Restriction map for
pFN21AA0005
(Linear) (Six-base) MAPSORT of: KIAA0005.seq Check: 8358 from: 1 to: 1353
KIAA0005.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1223 1337 1353
Size: 1144 79 114 16
Fragments arranged by size:
1144 114 79 16
AflIII A'CryG_T
Cuts at: 0 1248 1353
Size: 1248 105
AgeI A'CCGG_T
Cuts at: 0 223 1353
Size: 223 1130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 440 1353
Size: 440 913
AlwNI CAG_nnn'CTG
Cuts at: 0 43 703 1353
Size: 43 660 650
Fragments arranged by size:
660 650 43
ApaI G_GGCC'C
Cuts at: 0 21 1353
Size: 21 1332
ApoI r'AATT_y
Cuts at: 0 618 1353
Size: 618 735
BanI G'GyrC_C
Cuts at: 0 9 1353
Size: 9 1344
BanII G_rGCy'C
Cuts at: 0 21 855 866 1223 1353
Size: 21 834 11 357 130
Fragments arranged by size:
834 357 130 21 11
BbeI G_GCGC'C
Cuts at: 0 13 1353
Size: 13 1340
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 1353
Size: 703 650
BfrBI ATG'CAT
Cuts at: 0 589 1353
Size: 589 764
BglII A'GATC_T
Cuts at: 0 402 1353
Size: 402 951
Bme1580I G_kGCm'C
Cuts at: 0 21 1353
Size: 21 1332
BmtI G_CTAG'C
Cuts at: 0 547 1353
Size: 547 806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 487 850 1353
Size: 287 200 363 503
Fragments arranged by size:
503 363 287 200
Bpu10I CC'TnA_GC
Cuts at: 0 428 1210 1353
Size: 428 782 143
Fragments arranged by size:
782 428 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1297 1353
Size: 1079 218 56
Fragments arranged by size:
1079 218 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 1353
Size: 403 950
BsaHI Gr'CG_yC
Cuts at: 0 10 1353
Size: 10 1343
BsaWI w'CCGG_w
Cuts at: 0 223 1353
Size: 223 1130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1039 1231 1353
Size: 949 90 192 122
Fragments arranged by size:
949 192 122 90
BsiEI CG_ry'CG
Cuts at: 0 67 1353
Size: 67 1286
BsiHKAI G_wGCw'C
Cuts at: 0 855 866 1003 1353
Size: 855 11 137 350
Fragments arranged by size:
855 350 137 11
BsmI GAATG_Cn'
Cuts at: 0 335 1353
Size: 335 1018
Bsp1286I G_dGCh'C
Cuts at: 0 21 855 866 1003 1223 1353
Size: 21 834 11 137 220 130
Fragments arranged by size:
834 220 137 130 21 11
BspHI T'CATG_A
Cuts at: 0 1160 1353
Size: 1160 193
BsrBI CCG'CTC
Cuts at: 0 53 1353
Size: 53 1300
BsrFI r'CCGG_y
Cuts at: 0 223 1353
Size: 223 1130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 649 703 1252 1353
Size: 649 54 549 101
Fragments arranged by size:
649 549 101 54
BstYI r'GATC_y
Cuts at: 0 402 1353
Size: 402 951
BtgI C'CryG_G
Cuts at: 0 894 1353
Size: 894 459
DraI TTT'AAA
Cuts at: 0 144 666 1353
Size: 144 522 687
Fragments arranged by size:
687 522 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 87 1353
Size: 87 1266
EaeI y'GGCC_r
Cuts at: 0 64 1353
Size: 64 1289
EagI C'GGCC_G
Cuts at: 0 64 1353
Size: 64 1289
EarI CTCTTCn'nnn_
Cuts at: 0 1258 1353
Size: 1258 95
EcoICRI GAG'CTC
Cuts at: 0 853 864 1353
Size: 853 11 489
Fragments arranged by size:
853 489 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 487 850 1144 1223 1337 1353
Size: 287 200 363 294 79 114 16
Fragments arranged by size:
363 294 287 200 114 79 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1213 1353
Size: 1213 140
EcoO109I rG'GnC_Cy
Cuts at: 0 17 18 1353
Size: 17 1 1335
Fragments arranged by size:
1335 17 1
EcoRV GAT'ATC
Cuts at: 0 690 1353
Size: 690 663
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1045 1353
Size: 1045 308
HaeII r_GCGC'y
Cuts at: 0 13 35 456 1353
Size: 13 22 421 897
Fragments arranged by size:
897 421 22 13
HincII GTy'rAC
Cuts at: 0 554 1353
Size: 554 799
KasI G'GCGC_C
Cuts at: 0 9 1353
Size: 9 1344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 1353
Size: 509 844
MslI CAynn'nnrTG
Cuts at: 0 316 1353
Size: 316 1037
NarI GG'CG_CC
Cuts at: 0 10 1353
Size: 10 1343
NheI G'CTAG_C
Cuts at: 0 543 1353
Size: 543 810
NotI GC'GGCC_GC
Cuts at: 0 64 1353
Size: 64 1289
NsiI A_TGCA'T
Cuts at: 0 591 1353
Size: 591 762
NspI r_CATG'y
Cuts at: 0 1252 1353
Size: 1252 101
PciI A'CATG_T
Cuts at: 0 1248 1353
Size: 1248 105
PshAI GACnn'nnGTC
Cuts at: 0 46 1353
Size: 46 1307
PsiI TTA'TAA
Cuts at: 0 611 1196 1353
Size: 611 585 157
Fragments arranged by size:
611 585 157
PspOMI G'GGCC_C
Cuts at: 0 17 1353
Size: 17 1336
SacI G_AGCT'C
Cuts at: 0 855 866 1353
Size: 855 11 487
Fragments arranged by size:
855 487 11
SfoI GGC'GCC
Cuts at: 0 11 1353
Size: 11 1342
SmlI C'TyrA_G
Cuts at: 0 1094 1276 1353
Size: 1094 182 77
Fragments arranged by size:
1094 182 77
SspI AAT'ATT
Cuts at: 0 790 1353
Size: 790 563
XbaI T'CTAG_A
Cuts at: 0 405 1353
Size: 405 948
XmnI GAAnn'nnTTC
Cuts at: 0 749 827 1168 1353
Size: 749 78 341 185
Fragments arranged by size:
749 341 185 78
Enzymes that do cut:
AcuI AflIII AgeI AlfI AlwNI ApaI ApoI BanI
BanII BbeI BbsI BfrBI BglII Bme1580I BmtI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrFI BstAPI BstYI BtgI
DraI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII HincII KasI MmeI MslI
NarI NheI NotI NsiI NspI PciI PshAI PsiI
PspOMI SacI SfoI SmlI SspI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BglI BlpI
BmgBI BmrI BplI BsaAI BsaBI BsaXI BseYI BsgI
BsiWI BsmBI BspEI BspMI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII EciI EcoRI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI
NcoI NdeI NgoMIV NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI