Restriction map for
pFN21AE0174
(Linear) (Six-base) MAPSORT of: KIEE0174.seq Check: 4040 from: 1 to: 918
KIEE0174.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 0 160 918
Size: 160 758
AccI GT'mk_AC
Cuts at: 0 147 918
Size: 147 771
Acc65I G'GTAC_C
Cuts at: 0 36 918
Size: 36 882
AclI AA'CG_TT
Cuts at: 0 647 918
Size: 647 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 918
Size: 650 268
AleI CACnn'nnGTG
Cuts at: 0 802 918
Size: 802 116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 530 563 918
Size: 530 33 355
Fragments arranged by size:
530 355 33
BanI G'GyrC_C
Cuts at: 0 36 123 918
Size: 36 87 795
Fragments arranged by size:
795 87 36
BbeI G_GCGC'C
Cuts at: 0 127 918
Size: 127 791
BbvCI CC'TCA_GC
Cuts at: 0 91 116 918
Size: 91 25 802
Fragments arranged by size:
802 91 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 104 918
Size: 104 814
BclI T'GATC_A
Cuts at: 0 831 918
Size: 831 87
BglI GCCn_nnn'nGGC
Cuts at: 0 132 918
Size: 132 786
BmrI ACTGGGnnnn_n'
Cuts at: 0 388 918
Size: 388 530
Bpu10I CC'TnA_GC
Cuts at: 0 91 116 918
Size: 91 25 802
Fragments arranged by size:
802 91 25
BsaHI Gr'CG_yC
Cuts at: 0 124 157 918
Size: 124 33 761
Fragments arranged by size:
761 124 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 119 149 918
Size: 119 30 769
Fragments arranged by size:
769 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 918
Size: 304 614
BseYI C'CCAG_C
Cuts at: 0 50 56 138 918
Size: 50 6 82 780
Fragments arranged by size:
780 82 50 6
BsiEI CG_ry'CG
Cuts at: 0 786 918
Size: 786 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 918
Size: 765 153
BssHII G'CGCG_C
Cuts at: 0 10 918
Size: 10 908
BstYI r'GATC_y
Cuts at: 0 448 918
Size: 448 470
BtgI C'CryG_G
Cuts at: 0 84 127 732 918
Size: 84 43 605 186
Fragments arranged by size:
605 186 84 43
BtsI GCAGTG_nn'
Cuts at: 0 681 918
Size: 681 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 918
Size: 650 268
EcoNI CCTnn'n_nnAGG
Cuts at: 0 321 918
Size: 321 597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 418 918
Size: 418 500
HaeII r_GCGC'y
Cuts at: 0 127 918
Size: 127 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 886 918
Size: 346 32 508 32
Fragments arranged by size:
508 346 32 32
HincII GTy'rAC
Cuts at: 0 915 918
Size: 915 3
HpaI GTT'AAC
Cuts at: 0 915 918
Size: 915 3
KasI G'GCGC_C
Cuts at: 0 123 918
Size: 123 795
KpnI G_GTAC'C
Cuts at: 0 40 918
Size: 40 878
MfeI C'AATT_G
Cuts at: 0 485 918
Size: 485 433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 324 426 918
Size: 25 299 102 492
Fragments arranged by size:
492 299 102 25
MslI CAynn'nnrTG
Cuts at: 0 802 918
Size: 802 116
MspA1I CmG'CkG
Cuts at: 0 22 120 532 918
Size: 22 98 412 386
Fragments arranged by size:
412 386 98 22
NarI GG'CG_CC
Cuts at: 0 124 918
Size: 124 794
NcoI C'CATG_G
Cuts at: 0 732 918
Size: 732 186
NspI r_CATG'y
Cuts at: 0 615 918
Size: 615 303
PfoI T'CCnGG_A
Cuts at: 0 225 918
Size: 225 693
PsiI TTA'TAA
Cuts at: 0 203 644 918
Size: 203 441 274
Fragments arranged by size:
441 274 203
PstI C_TGCA'G
Cuts at: 0 537 918
Size: 537 381
PvuI CG_AT'CG
Cuts at: 0 786 918
Size: 786 132
PvuII CAG'CTG
Cuts at: 0 22 532 918
Size: 22 510 386
Fragments arranged by size:
510 386 22
ScaI AGT'ACT
Cuts at: 0 791 918
Size: 791 127
SfcI C'TryA_G
Cuts at: 0 527 533 908 918
Size: 527 6 375 10
Fragments arranged by size:
527 375 10 6
SfoI GGC'GCC
Cuts at: 0 125 918
Size: 125 793
SphI G_CATG'C
Cuts at: 0 615 918
Size: 615 303
StyI C'CwwG_G
Cuts at: 0 456 732 757 918
Size: 456 276 25 161
Fragments arranged by size:
456 276 161 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 918
Size: 64 854
TatI w'GTAC_w
Cuts at: 0 653 789 918
Size: 653 136 129
Fragments arranged by size:
653 136 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 152 184 918
Size: 119 32 1 32 734
Fragments arranged by size:
734 119 32 32 1
ZraI GAC'GTC
Cuts at: 0 158 918
Size: 158 760
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AleI BaeI BanI
BbeI BbvCI BciVI BclI BglI BmrI Bpu10I BsaHI
BsaXI BseRI BseYI BsiEI BspMI BssHII BstYI BtgI
BtsI Eco57MI EcoNI FalI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MmeI MslI MspA1I NarI NcoI
NspI PfoI PsiI PstI PvuI PvuII ScaI SfcI
SfoI SphI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI AfeI AflII AflIII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbsI BcgI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII MluI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI