Restriction map for
pFN21AA1844
(Circular) (Six-base) MAPSORT of: pFN21AA1844.seq Check: 7718 from: 1 to: 6646
pFN21AA1844 6646 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 3009 3059 3533 3676 4709 6093 6361
Size: 729 648 50 474 143 1033 1384 268
Cuts at: 6361 1632
Size: 1917
Fragments arranged by size:
1917 1384 1033 729 648 474 268 143
50
AfeI AGC'GCT
Cuts at: 3554 3554
Size: 6646
AflII C'TTAA_G
Cuts at: 820 1017 2087 820
Size: 197 1070 5379
Fragments arranged by size:
5379 1070 197
AflIII A'CryG_T
Cuts at: 1203 3659 3712 3795 5545 6522 1203
Size: 2456 53 83 1750 977 1327
Fragments arranged by size:
2456 1750 1327 977 83 53
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 3693 1166
Size: 2527 4119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AlwNI CAG_nnn'CTG
Cuts at: 3182 4389 5961 3182
Size: 1207 1572 3867
Fragments arranged by size:
3867 1572 1207
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 2302 2335 3640 3804 3823 3852 3931 3955
Size: 809 33 1305 164 19 29 79 24
Cuts at: 3955 4181 4333 6442 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1305 809 226 164 152 79
33 29 24 19
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 3971 1870
Size: 73 2028 4545
Fragments arranged by size:
4545 2028 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3615 3648 3615
Size: 33 6613
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3967 5422 618
Size: 325 217 627 2180 1455 1842
Fragments arranged by size:
2180 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3965 1391
Size: 2574 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2625 2970 3539 928
Size: 971 726 345 569 4035
Fragments arranged by size:
4035 971 726 569 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3873 4538 5748 3873
Size: 665 1210 4771
Fragments arranged by size:
4771 1210 665
BclI T'GATC_A
Cuts at: 1548 2122 2821 3408 1548
Size: 574 699 587 4786
Fragments arranged by size:
4786 699 587 574
BfrBI ATG'CAT
Cuts at: 3614 3614
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5257 136
Size: 107 122 71 631 605 3585 1525
Fragments arranged by size:
3585 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2186 3830 6641 2186
Size: 1644 2811 2191
Fragments arranged by size:
2811 2191 1644
BlpI GC'TnA_GC
Cuts at: 3276 4063 3276
Size: 787 5859
Bme1580I G_kGCm'C
Cuts at: 1790 4707 5863 1790
Size: 2917 1156 2573
Fragments arranged by size:
2917 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2976 1318
Size: 1658 4988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 4377 5335
Size: 416 53 83 399 24 689 2271 958
Cuts at: 5335 442
Size: 1753
Fragments arranged by size:
2271 1753 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3435 5306 1239
Size: 2196 1871 2579
Fragments arranged by size:
2579 2196 1871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3252 3541 4771 5636 5934 3252
Size: 289 1230 865 298 3964
Fragments arranged by size:
3964 1230 865 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5309 882
Size: 617 3810 2219
Fragments arranged by size:
3810 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3660 4437 493
Size: 711 2456 777 2702
Fragments arranged by size:
2702 2456 777 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 4453 4835 5470
Size: 53 83 186 936 615 2305 382 635
Cuts at: 5470 275
Size: 1451
Fragments arranged by size:
2305 1451 936 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2055 5079 5477 5751 5898 6309 2055
Size: 3024 398 274 147 411 2392
Fragments arranged by size:
3024 2392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6421
Fragments arranged by size:
6421 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3408 3528 1186
Size: 2222 120 4304
Fragments arranged by size:
4304 2222 120
BseYI C'CCAG_C
Cuts at: 3294 5849 6345 3294
Size: 2555 496 3595
Fragments arranged by size:
3595 2555 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2886 2991 3660 1163
Size: 573 1150 105 669 4149
Fragments arranged by size:
4149 1150 669 573 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 4096 4857 5885 1540
Size: 275 186 55 2040 761 1028 2301
Fragments arranged by size:
2301 2040 1028 761 275 186 55
BsiHKAI G_wGCw'C
Cuts at: 2323 4707 4792 5863 2323
Size: 2384 85 1071 3106
Fragments arranged by size:
3106 2384 1071 85
BsmI GAATG_Cn'
Cuts at: 2626 4157 4250 2626
Size: 1531 93 5022
Fragments arranged by size:
5022 1531 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2139 3261 2139
Size: 1122 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 3965 4707 4792 5863 1391
Size: 399 533 1642 742 85 1071 2174
Fragments arranged by size:
2174 1642 1071 742 533 399 85
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 3523 4535 6265 3523
Size: 1012 1730 3904
Fragments arranged by size:
3904 1730 1012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4001 844
Size: 3157 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4533 1394
Size: 429 2710 3507
Fragments arranged by size:
3507 2710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5141 5315 65
Size: 1183 3893 174 1396
Fragments arranged by size:
3893 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5290 5477 1700
Size: 110 3480 187 2869
Fragments arranged by size:
3480 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3620 3737 794
Size: 2826 117 3703
Fragments arranged by size:
3703 2826 117
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 2490 2614 2490
Size: 124 6522
BstYI r'GATC_y
Cuts at: 1972 2155 2186 3830 3976 4343 4728 4745 6197
Size: 183 31 1644 146 367 385 17 1452
Cuts at: 6197 6283 6295 6641 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1644 1452 385 367 346 183 146
86 31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5155
Fragments arranged by size:
5155 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 3197 4162 4956 4976 3197
Size: 965 794 20 4867
Fragments arranged by size:
4867 965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4093 4982 6611
Size: 54 1425 50 275 18 2263 889 1629
Cuts at: 6611 8
Size: 43
Fragments arranged by size:
2263 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2328 2431 2563 4576 1353
Size: 743 232 103 132 2013 3423
Fragments arranged by size:
3423 2013 743 232 132 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 3009 3059 3435 3533 3676 4709
Size: 393 729 648 50 376 98 143 1033
Cuts at: 4709 5306 6093 6361 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1033 787 729 648 597 393 376
268 143 98 50
EcoO109I rG'GnC_Cy
Cuts at: 3079 3079
Size: 6646
EcoRI G'AATT_C
Cuts at: 3640 3931 3955 4333 3640
Size: 291 24 378 5953
Fragments arranged by size:
5953 378 291 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2391 2510 2391
Size: 119 6527
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 3556 5793 1678
Size: 1878 2237 2531
Fragments arranged by size:
2531 2237 1878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 3323 3355 3400
Size: 32 135 32 821 32 898 32 45
Cuts at: 3400 3432 3822 3854 5287 5319 5361 5393 1373
Size: 32 390 32 1433 32 42 32 2626
Fragments arranged by size:
2626 1433 898 821 390 135 45 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2539 3990 4236 669
Size: 988 281 601 1451 246 3079
Fragments arranged by size:
3079 1451 988 601 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 2449 3098 4245 2449
Size: 649 1147 4850
Fragments arranged by size:
4850 1147 649
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2795 3038 5760 5944 1305
Size: 1490 243 2722 184 2007
Fragments arranged by size:
2722 2007 1490 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4824
Fragments arranged by size:
4824 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2130 2631 3693 4604 4963 5122
Size: 648 843 121 501 1062 911 359 159
Cuts at: 5122 518
Size: 2042
Fragments arranged by size:
2042 1062 911 843 648 501 359 159
121
MspA1I CmG'CkG
Cuts at: 1606 3029 3182 4062 4739 5887 6132 1606
Size: 1423 153 880 677 1148 245 2120
Fragments arranged by size:
2120 1423 1148 880 677 245 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5155
Fragments arranged by size:
5155 939 552
NdeI CA'TA_TG
Cuts at: 387 3352 387
Size: 2965 3681
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NsiI A_TGCA'T
Cuts at: 3616 3616
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 3799 4004 5549 1132
Size: 97 2570 205 1545 2229
Fragments arranged by size:
2570 2229 1545 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 3795 5545 3795
Size: 1750 4896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3581 1108
Size: 2473 4173
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PmlI CAC'GTG
Cuts at: 3660 3660
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3079 3079
Size: 6646
PshAI GACnn'nnGTC
Cuts at: 1613 3472 1613
Size: 1859 4787
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PstI C_TGCA'G
Cuts at: 830 2401 3129 3204 3733 3998 830
Size: 1571 728 75 529 265 3478
Fragments arranged by size:
3478 1571 728 529 265 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 3182 3182
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2328 2431 2328
Size: 103 6543
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 2441 3415 3448 4894 6606 2441
Size: 974 33 1446 1712 2481
Fragments arranged by size:
2481 1712 1446 974 33
SexAI A'CCwGG_T
Cuts at: 3580 3580
Size: 6646
SfcI C'TryA_G
Cuts at: 826 1045 2397 2698 2813 3125 3200 3729 3994
Size: 219 1352 301 115 312 75 529 265
Cuts at: 3994 5129 5810 6001 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1352 1135 681 529 312 301 265
219 191 115 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 3267 3556 4750 5651 5913
Size: 197 926 144 1180 289 1194 901 262
Cuts at: 5913 820
Size: 1553
Fragments arranged by size:
1553 1194 1180 926 901 289 262 197
144
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 2594 152
Size: 2442 4204
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 2662 4570 5
Size: 47 2610 1908 2081
Fragments arranged by size:
2610 2081 1908 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6646
StyI C'CwwG_G
Cuts at: 513 1065 2004 4085 513
Size: 552 939 2081 3074
Fragments arranged by size:
3074 2081 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2899 4673 4690 4843 5028 5473 325
Size: 1255 1319 1774 17 153 185 445 1498
Fragments arranged by size:
1774 1498 1319 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2740 3413
Size: 274 80 33 51 1443 462 301 673
Cuts at: 3413 3446 3809 4892 6604 96
Size: 33 363 1083 1712 138
Fragments arranged by size:
1712 1443 1083 673 462 363 301 274
138 80 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6606 6638 6606
Size: 32 6614
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 3935 4775 3935
Size: 840 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI