(Linear) (Six-base) MAPSORT of: KIBB8316.seq Check: 5935 from: 1 to: 1269 KIBB8316.orf With 182 enzymes: SgfI * March 2, 2010 14:28 .. AatII G_ACGT'C Cuts at: 0 257 419 1269 Size: 257 162 850 Fragments arranged by size: 850 257 162 Acc65I G'GTAC_C Cuts at: 0 1098 1269 Size: 1098 171 AfeI AGC'GCT Cuts at: 0 950 1269 Size: 950 319 AflIII A'CryG_T Cuts at: 0 728 1269 Size: 728 541 AgeI A'CCGG_T Cuts at: 0 1101 1269 Size: 1101 168 AhdI GACnn_n'nnGTC Cuts at: 0 153 1269 Size: 153 1116 AleI CACnn'nnGTG Cuts at: 0 922 1269 Size: 922 347 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 211 243 1269 Size: 211 32 1026 Fragments arranged by size: 1026 211 32 AlwNI CAG_nnn'CTG Cuts at: 0 306 682 712 1199 1269 Size: 306 376 30 487 70 Fragments arranged by size: 487 376 306 70 30 ApaI G_GGCC'C Cuts at: 0 1235 1269 Size: 1235 34 ApoI r'AATT_y Cuts at: 0 45 990 1269 Size: 45 945 279 Fragments arranged by size: 945 279 45 AvaI C'yCGr_G Cuts at: 0 150 1269 Size: 150 1119 BamHI G'GATC_C Cuts at: 0 688 1269 Size: 688 581 BanI G'GyrC_C Cuts at: 0 78 699 834 1098 1269 Size: 78 621 135 264 171 Fragments arranged by size: 621 264 171 135 78 BanII G_rGCy'C Cuts at: 0 169 1235 1269 Size: 169 1066 34 Fragments arranged by size: 1066 169 34 BclI T'GATC_A Cuts at: 0 374 1269 Size: 374 895 Bme1580I G_kGCm'C Cuts at: 0 1235 1269 Size: 1235 34 BmgBI CAC'GTC Cuts at: 0 731 915 1205 1269 Size: 731 184 290 64 Fragments arranged by size: 731 290 184 64 BmrI ACTGGGnnnn_n' Cuts at: 0 87 170 1269 Size: 87 83 1099 Fragments arranged by size: 1099 87 83 BmtI G_CTAG'C Cuts at: 0 1226 1269 Size: 1226 43 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 44 1269 Size: 44 1225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 1269 Size: 195 1074 BsaI GGTCTCn'nnnn_ Cuts at: 0 100 552 1269 Size: 100 452 717 Fragments arranged by size: 717 452 100 BsaHI Gr'CG_yC Cuts at: 0 254 416 495 1269 Size: 254 162 79 774 Fragments arranged by size: 774 254 162 79 BsaWI w'CCGG_w Cuts at: 0 921 1101 1269 Size: 921 180 168 Fragments arranged by size: 921 180 168 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 601 679 1269 Size: 271 330 78 590 Fragments arranged by size: 590 330 271 78 BseYI C'CCAG_C Cuts at: 0 120 875 1120 1167 1269 Size: 120 755 245 47 102 Fragments arranged by size: 755 245 120 102 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 632 1049 1112 1159 1185 1269 Size: 632 417 63 47 26 84 Fragments arranged by size: 632 417 84 63 47 26 BsiEI CG_ry'CG Cuts at: 0 779 884 1269 Size: 779 105 385 Fragments arranged by size: 779 385 105 BsiHKAI G_wGCw'C Cuts at: 0 1267 1269 Size: 1267 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 737 1269 Size: 737 532 Bsp1286I G_dGCh'C Cuts at: 0 169 1235 1267 1269 Size: 169 1066 32 2 Fragments arranged by size: 1066 169 32 2 BsrDI GCAATG_nn' Cuts at: 0 1182 1269 Size: 1182 87 BsrFI r'CCGG_y Cuts at: 0 774 778 879 883 1101 1269 Size: 774 4 101 4 218 168 Fragments arranged by size: 774 218 168 101 4 4 BssSI C'ACGA_G Cuts at: 0 613 1269 Size: 613 656 BstAPI GCAn_nnn'nTGC Cuts at: 0 1135 1269 Size: 1135 134 BstEII G'GTnAC_C Cuts at: 0 1104 1269 Size: 1104 165 BstYI r'GATC_y Cuts at: 0 126 688 973 1269 Size: 126 562 285 296 Fragments arranged by size: 562 296 285 126 BtgI C'CryG_G Cuts at: 0 848 1043 1145 1269 Size: 848 195 102 124 Fragments arranged by size: 848 195 124 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1241 1269 Size: 1241 28 BtsI GCAGTG_nn' Cuts at: 0 1197 1269 Size: 1197 72 DraIII CAC_nnn'GTG Cuts at: 0 429 1269 Size: 429 840 DrdI GACnn_nn'nnGTC Cuts at: 0 253 1269 Size: 253 1016 EaeI y'GGCC_r Cuts at: 0 212 776 881 926 1269 Size: 212 564 105 45 343 Fragments arranged by size: 564 343 212 105 45 EagI C'GGCC_G Cuts at: 0 776 881 1269 Size: 776 105 388 Fragments arranged by size: 776 388 105 EarI CTCTTCn'nnn_ Cuts at: 0 619 624 1269 Size: 619 5 645 Fragments arranged by size: 645 619 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 834 1269 Size: 451 383 435 Fragments arranged by size: 451 435 383 EcoO109I rG'GnC_Cy Cuts at: 0 512 1232 1269 Size: 512 720 37 Fragments arranged by size: 720 512 37 EcoRI G'AATT_C Cuts at: 0 990 1269 Size: 990 279 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 98 474 1269 Size: 98 376 795 Fragments arranged by size: 795 376 98 HaeII r_GCGC'y Cuts at: 0 952 1269 Size: 952 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 30 139 171 623 655 715 747 1269 Size: 30 109 32 452 32 60 32 522 Fragments arranged by size: 522 452 109 60 32 32 32 30 HindIII A'AGCT_T Cuts at: 0 221 1269 Size: 221 1048 KpnI G_GTAC'C Cuts at: 0 1102 1269 Size: 1102 167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 228 1269 Size: 228 1041 MslI CAynn'nnrTG Cuts at: 0 922 985 1150 1269 Size: 922 63 165 119 Fragments arranged by size: 922 165 119 63 MspA1I CmG'CkG Cuts at: 0 931 1269 Size: 931 338 NaeI GCC'GGC Cuts at: 0 881 885 1269 Size: 881 4 384 Fragments arranged by size: 881 384 4 NcoI C'CATG_G Cuts at: 0 1043 1145 1269 Size: 1043 102 124 Fragments arranged by size: 1043 124 102 NgoMIV G'CCGG_C Cuts at: 0 879 883 1269 Size: 879 4 386 Fragments arranged by size: 879 386 4 NheI G'CTAG_C Cuts at: 0 1222 1269 Size: 1222 47 NspI r_CATG'y Cuts at: 0 1077 1218 1269 Size: 1077 141 51 Fragments arranged by size: 1077 141 51 PasI CC'CwG_GG Cuts at: 0 264 937 1269 Size: 264 673 332 Fragments arranged by size: 673 332 264 PflMI CCAn_nnn'nTGG Cuts at: 0 210 347 1269 Size: 210 137 922 Fragments arranged by size: 922 210 137 PpuMI rG'GwC_Cy Cuts at: 0 512 1269 Size: 512 757 PshAI GACnn'nnGTC Cuts at: 0 863 1269 Size: 863 406 PspOMI G'GGCC_C Cuts at: 0 1231 1269 Size: 1231 38 PstI C_TGCA'G Cuts at: 0 948 1269 Size: 948 321 SexAI A'CCwGG_T Cuts at: 0 749 1269 Size: 749 520 SfcI C'TryA_G Cuts at: 0 944 1269 Size: 944 325 SmaI CCC'GGG Cuts at: 0 152 1269 Size: 152 1117 SmlI C'TyrA_G Cuts at: 0 174 1269 Size: 174 1095 SphI G_CATG'C Cuts at: 0 1077 1269 Size: 1077 192 StyI C'CwwG_G Cuts at: 0 1043 1145 1269 Size: 1043 102 124 Fragments arranged by size: 1043 124 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 134 625 805 1269 Size: 134 491 180 464 Fragments arranged by size: 491 464 180 134 TatI w'GTAC_w Cuts at: 0 1211 1269 Size: 1211 58 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1044 1076 1269 Size: 1044 32 193 Fragments arranged by size: 1044 193 32 Tth111I GACn'n_nGTC Cuts at: 0 751 1269 Size: 751 518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 305 799 1269 Size: 305 494 470 Fragments arranged by size: 494 470 305 XmaI C'CCGG_G Cuts at: 0 150 1269 Size: 150 1119 ZraI GAC'GTC Cuts at: 0 255 417 1269 Size: 255 162 852 Fragments arranged by size: 852 255 162 Enzymes that do cut: AatII Acc65I AfeI AflIII AgeI AhdI AleI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BclI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI PpuMI PshAI PspOMI PstI SexAI SfcI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AcuI AflII AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BpmI Bpu10I BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI EcoICRI Eco57MI EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI MfeI MluI MscI NarI NdeI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI