT^ACT
SfoI 123 GGCGCC GGC^GCC
SphI 611 GCATGC GCATG^C
TfiI 438 GATTC G^A[AT]TC
XhoII 448 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 123 GGCGCC G[GA]^CG[CT]C
156 GACGTC
BanI 36 GGTACC G^G[CT][GA]CC
123 GGCGCC
BbvCI 90 CCTCAGC CCTCAGC(-5/-2)
115 CCTCAGC
BfaI 307 CTAG C^TAG
452 CTAG
BsaHI 123 GGCGCC G[GA]^CG[CT]C
156 GACGTC
BsmFI 67 GTCCC GGGAC(10/14)
77 GTCCC
Cfr13I 76 GGTCC G^G[ACGT]CC
430 GGGCC
Hin1I 123 GGCGCC G[GA]^CG[CT]C
156 GACGTC
MaeI 307 CTAG C^TAG
452 CTAG
MaeII 157 ACGT A^CGT
647 ACGT
MboII 631 GAAGA GAAGA(8/7)
236 TCTTC
PvuII 20 CAGCTG CAG^CTG
530 CAGCTG
Sau96I 76 GGTCC G^G[ACGT]CC
430 GGGCC
TaqI 177 TCGA T^CGA
786 TCGA
TthHB8I 177 TCGA T^CGA
786 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I CfoI DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I ItaI MaeIII MboI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI