(Circular) (Six-base) MAPSORT of: pFN21AE0296.seq Check: 610 from: 1 to: 5668 pFN21AE0296 5668 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2645 2989 2645 Size: 344 5324 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2515 2846 3731 5115 5383 1632 Size: 883 331 885 1384 268 1917 Fragments arranged by size: 1917 1384 885 883 331 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 2734 2896 4397 2734 Size: 162 1501 4005 Fragments arranged by size: 4005 1501 162 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2074 2341 3411 4983 2074 Size: 267 1070 1572 2759 Fragments arranged by size: 2759 1572 1070 267 ApaI G_GGCC'C Cuts at: 2280 2439 2280 Size: 159 5509 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AscI GG'CGCG_CC Cuts at: 2148 2148 Size: 5668 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2438 2759 2993 1870 Size: 73 210 285 321 234 4545 Fragments arranged by size: 4545 321 285 234 210 73 AvrII C'CTAG_G Cuts at: 2806 2806 Size: 5668 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2282 2348 2594 2645 Size: 325 217 627 463 32 66 246 51 Cuts at: 2645 2810 2989 4444 618 Size: 165 179 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 246 217 179 165 66 51 32 BanII G_rGCy'C Cuts at: 1391 2179 2280 2439 2493 2987 1391 Size: 788 101 159 54 494 4072 Fragments arranged by size: 4072 788 494 159 101 54 BbeI G_GCGC'C Cuts at: 2254 2286 2352 2814 2254 Size: 32 66 462 5108 Fragments arranged by size: 5108 462 66 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 2834 928 Size: 971 391 544 3762 Fragments arranged by size: 3762 971 544 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2063 2165 Size: 107 122 71 631 605 337 54 102 Cuts at: 2165 2346 4279 136 Size: 181 1933 1525 Fragments arranged by size: 1933 1525 631 605 337 181 122 107 102 71 54 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2051 3085 2051 Size: 1034 4634 Bme1580I G_kGCm'C Cuts at: 1790 2068 2280 2439 2597 3729 4885 1790 Size: 278 212 159 158 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 278 212 159 158 BmgBI CAC'GTC Cuts at: 1318 2086 2497 1318 Size: 768 411 4489 Fragments arranged by size: 4489 768 411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2423 2507 4328 1239 Size: 1073 111 84 1821 2579 Fragments arranged by size: 2579 1821 1073 111 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 3793 4658 4956 2642 Size: 1151 865 298 3354 Fragments arranged by size: 3354 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 2775 4331 882 Size: 617 627 649 1556 2219 Fragments arranged by size: 2219 1556 649 627 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 3459 493 Size: 711 1167 1088 2702 Fragments arranged by size: 2702 1167 1088 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2251 2283 2349 Size: 53 83 186 936 501 217 32 66 Cuts at: 2349 2415 2811 3475 3857 4492 275 Size: 66 396 664 382 635 1451 Fragments arranged by size: 1451 936 664 635 501 396 382 217 186 83 66 66 53 32 BsaWI w'CCGG_w Cuts at: 2846 4101 4499 4773 4920 5331 2846 Size: 1255 398 274 147 411 3183 Fragments arranged by size: 3183 1255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5668 BseYI C'CCAG_C Cuts at: 2374 2383 2682 2711 4871 5367 2374 Size: 9 299 29 2160 496 2675 Fragments arranged by size: 2675 2160 496 299 29 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2299 2459 1163 Size: 573 563 160 4372 Fragments arranged by size: 4372 573 563 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2632 3118 3879 4907 1540 Size: 275 186 631 486 761 1028 2301 Fragments arranged by size: 2301 1028 761 631 486 275 186 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2179 2280 2439 2493 2597 2987 Size: 399 278 111 101 159 54 104 390 Cuts at: 2987 3729 3814 4885 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 278 159 111 104 101 85 54 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2337 2574 3023 844 Size: 1493 237 449 3489 Fragments arranged by size: 3489 1493 449 237 BsrBI CCG'CTC Cuts at: 1394 1823 2062 3555 1394 Size: 429 239 1493 3507 Fragments arranged by size: 3507 1493 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2345 2610 4312 4499 1700 Size: 110 211 324 265 1702 187 2869 Fragments arranged by size: 2869 1702 324 265 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 2148 2500 1925 Size: 223 352 5093 Fragments arranged by size: 5093 352 223 BssSI C'ACGA_G Cuts at: 2588 3728 4740 2588 Size: 1140 1012 3516 Fragments arranged by size: 3516 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2179 2271 2394 2409 2667 513 Size: 552 939 175 92 123 15 258 3514 Fragments arranged by size: 3514 939 552 258 175 123 92 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2623 487 Size: 683 56 1397 3532 Fragments arranged by size: 3532 1397 683 56 BtsI GCAGTG_nn' Cuts at: 2444 3184 3978 3998 2444 Size: 740 794 20 4114 Fragments arranged by size: 4114 794 740 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2343 2391 Size: 54 1425 50 275 18 249 264 48 Cuts at: 2391 2629 2676 3115 4004 5633 8 Size: 238 47 439 889 1629 43 Fragments arranged by size: 1629 1425 889 439 275 264 249 238 54 50 48 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2629 3115 1537 Size: 275 817 486 4090 Fragments arranged by size: 4090 817 486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 4255 4629 4775 387 Size: 1064 138 441 2225 374 146 1280 Fragments arranged by size: 2225 1280 1064 441 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2423 2507 2515 2846 3731 4328 Size: 393 680 111 84 8 331 885 597 Cuts at: 4328 5115 5383 1239 Size: 787 268 1524 Fragments arranged by size: 1524 885 787 680 597 393 331 268 111 84 8 EcoO109I rG'GnC_Cy Cuts at: 2158 2276 2435 2459 2554 2158 Size: 118 159 24 95 5272 Fragments arranged by size: 5272 159 118 95 24 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2058 2075 2171 2191 2254 2286 2341 2352 Size: 380 17 96 20 63 32 55 11 Cuts at: 2352 2689 2814 4815 1678 Size: 337 125 2001 2531 Fragments arranged by size: 2531 2001 380 337 125 96 63 55 32 20 17 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 2882 2914 4309 Size: 32 135 32 1148 32 130 32 1395 Cuts at: 4309 4341 4383 4415 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 1148 135 130 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2932 3012 3258 669 Size: 988 281 994 80 246 3079 Fragments arranged by size: 3079 994 988 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KasI G'GCGC_C Cuts at: 2250 2282 2348 2810 2250 Size: 32 66 462 5108 Fragments arranged by size: 5108 462 66 32 KpnI G_GTAC'C Cuts at: 2649 2993 2649 Size: 344 5324 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3846 Fragments arranged by size: 3846 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2771 3626 3985 4144 518 Size: 648 1434 171 855 359 159 2042 Fragments arranged by size: 2042 1434 855 648 359 171 159 MspA1I CmG'CkG Cuts at: 1606 2040 2181 2273 2396 2485 3084 3761 4909 Size: 434 141 92 123 89 599 677 1148 Cuts at: 4909 5154 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 599 434 245 141 123 92 89 NaeI GCC'GGC Cuts at: 2347 2347 Size: 5668 NarI GG'CG_CC Cuts at: 2251 2283 2349 2811 2251 Size: 32 66 462 5108 Fragments arranged by size: 5108 462 66 32 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4177 Fragments arranged by size: 4177 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NgoMIV G'CCGG_C Cuts at: 2345 2345 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2341 1108 Size: 1233 4435 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2459 2459 Size: 5668 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspOMI G'GGCC_C Cuts at: 2276 2435 2276 Size: 159 5509 PstI C_TGCA'G Cuts at: 830 2451 2571 3020 830 Size: 1621 120 449 3478 Fragments arranged by size: 3478 1621 449 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SacII CC_GC'GG Cuts at: 2182 2274 2397 2182 Size: 92 123 5453 Fragments arranged by size: 5453 123 92 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SanDI GG'GwC_CC Cuts at: 2459 2459 Size: 5668 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2447 2567 3016 4151 4832 5023 826 Size: 219 1402 120 449 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 681 449 219 191 120 SfoI GGC'GCC Cuts at: 2252 2284 2350 2812 2252 Size: 32 66 462 5108 Fragments arranged by size: 5108 462 66 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 3772 4673 4935 820 Size: 197 926 678 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2366 1517 Size: 849 4819 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2550 2806 3107 513 Size: 552 939 357 189 256 301 3074 Fragments arranged by size: 3074 939 552 357 301 256 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2428 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI