Restriction map for
pFN21AA1844
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbvCI BcgI BcnI BglI BlnI
BsaBI BsaI BsaJI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FauI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1759 CGCG CG^CG
AcyI 142 GACGCC G[GA]^CG[CT]C
AflII 82 CTTAAG C^TTAAG
AlwNI 1172 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1547 AGCGCT AGC^GCT
Asp700I 1926 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 314 GTGCTC G[AT]GC[AT]^C
BbrPI 1653 CACGTG CAC^GTG
BciVI 1874 GGATAC GTATCC(6/5)
BfrI 82 CTTAAG C^TTAAG
BlpI 1270 GCTCAGC GC^T[ACGT]AGC
BmyI 314 GTGCTC G[AGT]GC[ACT]^C
BpmI 1445 CTCCAG CTGGAG(16/14)
Bpu1102I 1270 GCTCAGC GC^T[ACGT]AGC
BsaAI 1653 CACGTG [CT]AC^GT[GA]
BsaHI 142 GACGCC G[GA]^CG[CT]C
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BsiEI 48 CGACCG CG[GA][CT]^CG
BsiHKAI 314 GTGCTC G[AT]GC[AT]^C
BsmI 621 GCATTC GAATGC(1/-1)
Bsp1286I 314 GTGCTC G[AGT]GC[ACT]^C
BspHI 1518 TCATGA T^CATGA
BspLU11I 1790 ACATGT A^CATGT
BstUI 1759 CGCG CG^CG
CelII 1270 GCTCAGC GC^T[ACGT]AGC
DraII 1073 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 1547 AGCGCT AGC^GCT
EcoO109I 1073 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1607 ATGCAT ATGCA^T
HaeII 1547 AGCGCT [GA]GCGC^[CT]
Hin1I 142 GACGCC G[GA]^CG[CT]C
HincII 532 GTCAAC GT[CT]^[GA]AC
HindII 532 GTCAAC GT[CT]^[GA]AC
MvnI 1759 CGCG CG^CG
NdeI 1346 CATATG CA^TATG
NsiI 1607 ATGCAT ATGCA^T
NspI 1790 ACATGT [GA]CATG^[CT]
PflMI 1570 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1653 CACGTG CAC^GTG
PmlI 1653 CACGTG CAC^GTG
PpuMI 1073 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1463 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1175 CAGCTG CAG^CTG
RcaI 1518 TCATGA T^CATGA
SexAI 1575 ACCTGGT A^CC[AT]GGT
SpeI 589 ACTAGT A^CTAGT
SspI 655 AATATT AAT^ATT
Van91I 1570 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 746 CCATCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1926 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 151 GATCC GGATC(4/5)
1649 GATCC
BglII 181 AGATCT A^GATCT
1825 AGATCT
BseRI 1412 CTCCTC GAGGAG(10/8)
1532 CTCCTC
BsmBI 1250 CGTCTC CGTCTC(1/5)
140 GAGACG
BssKI 75 CCAGG ^CC[ACGT]GG
1576 CCTGG
BstAPI 1609 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1726 GCAGATCATGC
BstNI 75 CCAGG CC^[AT]GG
1576 CCTGG
BstXI 478 CCAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
602 CCAGTGGAATGG
CfoI 1548 GCGC GCG^C
1768 GCGC
EcoRI 1635 GAATTC G^AATTC
1926 GAATTC
EcoRII 75 CCAGG ^CC[AT]GG
1576 CCTGG
HaeIII 231 GGCC GG^CC
1721 GGCC
HapII 51 CCGG C^CGG
146 CCGG
HgaI 142 GACGC GACGC(5/10)
1330 GCGTC
HhaI 1548 GCGC GCG^C
1768 GCGC
HinP1I 1548 GCGC G^CGC
1768 GCGC
HpaII 51 CCGG C^CGG
146 CCGG
MfeI 444 CAATTG C^AATTG
1093 CAATTG
MspA1I 1022 CAGCGG C[AC]G^C[GT]G
1175 CAGCTG
MspI 51 CCGG C^CGG
146 CCGG
MunI 444 CAATTG C^AATTG
1093 CAATTG
MvaI 75 CCAGG CC^[AT]GG
1576 CCTGG
SapI 316 GCTCTTC GCTCTTC(1/4)
419 GCTCTTC
ScrFI 75 CCAGG CC^[ACGT]GG
1576 CCTGG
TfiI 388 GATTC G^A[AT]TC
919 GAATC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AvaII BbsI BbvI BclI
BfaI BpuAI BsgI BsiYI BslI
BsmAI BsmFI BsrI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII EarI
FbaI Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScaI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII