################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AccI AccII AccIII AclI AflII Alw44I AocI ApaLI AscI AseI AsnI Asp700I AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI Eco81I EcoNI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1098 GGTACC G^GTACC AflIII 728 ACACGT A^C[GA][CT]GT AgeI 1101 ACCGGT A^CCGGT AhdI 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 948 AGCGCT AGC^GCT ApaI 1231 GGGCCC GGGCC^C Asp718I 1098 GGTACC G^GTACC AspEI 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1263 GAGCAC G[AT]GC[AT]^C AspI 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 150 CCCGGG C^[CT]CG[GA]G BamHI 688 GGATCC G^GATCC BclI 374 TGATCA T^GATCA BsiHKAI 1263 GAGCAC G[AT]GC[AT]^C BsmBI 731 CGTCTC CGTCTC(1/5) BsoBI 150 CCCGGG C^[CT]CG[GA]G BsrDI 1183 CATTGC GCAATG(2/0) BssSI 613 CTCGTG CACGAG(-5/-1) BstAPI 1129 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1104 GGTGACC G^GT[ACGT]ACC BstPI 1104 GGTGACC G^GT[ACGT]ACC CfoI 949 GCGC GCG^C DraIII 424 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 247 GACGGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 948 AGCGCT AGC^GCT EcoO65I 1104 GGTGACC G^GT[ACGT]ACC EcoRI 990 GAATTC G^AATTC FbaI 374 TGATCA T^GATCA HaeII 948 AGCGCT [GA]GCGC^[CT] HhaI 949 GCGC GCG^C HinP1I 949 GCGC G^CGC HindIII 221 AAGCTT A^AGCTT KpnI 1098 GGTACC GGTAC^C MspA1I 929 CCGCTG C[AC]G^C[GT]G NheI 1222 GCTAGC G^CTAGC PflFI 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1101 ACCGGT A^CCGGT PleI 598 GACTC GAGTC(4/5) PpuMI 511 AGGACCT [GA]G^G[AT]CC[CT] PshAI 859 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 944 CTGCAG CTGCA^G SexAI 749 ACCAGGT A^CC[AT]GGT SfcI 944 CTGCAG C^T[GA][CT]AG SmaI 150 CCCGGG CCC^GGG SmlI 174 CTTGAG C^T[CT][GA]AG SphI 1073 GCATGC GCATG^C Tth111I 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 150 CCCGGG C^CCGGG XmaI 150 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 253 GACGTC GACGT^C 415 GACGTC AcsI 45 AAATTC [GA]^AATT[CT] 990 GAATTC ApoI 45 AAATTC [GA]^AATT[CT] 990 GAATTC BanII 165 GAGCCC G[GA]GC[CT]^C 1231 GGGCCC BfaI 1111 CTAG C^TAG 1223 CTAG BsaI 106 GAGACC GGTCTC(1/5) 546 GGTCTC BsaWI 921 TCCGGT [AT]^CCGG[AT] 1101 ACCGGT BsiEI 776 CGGCCG CG[GA][CT]^CG 881 CGGCCG DdeI 713 CTGAG C^T[ACGT]AG 983 CTCAG DraII 511 AGGACCT [GA]G^G[ACGT]CC[CT] 1231 GGGCCCC EagI 776 CGGCCG C^GGCCG 881 CGGCCG EarI 624 GAAGAG CTCTTC(1/4) 629 GAAGAG EclXI 776 CGGCCG C^GGCCG 881 CGGCCG Eco52I 776 CGGCCG C^GGCCG 881 CGGCCG EcoO109I 511 AGGACCT [GA]G^G[ACGT]CC[CT] 1231 GGGCCCC EcoT14I 1043 CCATGG C^C[AT][AT]GG 1145 CCATGG FauI 258 CCCGC CCCGC(4/6) 521 CCCGC HgaI 495 GCGTC GACGC(5/10) 1024 GCGTC Ksp632I 624 GAAGAG CTCTTC(1/4) 629 GAAGAG MaeI 1111 CTAG C^TAG 1223 CTAG NaeI 879 GCCGGC GCC^GGC 883 GCCGGC NcoI 1043 CCATGG C^CATGG 1145 CCATGG NgoMIV 879 GCCGGC G^CCGGC 883 GCCGGC NspI 1073 GCATGC [GA]CATG^[CT] 1214 ACATGC PflMI 204 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 341 CCATCTGGTGG StyI 1043 CCATGG C^C[AT][AT]GG 1145 CCATGG TaqI 49 TCGA T^CGA 445 TCGA TfiI 402 GATTC G^A[AT]TC 841 GATTC Tsp509I 46 AATT ^AATT 991 AATT TthHB8I 49 TCGA T^CGA 445 TCGA Van91I 204 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 341 CCATCTGGTGG XcmI 298 CCACAGAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 792 CCAAATCAGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI XhoII