(Circular) (Six-base) MAPSORT of: pFN21AA0005.seq Check: 1256 from: 1 to: 6058 pFN21AA0005 6058 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3149 3228 3342 3360 3366 4121 5505 5773 Size: 1517 79 114 18 6 755 1384 268 Cuts at: 5773 1632 Size: 1917 Fragments arranged by size: 1917 1517 1384 755 268 114 79 18 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 3253 4957 5934 1203 Size: 2050 1704 977 1327 Fragments arranged by size: 2050 1704 1327 977 AgeI A'CCGG_T Cuts at: 2228 4889 2228 Size: 2661 3397 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2445 1184 Size: 1261 4797 AlwNI CAG_nnn'CTG Cuts at: 2048 2708 3801 5373 2048 Size: 660 1093 1572 2733 Fragments arranged by size: 2733 1572 1093 660 ApaI G_GGCC'C Cuts at: 2026 2026 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 2623 3367 3593 3745 5854 1493 Size: 1130 744 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 3383 1870 Size: 73 1440 4545 Fragments arranged by size: 4545 1440 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 3379 4834 618 Size: 325 217 627 227 1365 1455 1842 Fragments arranged by size: 1842 1455 1365 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2026 2860 2871 3228 3377 1391 Size: 635 834 11 357 149 4072 Fragments arranged by size: 4072 834 635 357 149 11 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 6058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2708 928 Size: 971 809 4278 Fragments arranged by size: 4278 971 809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BfrBI ATG'CAT Cuts at: 2594 2594 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2407 6053 2407 Size: 3646 2412 BlpI GC'TnA_GC Cuts at: 3475 3475 Size: 6058 Bme1580I G_kGCm'C Cuts at: 1790 2026 4119 5275 1790 Size: 236 2093 1156 2573 Fragments arranged by size: 2573 2093 1156 236 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3789 4747 442 Size: 416 53 83 399 24 2372 958 1753 Fragments arranged by size: 2372 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2552 1056 Size: 1496 4562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 2492 2855 4718 1239 Size: 1053 200 363 1863 2579 Fragments arranged by size: 2579 1863 1053 363 200 Bpu10I CC'TnA_GC Cuts at: 2433 3215 2433 Size: 782 5276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3084 3302 4183 5048 5346 3084 Size: 218 881 865 298 3796 Fragments arranged by size: 3796 881 865 298 218 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 4721 882 Size: 617 909 2313 2219 Fragments arranged by size: 2313 2219 909 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3865 4247 4882 Size: 53 83 186 936 482 1850 382 635 Cuts at: 4882 275 Size: 1451 Fragments arranged by size: 1850 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2228 4491 4889 5163 5310 5721 2228 Size: 2263 398 274 147 411 2565 Fragments arranged by size: 2565 2263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5833 Fragments arranged by size: 5833 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3044 3236 1186 Size: 1768 90 192 4008 Fragments arranged by size: 4008 1768 192 90 BseYI C'CCAG_C Cuts at: 5261 5757 5261 Size: 496 5562 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2072 3508 4269 5297 1540 Size: 275 186 71 1436 761 1028 2301 Fragments arranged by size: 2301 1436 1028 761 275 186 71 BsiHKAI G_wGCw'C Cuts at: 2860 2871 3008 4119 4204 5275 2860 Size: 11 137 1111 85 1071 3643 Fragments arranged by size: 3643 1111 1071 137 85 11 BsmI GAATG_Cn' Cuts at: 2340 3569 3662 2340 Size: 1229 93 4736 Fragments arranged by size: 4736 1229 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2860 2871 3008 3228 3377 4119 Size: 399 236 834 11 137 220 149 742 Cuts at: 4119 4204 5275 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 834 742 399 236 220 149 137 85 11 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3165 3947 5677 3165 Size: 782 1730 3546 Fragments arranged by size: 3546 1730 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3413 844 Size: 2569 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2058 3945 1394 Size: 429 235 1887 3507 Fragments arranged by size: 3507 1887 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 4727 65 Size: 1183 3305 174 1396 Fragments arranged by size: 3305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 4702 4889 1700 Size: 110 418 2474 187 2869 Fragments arranged by size: 2869 2474 418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 2654 2708 3257 794 Size: 1860 54 549 3595 Fragments arranged by size: 3595 1860 549 54 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstYI r'GATC_y Cuts at: 1972 2407 3388 3755 4140 4157 5609 5695 5707 Size: 435 981 367 385 17 1452 86 12 Cuts at: 5707 6053 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 435 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2899 513 Size: 552 1834 3672 Fragments arranged by size: 3672 1834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3574 4368 4388 3574 Size: 794 20 5244 Fragments arranged by size: 5244 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 2149 2671 3362 2149 Size: 522 691 4845 Fragments arranged by size: 4845 691 522 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2092 5065 809 Size: 507 776 2973 1802 Fragments arranged by size: 2973 1802 776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2069 3505 4394 Size: 54 1425 50 275 18 239 1436 889 Cuts at: 4394 6023 8 Size: 1629 43 Fragments arranged by size: 1629 1436 1425 889 275 239 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2069 3505 1537 Size: 275 257 1436 4090 Fragments arranged by size: 4090 1436 275 257 EarI CTCTTCn'nnn_ Cuts at: 1353 3263 3988 1353 Size: 1910 725 3423 Fragments arranged by size: 3423 1910 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2858 2869 2858 Size: 11 6047 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2492 2855 3149 3228 3342 3360 Size: 393 660 200 363 294 79 114 18 Cuts at: 3360 3366 4121 4718 5505 5773 1239 Size: 6 755 597 787 268 1524 Fragments arranged by size: 1524 787 755 660 597 393 363 294 268 200 114 79 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 3218 3218 Size: 6058 EcoO109I rG'GnC_Cy Cuts at: 2022 2023 2022 Size: 1 6057 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 EcoRV GAT'ATC Cuts at: 2695 2695 Size: 6058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 3050 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2018 2040 2461 5205 1678 Size: 340 22 421 2744 2531 Fragments arranged by size: 2744 2531 421 340 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373 Size: 32 135 32 3127 32 42 32 2626 Fragments arranged by size: 3127 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2559 3402 3648 669 Size: 988 281 621 843 246 3079 Fragments arranged by size: 3079 988 843 621 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KasI G'GCGC_C Cuts at: 2014 2014 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 5172 5356 1305 Size: 1209 2658 184 2007 Fragments arranged by size: 2658 2007 1209 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4236 Fragments arranged by size: 4236 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 4016 4375 4534 518 Size: 648 1155 1695 359 159 2042 Fragments arranged by size: 2042 1695 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3474 4151 5299 5544 1606 Size: 1868 677 1148 245 2120 Fragments arranged by size: 2120 1868 1148 677 245 NarI GG'CG_CC Cuts at: 2015 2015 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5506 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NheI G'CTAG_C Cuts at: 1052 2548 1052 Size: 1496 4562 NotI GC'GGCC_GC Cuts at: 2069 3505 2069 Size: 1436 4622 NsiI A_TGCA'T Cuts at: 2596 2596 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 3257 3416 4961 1132 Size: 97 2028 159 1545 2229 Fragments arranged by size: 2229 2028 1545 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 3253 4957 3253 Size: 1704 4354 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6058 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2051 1613 Size: 438 5620 PsiI TTA'TAA Cuts at: 2616 3201 3628 2616 Size: 585 427 5046 Fragments arranged by size: 5046 585 427 PspOMI G'GGCC_C Cuts at: 2022 2022 Size: 6058 PstI C_TGCA'G Cuts at: 830 3410 830 Size: 2580 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 SacI G_AGCT'C Cuts at: 2860 2871 2860 Size: 11 6047 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 3406 4541 5222 5413 826 Size: 219 2361 1135 681 191 1471 Fragments arranged by size: 2361 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2016 2016 Size: 6058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 3385 1872 Size: 1513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3099 3281 4162 5063 5325 820 Size: 197 926 1156 182 881 901 262 1553 Fragments arranged by size: 1553 1156 926 901 881 262 197 182 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 2795 3982 5 Size: 47 2743 1187 2081 Fragments arranged by size: 2743 2081 1187 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 3497 513 Size: 552 2432 3074 Fragments arranged by size: 3074 2432 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4085 4102 4255 4440 4885 325 Size: 1255 2505 17 153 185 445 1498 Fragments arranged by size: 2505 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4304 6016 96 Size: 274 80 33 51 1443 2327 1712 138 Fragments arranged by size: 2327 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6018 6050 6018 Size: 32 6026 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 2410 3394 2410 Size: 984 5074 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 3383 1870 Size: 1513 4545 XmnI GAAnn'nnTTC Cuts at: 2754 2832 3173 4187 2754 Size: 78 341 1014 4625 Fragments arranged by size: 4625 1014 341 78 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI