################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I AspHI AspI AviII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BsaBI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 640 GGTACC G^GTACC AfaI 641 GTAC GT^AC AscI 142 GGCGCGCC GG^CGCGCC Asp718I 640 GGTACC G^GTACC AvrII 801 CCTAGG C^CTAGG BlnI 801 CCTAGG C^CTAGG BlpI 45 GCTGAGC GC^T[ACGT]AGC Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC BsaAI 364 TACGTG [CT]AC^GT[GA] BsaWI 841 ACCGGA [AT]^CCGG[AT] BsiEI 624 CGGCCG CG[GA][CT]^CG BsrBI 55 GAGCGG CCGCTC(-3/-3) BsrI 464 ACTGG ACTGG(1/-1) BssSI 583 CTCGTG CACGAG(-5/-1) CelII 45 GCTGAGC GC^T[ACGT]AGC EagI 624 CGGCCG C^GGCCG EclXI 624 CGGCCG C^GGCCG Eco52I 624 CGGCCG C^GGCCG HincII 925 GTTGAC GT[CT]^[GA]AC HindII 925 GTTGAC GT[CT]^[GA]AC HinfI 845 GAGTC G^A[ACGT]TC KpnI 640 GGTACC GGTAC^C MaeIII 884 GTGAC ^GT[ACGT]AC NaeI 340 GCCGGC GCC^GGC NgoMIV 340 GCCGGC G^CCGGC PflMI 330 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 845 GAGTC GAGTC(4/5) PpuMI 453 GGGTCCC [GA]G^G[AT]CC[CT] RsaI 641 GTAC GT^AC SfaNI 746 GCATC GCATC(5/9) SmlI 616 CTTGAG C^T[CT][GA]AG StuI 359 AGGCCT AGG^CCT Tsp45I 884 GTGAC ^GT[GC]AC TspRI 440 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 330 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 416 CCAGCGTGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 886 GACTGCGTGTC AlwNI 64 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 331 CAGCGCCTG ApaI 271 GGGCCC GGGCC^C 430 GGGCCC AspEI 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 886 GACTGCGTGTC BbsI 822 GAAGAC GAAGAC(2/6) 292 GTCTTC BfaI 802 CTAG C^TAG 856 CTAG BpuAI 822 GAAGAC GAAGAC(2/6) 292 GTCTTC BsaI 127 GAGACC GGTCTC(1/5) 776 GAGACC BsgI 309 CTGCAC GTGCAG(16/14) 469 CTGCAC BspMI 323 ACCTGC ACCTGC(4/8) 560 ACCTGC BssHII 143 GCGCGC G^CGCGC 495 GCGCGC DdeI 46 CTGAG C^T[ACGT]AG 896 CTAAG Eam1105I 724 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 886 GACTGCGTGTC HgaI 28 GACGC GACGC(5/10) 410 GCGTC MaeI 802 CTAG C^TAG 856 CTAG MslI 591 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 762 CACCGTCATG PstI 442 CTGCAG CTGCA^G 562 CTGCAG SfcI 442 CTGCAG C^T[GA][CT]AG 562 CTGCAG TaqI 98 TCGA T^CGA 722 TCGA TthHB8I 98 TCGA T^CGA 722 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV SacII Sau96I ScrFI SfoI StyI TseI