################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BciVI BfrI BglII BlnI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcsI 383 GAATTT [GA]^AATT[CT] AflIII 1196 ACATGT A^C[GA][CT]GT AhdI 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 67 GTGCAC G^TGCAC AlwI 459 GATCC GGATC(4/5) AlwNI 550 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1165 CCTGAGG CC^T[ACGT]AGG ApaLI 67 GTGCAC G^TGCAC ApoI 383 GAATTT [GA]^AATT[CT] AspEI 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 583 CACGTG CAC^GTG BbsI 265 GTCTTC GAAGAC(2/6) BbvCI 123 CCTCAGC CCTCAGC(-5/-2) BcgI 706 GCAGACAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 860 TGATCA T^GATCA BfaI 1276 CTAG C^TAG BlpI 144 GCTCAGC GC^T[ACGT]AGC Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC BpuAI 265 GTCTTC GAAGAC(2/6) BspLU11I 1196 ACATGT A^CATGT BspMI 1160 ACCTGC ACCTGC(4/8) BsrBI 212 GAGCGG CCGCTC(-3/-3) BsrFI 800 ACCGGC [GA]^CCGG[CT] BsrI 424 ACTGG ACTGG(1/-1) BssHII 137 GCGCGC G^CGCGC Bsu36I 1165 CCTGAGG CC^T[ACGT]AGG CelII 144 GCTCAGC GC^T[ACGT]AGC Cfr10I 800 ACCGGC [GA]^CCGG[CT] EagI 194 CGGCCG C^GGCCG Eam1105I 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 194 CGGCCG C^GGCCG Eco52I 194 CGGCCG C^GGCCG Eco81I 1165 CCTGAGG CC^T[ACGT]AGG EcoNI 1161 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 816 CCAAGG C^C[AT][AT]GG FbaI 860 TGATCA T^GATCA MaeI 1276 CTAG C^TAG MaeIII 40 GTGAC ^GT[ACGT]AC MseI 968 TTAA T^TAA MslI 605 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PmaCI 583 CACGTG CAC^GTG PmlI 583 CACGTG CAC^GTG PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT] PstI 117 CTGCAG CTGCA^G PvuII 1036 CAGCTG CAG^CTG SacI 940 GAGCTC GAGCT^C ScaI 734 AGTACT AGT^ACT SfcI 117 CTGCAG C^T[GA][CT]AG SspI 405 AATATT AAT^ATT StuI 1256 AGGCCT AGG^CCT StyI 816 CCAAGG C^C[AT][AT]GG Tru9I 968 TTAA T^TAA Tsp45I 40 GTGAC ^GT[GC]AC Tsp509I 384 AATT ^AATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 526 GGGCCC GGGCC^C 975 GGGCCC Asp700I 501 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1266 GAATGACTTC AspHI 67 GTGCAC G[AT]GC[AT]^C 940 GAGCTC BsaAI 583 CACGTG [CT]AC^GT[GA] 1216 TACGTG BsaI 103 GGTCTC GGTCTC(1/5) 578 GGTCTC BsaWI 656 ACCGGA [AT]^CCGG[AT] 1293 ACCGGA BsgI 438 GTGCAG GTGCAG(16/14) 704 GTGCAG BsiEI 194 CGGCCG CG[GA][CT]^CG 787 CGGTCG BsiHKAI 67 GTGCAC G[AT]GC[AT]^C 940 GAGCTC BsrDI 519 CATTGC GCAATG(2/0) 1065 CATTGC KspI 84 CCGCGG CCGC^GG 244 CCGCGG NspI 317 ACATGC [GA]CATG^[CT] 1196 ACATGT SacII 84 CCGCGG CCGC^GG 244 CCGCGG SmaI 635 CCCGGG CCC^GGG 1205 CCCGGG TaqI 413 TCGA T^CGA 686 TCGA TthHB8I 413 TCGA T^CGA 686 TCGA XcmI 926 CCAAGCCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1211 CCAAGTACGTGATGG XmaCI 635 CCCGGG C^CCGGG 1205 CCCGGG XmaI 635 CCCGGG C^CCGGG 1205 CCCGGG XmnI 501 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1266 GAATGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TfiI TseI TspRI