################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 568 GTCTAC GT^[AC][GT]AC AcyI 295 GGCGCC G[GA]^CG[CT]C AlwI 533 GGATC GGATC(4/5) AlwNI 576 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 550 GGGCCC GGGCC^C AspHI 706 GTGCTC G[AT]GC[AT]^C AvaI 66 CCCGAG C^[CT]CG[GA]G BalI 234 TGGCCA TGG^CCA BbeI 295 GGCGCC GGCGC^C BbvCI 824 CCTCAGC CCTCAGC(-5/-2) BcgI 716 GCAGCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 295 GGCGCC G[GA]^CG[CT]C BseRI 768 CTCCTC GAGGAG(10/8) BsgI 389 GTGCAG GTGCAG(16/14) BsiHKAI 706 GTGCTC G[AT]GC[AT]^C BsmAI 901 GTCTC GTCTC(1/5) BsmBI 900 CGTCTC CGTCTC(1/5) BsoBI 66 CCCGAG C^[CT]CG[GA]G BsrDI 223 GCAATG GCAATG(2/0) BsrFI 540 GCCGGC [GA]^CCGG[CT] BstEII 282 GGTAACC G^GT[ACGT]ACC BstPI 282 GGTAACC G^GT[ACGT]ACC Cfr10I 540 GCCGGC [GA]^CCGG[CT] DpnII 534 GATC ^GATC EagI 44 CGGCCG C^GGCCG EarI 835 CTCTTC CTCTTC(1/4) EclXI 44 CGGCCG C^GGCCG Eco52I 44 CGGCCG C^GGCCG EcoNI 918 CCTTATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 282 GGTAACC G^GT[ACGT]ACC FauI 73 CCCGC CCCGC(4/6) Hin1I 295 GGCGCC G[GA]^CG[CT]C KasI 295 GGCGCC G^GCGCC Ksp632I 835 CTCTTC CTCTTC(1/4) MboI 534 GATC ^GATC MluNI 234 TGGCCA TGG^CCA MscI 234 TGGCCA TGG^CCA MseI 190 TTAA T^TAA MslI 132 CACGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 540 GCCGGC GCC^GGC NarI 295 GGCGCC GG^CGCC NcoI 546 CCATGG C^CATGG NdeII 534 GATC ^GATC NgoMIV 540 GCCGGC G^CCGGC PvuII 11 CAGCTG CAG^CTG Sau3AI 534 GATC ^GATC SfoI 295 GGCGCC GGC^GCC TfiI 890 GATTC G^A[AT]TC Tru9I 190 TTAA T^TAA Tsp45I 436 GTGAC ^GT[GC]AC Tsp509I 943 AATT ^AATT TspRI 476 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 554 CCACCAACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 59 CGCG CG^CG 537 CGCG BanI 295 GGCGCC G^G[CT][GA]CC 374 GGTGCC BcnI 47 CCGGG CC^[GC]GG 840 CCGGG BlpI 381 GCTAAGC GC^T[ACGT]AGC 448 GCTGAGC BpmI 454 CTGGAG CTGGAG(16/14) 754 CTGGAG Bpu1102I 381 GCTAAGC GC^T[ACGT]AGC 448 GCTGAGC BsiEI 44 CGGCCG CG[GA][CT]^CG 896 CGACCG BsmFI 4 GGGAC GGGAC(10/14) 268 GTCCC BstF5I 831 CATCC GGATG(2/0) 878 GGATG BstUI 59 CGCG CG^CG 537 CGCG BstXI 272 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 557 CCAACATCATGG CelII 381 GCTAAGC GC^T[ACGT]AGC 448 GCTGAGC FokI 831 CATCC GGATG(9/13) 878 GGATG HaeII 295 GGCGCC [GA]GCGC^[CT] 750 GGCGCT HphI 120 GGTGA GGTGA(8/7) 602 TCACC MaeIII 283 GTAAC ^GT[ACGT]AC 436 GTGAC MspA1I 11 CAGCTG C[AC]G^C[GT]G 20 CAGCGG MvnI 59 CGCG CG^CG 537 CGCG NciI 47 CCGGG CC^[GC]GG 840 CCGGG PleI 441 GACTC GAGTC(4/5) 766 GACTC PstI 8 CTGCAG CTGCA^G 714 CTGCAG TaqI 112 TCGA T^CGA 764 TCGA TthHB8I 112 TCGA T^CGA 764 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboII MnlI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI StyI TseI