(Linear) (Six-base) MAPSORT of: KIEE0296.seq Check: 9252 from: 1 to: 963 KIEE0296.orf With 182 enzymes: SgfI * March 2, 2010 14:31 .. Acc65I G'GTAC_C Cuts at: 0 640 963 Size: 640 323 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 510 841 963 Size: 510 331 122 Fragments arranged by size: 510 331 122 AhdI GACnn_n'nnGTC Cuts at: 0 729 891 963 Size: 729 162 72 Fragments arranged by size: 729 162 72 AlwNI CAG_nnn'CTG Cuts at: 0 69 336 963 Size: 69 267 627 Fragments arranged by size: 627 267 69 ApaI G_GGCC'C Cuts at: 0 275 434 963 Size: 275 159 529 Fragments arranged by size: 529 275 159 AscI GG'CGCG_CC Cuts at: 0 143 963 Size: 143 820 AvaI C'yCGr_G Cuts at: 0 148 433 754 963 Size: 148 285 321 209 Fragments arranged by size: 321 285 209 148 AvrII C'CTAG_G Cuts at: 0 801 963 Size: 801 162 BanI G'GyrC_C Cuts at: 0 245 277 343 589 640 805 963 Size: 245 32 66 246 51 165 158 Fragments arranged by size: 246 245 165 158 66 51 32 BanII G_rGCy'C Cuts at: 0 174 275 434 488 963 Size: 174 101 159 54 475 Fragments arranged by size: 475 174 159 101 54 BbeI G_GCGC'C Cuts at: 0 249 281 347 809 963 Size: 249 32 66 462 154 Fragments arranged by size: 462 249 154 66 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 285 829 963 Size: 285 544 134 Fragments arranged by size: 544 285 134 BglI GCCn_nnn'nGGC Cuts at: 0 58 160 341 963 Size: 58 102 181 622 Fragments arranged by size: 622 181 102 58 BlpI GC'TnA_GC Cuts at: 0 46 963 Size: 46 917 Bme1580I G_kGCm'C Cuts at: 0 63 275 434 592 963 Size: 63 212 159 158 371 Fragments arranged by size: 371 212 159 158 63 BmgBI CAC'GTC Cuts at: 0 81 492 963 Size: 81 411 471 Fragments arranged by size: 471 411 81 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 418 502 963 Size: 307 111 84 461 Fragments arranged by size: 461 307 111 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 963 Size: 637 326 BsaI GGTCTCn'nnnn_ Cuts at: 0 121 770 963 Size: 121 649 193 Fragments arranged by size: 649 193 121 BsaAI yAC'GTr Cuts at: 0 366 963 Size: 366 597 BsaHI Gr'CG_yC Cuts at: 0 29 246 278 344 410 806 963 Size: 29 217 32 66 66 396 157 Fragments arranged by size: 396 217 157 66 66 32 29 BsaWI w'CCGG_w Cuts at: 0 841 963 Size: 841 122 BseYI C'CCAG_C Cuts at: 0 369 378 677 706 963 Size: 369 9 299 29 257 Fragments arranged by size: 369 299 257 29 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 454 963 Size: 294 160 509 Fragments arranged by size: 509 294 160 BsiEI CG_ry'CG Cuts at: 0 627 963 Size: 627 336 Bsp1286I G_dGCh'C Cuts at: 0 63 174 275 434 488 592 963 Size: 63 111 101 159 54 104 371 Fragments arranged by size: 371 159 111 104 101 63 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 332 569 963 Size: 332 237 394 Fragments arranged by size: 394 332 237 BsrBI CCG'CTC Cuts at: 0 57 963 Size: 57 906 BsrFI r'CCGG_y Cuts at: 0 16 340 605 963 Size: 16 324 265 358 Fragments arranged by size: 358 324 265 16 BssHII G'CGCG_C Cuts at: 0 143 495 963 Size: 143 352 468 Fragments arranged by size: 468 352 143 BssSI C'ACGA_G Cuts at: 0 583 963 Size: 583 380 BtgI C'CryG_G Cuts at: 0 174 266 389 404 662 963 Size: 174 92 123 15 258 301 Fragments arranged by size: 301 258 174 123 92 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 618 963 Size: 618 345 BtsI GCAGTG_nn' Cuts at: 0 439 963 Size: 439 524 EaeI y'GGCC_r Cuts at: 0 74 338 386 624 671 963 Size: 74 264 48 238 47 292 Fragments arranged by size: 292 264 238 74 48 47 EagI C'GGCC_G Cuts at: 0 624 963 Size: 624 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 25 963 Size: 25 938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 418 502 510 841 963 Size: 307 111 84 8 331 122 Fragments arranged by size: 331 307 122 111 84 8 EcoO109I rG'GnC_Cy Cuts at: 0 153 271 430 454 549 963 Size: 153 118 159 24 95 414 Fragments arranged by size: 414 159 153 118 95 24 HaeII r_GCGC'y Cuts at: 0 53 70 166 186 249 281 336 347 Size: 53 17 96 20 63 32 55 11 Cuts at: 347 684 809 963 Size: 337 125 154 Fragments arranged by size: 337 154 125 96 63 55 53 32 20 17 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 715 747 877 909 963 Size: 715 32 130 32 54 Fragments arranged by size: 715 130 54 32 32 HincII GTy'rAC Cuts at: 0 927 963 Size: 927 36 KasI G'GCGC_C Cuts at: 0 245 277 343 805 963 Size: 245 32 66 462 158 Fragments arranged by size: 462 245 158 66 32 KpnI G_GTAC'C Cuts at: 0 644 963 Size: 644 319 MslI CAynn'nnrTG Cuts at: 0 595 766 963 Size: 595 171 197 Fragments arranged by size: 595 197 171 MspA1I CmG'CkG Cuts at: 0 35 176 268 391 480 963 Size: 35 141 92 123 89 483 Fragments arranged by size: 483 141 123 92 89 35 NaeI GCC'GGC Cuts at: 0 342 963 Size: 342 621 NarI GG'CG_CC Cuts at: 0 246 278 344 806 963 Size: 246 32 66 462 157 Fragments arranged by size: 462 246 157 66 32 NgoMIV G'CCGG_C Cuts at: 0 340 963 Size: 340 623 PflMI CCAn_nnn'nTGG Cuts at: 0 336 963 Size: 336 627 PpuMI rG'GwC_Cy Cuts at: 0 454 963 Size: 454 509 PspOMI G'GGCC_C Cuts at: 0 271 430 963 Size: 271 159 533 Fragments arranged by size: 533 271 159 PstI C_TGCA'G Cuts at: 0 446 566 963 Size: 446 120 397 Fragments arranged by size: 446 397 120 SacII CC_GC'GG Cuts at: 0 177 269 392 963 Size: 177 92 123 571 Fragments arranged by size: 571 177 123 92 SanDI GG'GwC_CC Cuts at: 0 454 963 Size: 454 509 SfcI C'TryA_G Cuts at: 0 442 562 963 Size: 442 120 401 Fragments arranged by size: 442 401 120 SfoI GGC'GCC Cuts at: 0 247 279 345 807 963 Size: 247 32 66 462 156 Fragments arranged by size: 462 247 156 66 32 SmlI C'TyrA_G Cuts at: 0 616 963 Size: 616 347 StuI AGG'CCT Cuts at: 0 361 963 Size: 361 602 StyI C'CwwG_G Cuts at: 0 356 545 801 963 Size: 356 189 256 162 Fragments arranged by size: 356 256 189 162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 423 963 Size: 423 540 Enzymes that do cut: Acc65I AcuI AhdI AlwNI ApaI AscI AvaI AvrII BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BtgI BtgZI BtsI EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI MslI MspA1I NaeI NarI NgoMIV PflMI PpuMI PspOMI PstI SacII SanDI SfcI SfoI SmlI StuI StyI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI ApoI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaBI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI