Restriction map for
pFN21AA0005
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoT14I FauI FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NcoI NdeI NgoMIV NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcsI 618 AAATTT [GA]^AATT[CT]
AcyI 9 GGCGCC G[GA]^CG[CT]C
AflIII 1248 ACATGT A^C[GA][CT]GT
AgeI 223 ACCGGT A^CCGGT
AlwI 901 GATCC GGATC(4/5)
ApaI 17 GGGCCC GGGCC^C
ApoI 618 AAATTT [GA]^AATT[CT]
AvaII 48 GGTCC G^G[AT]CC
BanI 9 GGCGCC G^G[CT][GA]CC
BbeI 9 GGCGCC GGCGC^C
BbsI 710 GTCTTC GAAGAC(2/6)
BcnI 21 CCCGG CC^[GC]GG
BglII 402 AGATCT A^GATCT
BpuAI 710 GTCTTC GAAGAC(2/6)
BsaHI 9 GGCGCC G[GA]^CG[CT]C
BsaI 409 GAGACC GGTCTC(1/5)
BsaWI 223 ACCGGT [AT]^CCGG[AT]
BsiEI 64 CGGCCG CG[GA][CT]^CG
BsiYI 1209 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1209 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 409 GAGAC GTCTC(1/5)
BsmFI 891 GTCCC GGGAC(10/14)
BsmI 329 GAATGC GAATGC(1/-1)
BspHI 1160 TCATGA T^CATGA
BspLU11I 1248 ACATGT A^CATGT
BsrBI 51 CCGCTC CCGCTC(-3/-3)
BsrFI 223 ACCGGT [GA]^CCGG[CT]
BstYI 402 AGATCT [GA]^GATC[CT]
Cfr10I 223 ACCGGT [GA]^CCGG[CT]
DrdI 81 GACAGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 894 CCGTGG C^C[GA][CT]GG
EaeI 64 CGGCCG [CT]^GGCC[GA]
EagI 64 CGGCCG C^GGCCG
EarI 1263 GAAGAG CTCTTC(1/4)
EclXI 64 CGGCCG C^GGCCG
Eco52I 64 CGGCCG C^GGCCG
EcoNI 1209 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 688 GATATC GAT^ATC
EcoT22I 587 ATGCAT ATGCA^T
Hin1I 9 GGCGCC G[GA]^CG[CT]C
HincII 552 GTTGAC GT[CT]^[GA]AC
HindII 552 GTTGAC GT[CT]^[GA]AC
KasI 9 GGCGCC G^GCGCC
Ksp632I 1263 GAAGAG CTCTTC(1/4)
MflI 402 AGATCT [GA]^GATC[CT]
MslI 312 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 9 GGCGCC GG^CGCC
NciI 21 CCCGG CC^[GC]GG
NheI 543 GCTAGC G^CTAGC
NotI 63 GCGGCCGC GC^GGCCGC
NsiI 587 ATGCAT ATGCA^T
NspI 1248 ACATGT [GA]CATG^[CT]
PinAI 223 ACCGGT A^CCGGT
PshAI 42 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1160 TCATGA T^CATGA
SfoI 9 GGCGCC GGC^GCC
SspI 788 AATATT AAT^ATT
TaqI 288 TCGA T^CGA
TthHB8I 288 TCGA T^CGA
XbaI 405 TCTAGA T^CTAGA
XhoII 402 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 38 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
698 CAGTAGCTG
BstF5I 590 CATCC GGATG(2/0)
954 CATCC
DpnII 403 GATC ^GATC
901 GATC
DraI 142 TTTAAA TTT^AAA
664 TTTAAA
DraII 16 GGGGCCC [GA]G^G[ACGT]CC[CT]
17 GGGCCCC
EcoO109I 16 GGGGCCC [GA]G^G[ACGT]CC[CT]
17 GGGCCCC
FokI 590 CATCC GGATG(9/13)
954 CATCC
HapII 22 CCGG C^CGG
224 CCGG
HpaII 22 CCGG C^CGG
224 CCGG
HphI 898 GGTGA GGTGA(8/7)
1348 GGTGA
MboI 403 GATC ^GATC
901 GATC
MspI 22 CCGG C^CGG
224 CCGG
NdeII 403 GATC ^GATC
901 GATC
PleI 1113 GACTC GAGTC(4/5)
526 GAGTC
SacI 851 GAGCTC GAGCT^C
862 GAGCTC
Sau3AI 403 GATC ^GATC
901 GATC
SmlI 1094 CTCAAG C^T[CT][GA]AG
1276 CTTGAG
Tsp45I 40 GTGAC ^GT[GC]AC
1349 GTGAC
Tsp509I 619 AATT ^AATT
918 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI Bsp1286I BsrI
BssKI BstAPI BstNI Cac8I CfoI
Cfr13I DdeI EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I HinfI ItaI
MaeI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI TspRI XmnI