(Circular) (Six-base) MAPSORT of: pFN21AE0293.seq Check: 5129 from: 1 to: 5692 pFN21AE0293 5692 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 3035 1937 Size: 637 461 4594 Fragments arranged by size: 4594 637 461 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 3755 5139 5407 1632 Size: 734 1389 1384 268 1917 Fragments arranged by size: 1917 1389 1384 734 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 2141 1166 Size: 975 4717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2525 1184 Size: 1341 4351 AlwNI CAG_nnn'CTG Cuts at: 2586 3435 5007 2586 Size: 849 1572 3271 Fragments arranged by size: 3271 1572 849 ApaI G_GGCC'C Cuts at: 2559 2559 Size: 5692 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2071 3017 1870 Size: 73 128 946 4545 Fragments arranged by size: 4545 946 128 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2379 3013 4468 618 Size: 325 217 627 513 79 634 1455 1842 Fragments arranged by size: 1842 1455 634 627 513 325 217 79 BanII G_rGCy'C Cuts at: 1391 2078 2559 2683 3011 1391 Size: 687 481 124 328 4072 Fragments arranged by size: 4072 687 481 328 124 BbeI G_GCGC'C Cuts at: 2304 2304 Size: 5692 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4721 BbvCI CC'TCA_GC Cuts at: 2830 2830 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2710 2744 3883 3917 1903 Size: 34 773 34 1139 34 3678 Fragments arranged by size: 3678 1139 773 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 2387 2454 3109 2387 Size: 67 655 4970 Fragments arranged by size: 4970 655 67 Bme1580I G_kGCm'C Cuts at: 1790 2559 3753 4909 1790 Size: 769 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 769 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2474 3423 4381 Size: 416 53 83 399 24 1057 949 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1057 958 949 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 2780 4352 1239 Size: 1241 300 1572 2579 Fragments arranged by size: 2579 1572 1241 300 Bpu10I CC'TnA_GC Cuts at: 2830 2830 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 3499 3881 4516 Size: 53 83 186 936 768 1198 382 635 Cuts at: 4516 275 Size: 1451 Fragments arranged by size: 1451 1198 936 768 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5467 Fragments arranged by size: 5467 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2764 1186 Size: 1578 4114 BseYI C'CCAG_C Cuts at: 2596 4895 5391 2596 Size: 2299 496 2897 Fragments arranged by size: 2897 2299 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2415 1163 Size: 573 679 4440 Fragments arranged by size: 4440 679 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2904 3142 3903 4931 1540 Size: 275 186 51 852 238 761 1028 2301 Fragments arranged by size: 2301 1028 852 761 275 238 186 51 BsiHKAI G_wGCw'C Cuts at: 2715 3753 3838 4909 2715 Size: 1038 85 1071 3498 Fragments arranged by size: 3498 1071 1038 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 BsmBI CGTCTCn'nnnn_ Cuts at: 2911 2911 Size: 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2559 2683 2715 3011 3753 3838 Size: 399 288 481 124 32 296 742 85 Cuts at: 3838 4909 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 481 399 296 288 124 85 32 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3047 844 Size: 2203 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2235 4187 4361 65 Size: 1183 987 1952 174 1396 Fragments arranged by size: 1952 1396 1183 987 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2545 4336 4523 1700 Size: 110 735 1791 187 2869 Fragments arranged by size: 2869 1791 735 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 2287 1184 Size: 1103 4589 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2569 2284 Size: 285 5407 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 2551 2910 513 Size: 552 939 273 274 359 3295 Fragments arranged by size: 3295 939 552 359 274 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 3208 4002 4022 3208 Size: 794 20 4878 Fragments arranged by size: 4878 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2106 2239 Size: 54 1425 50 275 18 219 57 133 Cuts at: 2239 2706 3139 4028 5657 8 Size: 467 433 889 1629 43 Fragments arranged by size: 1629 1425 889 467 433 275 219 133 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2049 3139 1537 Size: 275 237 1090 4090 Fragments arranged by size: 4090 1090 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 2846 3622 1353 Size: 1493 776 3423 Fragments arranged by size: 3423 1493 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2480 2780 3755 4352 5139 5407 Size: 393 734 114 300 975 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 975 787 734 597 393 300 268 114 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2927 Size: 5692 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 2304 2759 4839 1678 Size: 626 455 2080 2531 Fragments arranged by size: 2531 2080 626 455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4333 4365 4407 4439 1373 Size: 32 135 32 2761 32 42 32 2626 Fragments arranged by size: 2761 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KasI G'GCGC_C Cuts at: 2300 2300 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 10 Size: 54 1425 343 409 3461 Fragments arranged by size: 3461 1425 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 3650 4009 4168 518 Size: 648 975 1509 359 159 2042 Fragments arranged by size: 2042 1509 975 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2027 3108 3785 4933 5178 1606 Size: 412 9 1081 677 1148 245 2120 Fragments arranged by size: 2120 1148 1081 677 412 245 9 NaeI GCC'GGC Cuts at: 2547 2547 Size: 5692 NarI GG'CG_CC Cuts at: 2301 2301 Size: 5692 NcoI C'CATG_G Cuts at: 513 1065 2004 2551 513 Size: 552 939 547 3654 Fragments arranged by size: 3654 939 552 547 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NgoMIV G'CCGG_C Cuts at: 2545 2545 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 2296 1348 Size: 948 4744 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PfoI T'CCnGG_A Cuts at: 2844 2844 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2555 2555 Size: 5692 PstI C_TGCA'G Cuts at: 830 2017 2723 3044 830 Size: 1187 706 321 3478 Fragments arranged by size: 3478 1187 706 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2018 2018 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2013 2147 2719 3040 4175 4856 5047 Size: 219 968 134 572 321 1135 681 191 Cuts at: 5047 826 Size: 1471 Fragments arranged by size: 1471 1135 968 681 572 321 219 191 134 SfoI GGC'GCC Cuts at: 2302 2302 Size: 5692 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2004 2305 2551 2622 2956 3131 513 Size: 552 939 301 246 71 334 175 3074 Fragments arranged by size: 3074 939 552 334 301 246 175 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2875 2907 5652 5684 2875 Size: 32 2745 32 2883 Fragments arranged by size: 2883 2745 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2566 2566 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI