Restriction map for
pFN21AE0174
(Circular) (Six-base) MAPSORT of: pFN21AE0174.seq Check: 6157 from: 1 to: 5623
pFN21AE0174 5623 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2165 3433 278
Size: 53 83 186 936 629 1268 2468
Fragments arranged by size:
2468 1268 936 629 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2152 2966 1937
Size: 215 814 4594
Fragments arranged by size:
4594 814 215
Acc65I G'GTAC_C
Cuts at: 2041 2944 2041
Size: 903 4720
AclI AA'CG_TT
Cuts at: 1563 2652 2922 3750 4123 1563
Size: 1089 270 828 373 3063
Fragments arranged by size:
3063 1089 828 373 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 3686 5070 5338 1632
Size: 1023 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 2807 1166
Size: 1641 3982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AlwNI CAG_nnn'CTG
Cuts at: 3366 4938 3366
Size: 1572 4051
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2932 3158 3310 5419 1493
Size: 1439 226 152 2109 1697
Fragments arranged by size:
2109 1697 1439 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2948 1870
Size: 73 1005 4545
Fragments arranged by size:
4545 1005 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2535 2568 2535
Size: 33 5590
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2128 2944 4399 618
Size: 325 217 627 254 87 816 1455 1842
Fragments arranged by size:
1842 1455 816 627 325 254 217 87
BanII G_rGCy'C
Cuts at: 1391 2942 1391
Size: 1551 4072
BbeI G_GCGC'C
Cuts at: 2132 2132
Size: 5623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4652
BbvCI CC'TCA_GC
Cuts at: 2096 2121 2096
Size: 25 5598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2109 3515 4725 2109
Size: 1406 1210 3007
Fragments arranged by size:
3007 1406 1210
BclI T'GATC_A
Cuts at: 1548 2836 1548
Size: 1288 4335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2137 4234 136
Size: 107 122 71 631 605 465 2097 1525
Fragments arranged by size:
2097 1525 631 605 465 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 3040 3040
Size: 5623
Bme1580I G_kGCm'C
Cuts at: 1790 3684 4840 1790
Size: 1894 1156 2573
Fragments arranged by size:
2573 1894 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 3354 4312
Size: 416 53 83 399 24 976 961 958
Cuts at: 4312 442
Size: 1753
Fragments arranged by size:
1753 976 961 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4283 1239
Size: 3044 2579
Bpu10I CC'TnA_GC
Cuts at: 2096 2121 2096
Size: 25 5598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4286 882
Size: 617 2787 2219
Fragments arranged by size:
2787 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 2162 3430 3812
Size: 53 83 186 936 596 33 1268 382
Cuts at: 3812 4447 275
Size: 635 1451
Fragments arranged by size:
1451 1268 936 635 596 382 186 83
53 33
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2124 2154 1178
Size: 30 165 30 721 30 4647
Fragments arranged by size:
4647 721 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4500
BseYI C'CCAG_C
Cuts at: 2055 2061 2143 4826 5322 2055
Size: 6 82 2683 496 2356
Fragments arranged by size:
2683 2356 496 82 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5050
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3073 3834 4862 1540
Size: 275 186 790 282 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 3684 3769 4840 3684
Size: 85 1071 4467
Fragments arranged by size:
4467 1071 85
BsmI GAATG_Cn'
Cuts at: 3134 3227 3134
Size: 93 5530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2942 3684 3769 4840 1391
Size: 399 1152 742 85 1071 2174
Fragments arranged by size:
2174 1152 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 2978 844
Size: 1926 208 3489
Fragments arranged by size:
3489 1926 208
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 1394
Size: 429 1687 3507
Fragments arranged by size:
3507 1687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4267 4454 1700
Size: 110 2457 187 2869
Fragments arranged by size:
2869 2457 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5623
BssHII G'CGCG_C
Cuts at: 1925 2015 1925
Size: 90 5533
BssSI C'ACGA_G
Cuts at: 3683 4695 3683
Size: 1012 4611
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstYI r'GATC_y
Cuts at: 1972 2453 2953 3320 3705 3722 5174 5260 5272
Size: 481 500 367 385 17 1452 86 12
Cuts at: 5272 5618 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 500 481 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2089 2132 2737 513
Size: 552 939 85 43 605 3399
Fragments arranged by size:
3399 939 605 552 85 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 2686 3139 3933 3953 2686
Size: 453 794 20 4356
Fragments arranged by size:
4356 794 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 3959 5588
Size: 54 1425 50 275 18 1240 889 1629
Cuts at: 5588 8
Size: 43
Fragments arranged by size:
1629 1425 1240 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 1353
Size: 2200 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2655 3686 4283 5070 5338 1239
Size: 393 1023 1031 597 787 268 1524
Fragments arranged by size:
1524 1031 1023 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2326 2326
Size: 5623
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 5623
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2132 4770 1678
Size: 454 2638 2531
Fragments arranged by size:
2638 2531 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2891 2923 4264
Size: 32 135 32 779 32 508 32 1341
Cuts at: 4264 4296 4338 4370 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 779 508 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 2967 3213 669
Size: 988 281 982 47 246 3079
Fragments arranged by size:
3079 988 982 281 246 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5623
HpaI GTT'AAC
Cuts at: 2920 3213 2920
Size: 293 5330
KasI G'GCGC_C
Cuts at: 2128 2128
Size: 5623
KpnI G_GTAC'C
Cuts at: 2045 2948 2045
Size: 903 4720
MfeI C'AATT_G
Cuts at: 2490 3222 2490
Size: 732 4891
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2030 2329 2431 4737 4921 1305
Size: 725 299 102 2306 184 2007
Fragments arranged by size:
2306 2007 725 299 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3801
Fragments arranged by size:
3801 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 3581 3940 4099 518
Size: 648 1641 774 359 159 2042
Fragments arranged by size:
2042 1641 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2125 2537 3039 3716 4864 5109 1606
Size: 421 98 412 502 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 502 421 412 245 98
NarI GG'CG_CC
Cuts at: 2129 2129
Size: 5623
NcoI C'CATG_G
Cuts at: 513 1065 2004 2737 513
Size: 552 939 733 3399
Fragments arranged by size:
3399 939 733 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2620 2981 4526 1132
Size: 97 1391 361 1545 2229
Fragments arranged by size:
2229 1545 1391 361 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5623
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5623
PfoI T'CCnGG_A
Cuts at: 2230 2230
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 2208 2649 3193 2208
Size: 441 544 4638
Fragments arranged by size:
4638 544 441
PstI C_TGCA'G
Cuts at: 830 2542 2975 830
Size: 1712 433 3478
Fragments arranged by size:
3478 1712 433
PvuI CG_AT'CG
Cuts at: 2001 2791 2001
Size: 790 4833
PvuII CAG'CTG
Cuts at: 2027 2537 2027
Size: 510 5113
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 2796 3871 5583 2796
Size: 1075 1712 2836
Fragments arranged by size:
2836 1712 1075
SfcI C'TryA_G
Cuts at: 826 1045 2532 2538 2913 2971 4106 4787 4978
Size: 219 1487 6 375 58 1135 681 191
Cuts at: 4978 826
Size: 1471
Fragments arranged by size:
1487 1471 1135 681 375 219 191 58
6
SfoI GGC'GCC
Cuts at: 2130 2130
Size: 5623
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5623
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3727 4628 4890 820
Size: 197 926 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2620 2981 1132
Size: 1488 361 3774
Fragments arranged by size:
3774 1488 361
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2461 2737 2762 3062 513
Size: 552 939 457 276 25 300 3074
Fragments arranged by size:
3074 939 552 457 300 276 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 3650 3667 3820 4005 4450 325
Size: 1255 489 1581 17 153 185 445 1498
Fragments arranged by size:
1581 1498 1255 489 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 2794 3869
Size: 274 80 33 51 1443 681 136 1075
Cuts at: 3869 5581 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1075 681 274 138 136 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2124 2156 2157 2189 5583 5615 2124
Size: 32 1 32 3394 32 2132
Fragments arranged by size:
3394 2132 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 3752 3752
Size: 5623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2163 3431 276
Size: 53 83 186 936 629 1268 2468
Fragments arranged by size:
2468 1268 936 629 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI