(Circular) (Six-base) MAPSORT of: pFN21AB8316.seq Check: 5049 from: 1 to: 5974 pFN21AB8316 5974 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2262 2424 3784 278 Size: 53 83 186 936 726 162 1360 2468 Fragments arranged by size: 2468 1360 936 726 186 162 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3103 3295 3103 Size: 192 5782 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 5421 5689 1632 Size: 2405 1384 268 1917 Fragments arranged by size: 2405 1917 1384 268 AfeI AGC'GCT Cuts at: 2955 2955 Size: 5974 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 2733 4873 5850 1203 Size: 1530 2140 977 1327 Fragments arranged by size: 2140 1530 1327 977 AgeI A'CCGG_T Cuts at: 3106 4805 3106 Size: 1699 4275 AhdI GACnn_n'nnGTC Cuts at: 2158 4703 2158 Size: 2545 3429 AleI CACnn'nnGTG Cuts at: 1166 2927 1166 Size: 1761 4213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 2216 Size: 32 5942 AlwNI CAG_nnn'CTG Cuts at: 2311 2687 2717 3204 3717 5289 2311 Size: 376 30 487 513 1572 2996 Fragments arranged by size: 2996 1572 513 487 376 30 ApaI G_GGCC'C Cuts at: 3240 3240 Size: 5974 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2050 2995 3283 3509 3661 5770 1493 Size: 557 945 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 945 557 288 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2155 3299 1870 Size: 73 212 1144 4545 Fragments arranged by size: 4545 1144 212 73 BamHI G'GATC_C Cuts at: 2693 3304 3671 2693 Size: 611 367 4996 Fragments arranged by size: 4996 611 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2704 2839 3103 3295 Size: 325 217 627 296 621 135 264 192 Cuts at: 3295 4750 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 621 325 296 264 217 192 135 BanII G_rGCy'C Cuts at: 1391 2174 3240 3293 1391 Size: 783 1066 53 4072 Fragments arranged by size: 4072 1066 783 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2379 1548 Size: 831 5143 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 3240 4035 5191 1790 Size: 1450 795 1156 2573 Fragments arranged by size: 2573 1450 1156 795 BmgBI CAC'GTC Cuts at: 1318 2736 2920 3210 1318 Size: 1418 184 290 4082 Fragments arranged by size: 4082 1418 290 184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 2175 3705 Size: 416 53 83 399 24 675 83 1530 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1530 958 675 416 399 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3231 1056 Size: 2175 3799 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2049 2049 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4634 1239 Size: 3395 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2200 4099 4964 5262 2200 Size: 1899 865 298 2912 Fragments arranged by size: 2912 1899 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2105 2557 4637 882 Size: 617 606 452 2080 2219 Fragments arranged by size: 2219 2080 617 606 452 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 2421 2500 3781 Size: 53 83 186 936 726 162 79 1281 Cuts at: 3781 4163 4798 275 Size: 382 635 1451 Fragments arranged by size: 1451 1281 936 726 635 382 186 162 83 79 53 BsaWI w'CCGG_w Cuts at: 2926 3106 4407 4805 5079 5226 5637 2926 Size: 180 1301 398 274 147 411 3263 Fragments arranged by size: 3263 1301 411 398 274 180 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2606 2684 1186 Size: 1090 330 78 4476 Fragments arranged by size: 4476 1090 330 78 BseYI C'CCAG_C Cuts at: 2125 2880 3125 3172 5177 5673 2125 Size: 755 245 47 2005 496 2426 Fragments arranged by size: 2426 2005 755 496 245 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 3054 3117 3164 3190 1163 Size: 573 901 417 63 47 26 3947 Fragments arranged by size: 3947 901 573 417 63 47 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2784 2889 3424 4185 5213 1540 Size: 275 186 783 105 535 761 1028 2301 Fragments arranged by size: 2301 1028 783 761 535 275 186 105 BsiHKAI G_wGCw'C Cuts at: 3272 4035 4120 5191 3272 Size: 763 85 1071 4055 Fragments arranged by size: 4055 1071 763 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 BsmBI CGTCTCn'nnnn_ Cuts at: 2742 2742 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 3240 3272 3293 4035 4120 5191 Size: 399 384 1066 32 21 742 85 1071 Cuts at: 5191 1391 Size: 2174 Fragments arranged by size: 2174 1071 1066 742 399 384 85 32 21 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3187 4469 4643 65 Size: 1183 1939 1282 174 1396 Fragments arranged by size: 1939 1396 1282 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2779 2783 2884 2888 3106 4618 4805 Size: 110 969 4 101 4 218 1512 187 Cuts at: 4805 1700 Size: 2869 Fragments arranged by size: 2869 1512 969 218 187 110 101 4 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 2618 4034 5046 2618 Size: 1416 1012 3546 Fragments arranged by size: 3546 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3140 794 Size: 2346 3628 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 3109 1184 Size: 1925 4049 BstYI r'GATC_y Cuts at: 1972 2131 2693 2978 3304 3671 4056 4073 5525 Size: 159 562 285 326 367 385 17 1452 Cuts at: 5525 5611 5623 5969 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 562 385 367 346 326 285 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2853 3048 3150 513 Size: 552 939 849 195 102 3337 Fragments arranged by size: 3337 939 849 552 195 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3246 487 Size: 683 56 2020 3215 Fragments arranged by size: 3215 2020 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3490 4284 4304 3202 Size: 288 794 20 4872 Fragments arranged by size: 4872 794 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2434 2434 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 4981 809 Size: 507 942 2723 1802 Fragments arranged by size: 2723 1802 942 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2781 2886 Size: 54 1425 50 275 18 387 564 105 Cuts at: 2886 2931 3421 4310 5939 8 Size: 45 490 889 1629 43 Fragments arranged by size: 1629 1425 889 564 490 387 275 105 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2781 2886 3421 1537 Size: 275 969 105 535 4090 Fragments arranged by size: 4090 969 535 275 105 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 2629 3904 1353 Size: 1271 5 1275 3423 Fragments arranged by size: 3423 1275 1271 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 2839 4561 4935 5081 387 Size: 1064 138 867 383 1722 374 146 1280 Fragments arranged by size: 1722 1280 1064 867 383 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4037 4634 5421 5689 1239 Size: 393 2405 597 787 268 1524 Fragments arranged by size: 2405 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2517 3237 2517 Size: 720 5254 EcoRI G'AATT_C Cuts at: 2995 3283 3661 2995 Size: 288 378 5308 Fragments arranged by size: 5308 378 288 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2103 2479 2103 Size: 376 5598 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2957 5121 1678 Size: 1279 2164 2531 Fragments arranged by size: 2531 2164 1279 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2144 2176 2628 Size: 32 135 32 431 32 109 32 452 Cuts at: 2628 2660 2720 2752 4615 4647 4689 4721 1373 Size: 32 60 32 1863 32 42 32 2626 Fragments arranged by size: 2626 1863 452 431 135 109 60 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 2226 748 Size: 1478 4496 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3107 3299 3107 Size: 192 5782 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2233 5088 5272 1305 Size: 928 2855 184 2007 Fragments arranged by size: 2855 2007 928 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2927 2990 3155 3932 4291 4450 518 Size: 648 1761 63 165 777 359 159 2042 Fragments arranged by size: 2042 1761 777 648 359 165 159 63 MspA1I CmG'CkG Cuts at: 1606 2936 3390 4067 5215 5460 1606 Size: 1330 454 677 1148 245 2120 Fragments arranged by size: 2120 1330 1148 677 454 245 NaeI GCC'GGC Cuts at: 2886 2890 2886 Size: 4 5970 NcoI C'CATG_G Cuts at: 513 1065 2004 3048 3150 513 Size: 552 939 1044 102 3337 Fragments arranged by size: 3337 1044 939 552 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 2884 2888 2884 Size: 4 5970 NheI G'CTAG_C Cuts at: 1052 3227 1052 Size: 2175 3799 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3082 3223 3332 4877 1132 Size: 97 1853 141 109 1545 2229 Fragments arranged by size: 2229 1853 1545 141 109 97 PasI CC'CwG_GG Cuts at: 1348 2269 2942 1348 Size: 921 673 4380 Fragments arranged by size: 4380 921 673 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2215 2352 1108 Size: 1107 137 4730 Fragments arranged by size: 4730 1107 137 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2517 2517 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 2868 1613 Size: 1255 4719 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 3236 3236 Size: 5974 PstI C_TGCA'G Cuts at: 830 2953 3326 830 Size: 2123 373 3478 Fragments arranged by size: 3478 2123 373 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SexAI A'CCwGG_T Cuts at: 2754 2754 Size: 5974 SfcI C'TryA_G Cuts at: 826 1045 2949 3322 4457 5138 5329 826 Size: 219 1904 373 1135 681 191 1471 Fragments arranged by size: 1904 1471 1135 681 373 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 2157 3301 1872 Size: 285 1144 4545 Fragments arranged by size: 4545 1144 285 SmlI C'TyrA_G Cuts at: 820 1017 1943 2179 4078 4979 5241 820 Size: 197 926 236 1899 901 262 1553 Fragments arranged by size: 1899 1553 926 901 262 236 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3082 3332 1132 Size: 1950 250 3774 Fragments arranged by size: 3774 1950 250 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 3048 3150 3413 513 Size: 552 939 1044 102 263 3074 Fragments arranged by size: 3074 1044 939 552 263 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 2630 2810 4001 4018 4171 4356 Size: 1255 559 491 180 1191 17 153 185 Cuts at: 4356 4801 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1191 559 491 445 185 180 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3216 4220 5932 Size: 274 80 33 51 1443 1239 1004 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 1712 1443 1239 1004 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3049 3081 5934 5966 3049 Size: 32 2853 32 3057 Fragments arranged by size: 3057 2853 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2756 1316 Size: 1440 4534 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2310 2804 2310 Size: 494 5480 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 2155 3299 1870 Size: 285 1144 4545 Fragments arranged by size: 4545 1144 285 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2260 2422 3782 276 Size: 53 83 186 936 726 162 1360 2468 Fragments arranged by size: 2468 1360 936 726 186 162 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI