Restriction map for
pFN21AE0590
(Circular) (Six-base) MAPSORT of: pFN21AE0590.seq Check: 3280 from: 1 to: 6010
pFN21AE0590 6010 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 2728 4073 5457 5725 1632
Size: 865 231 1345 1384 268 1917
Fragments arranged by size:
1917 1384 1345 865 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 3201 4909 5886 1203
Size: 1998 1708 977 1327
Fragments arranged by size:
1998 1708 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 3022 4739 3022
Size: 1717 4293
AleI CACnn'nnGTG
Cuts at: 1166 2614 1166
Size: 1448 4562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 1184
Size: 933 5077
AlwNI CAG_nnn'CTG
Cuts at: 2560 3753 5325 2560
Size: 1193 1572 3245
Fragments arranged by size:
3245 1572 1193
ApaI G_GGCC'C
Cuts at: 2535 2984 2535
Size: 449 5561
ApaLI G'TGCA_C
Cuts at: 2072 4067 5223 2072
Size: 1995 1156 2859
Fragments arranged by size:
2859 1995 1156
ApoI r'AATT_y
Cuts at: 1493 2388 3319 3545 3697 5806 1493
Size: 895 931 226 152 2109 1697
Fragments arranged by size:
2109 1697 931 895 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2640 3210 3335 1870
Size: 73 300 397 570 125 4545
Fragments arranged by size:
4545 570 397 300 125 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2178 2256 2925 3100
Size: 325 217 627 297 94 78 669 175
Cuts at: 3100 3331 4786 618
Size: 231 1455 1842
Fragments arranged by size:
1842 1455 669 627 325 297 231 217
175 94 78
BanII G_rGCy'C
Cuts at: 1391 2244 2535 2949 2984 3306 3329 1391
Size: 853 291 414 35 322 23 4072
Fragments arranged by size:
4072 853 414 322 291 35 23
BbeI G_GCGC'C
Cuts at: 2088 2182 2260 2088
Size: 94 78 5838
Fragments arranged by size:
5838 94 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 928
Size: 971 364 4675
Fragments arranged by size:
4675 971 364
BbvCI CC'TCA_GC
Cuts at: 2129 2129
Size: 6010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2700 2734 4201 4235 1903
Size: 34 763 34 1467 34 3678
Fragments arranged by size:
3678 1467 763 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 2865 1548
Size: 1317 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2231 2254
Size: 107 122 71 631 605 490 69 23
Cuts at: 2254 4621 136
Size: 2367 1525
Fragments arranged by size:
2367 1525 631 605 490 122 107 71
69 23
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 2150 3427 2150
Size: 1277 4733
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2535 2984 4071 5227 1790
Size: 286 459 449 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 459 449 286
BmgBI CAC'GTC
Cuts at: 1318 2071 3232 1318
Size: 753 1161 4096
Fragments arranged by size:
4096 1161 753
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2439 3741 4699
Size: 416 53 83 399 24 1022 1302 958
Cuts at: 4699 442
Size: 1753
Fragments arranged by size:
1753 1302 1022 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2282 2507 2582 2597 2963 4670 1239
Size: 800 243 225 75 15 366 1707 2579
Fragments arranged by size:
2579 1707 800 366 243 225 75 15
Bpu10I CC'TnA_GC
Cuts at: 2129 2129
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 2589 4673 882
Size: 617 615 475 2084 2219
Fragments arranged by size:
2219 2084 617 615 475
BsaAI yAC'GTr
Cuts at: 493 1204 2590 3223 3801 493
Size: 711 1386 633 578 2702
Fragments arranged by size:
2702 1386 711 633 578
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2179 2257 2421
Size: 53 83 186 936 552 94 78 164
Cuts at: 2421 2595 3817 4199 4834 275
Size: 174 1222 382 635 1451
Fragments arranged by size:
1451 1222 936 635 552 382 186 174
164 94 83 78 53
BsaWI w'CCGG_w
Cuts at: 2661 3298 4443 4841 5115 5262 5673 2661
Size: 637 1145 398 274 147 411 2998
Fragments arranged by size:
2998 1145 637 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2075 2738 3107 3110 3251 1186
Size: 850 39 663 369 3 141 3945
Fragments arranged by size:
3945 850 663 369 141 39 3
BseYI C'CCAG_C
Cuts at: 2080 2104 5213 5709 2080
Size: 24 3109 496 2381
Fragments arranged by size:
3109 2381 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2464 2730 1163
Size: 573 728 266 4443
Fragments arranged by size:
4443 728 573 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2202 2795 3460 4221 5249 1540
Size: 275 186 201 593 665 761 1028 2301
Fragments arranged by size:
2301 1028 761 665 593 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2949 4071 4156 5227 2076
Size: 873 1122 85 1071 2859
Fragments arranged by size:
2859 1122 1071 873 85
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2244 2535 2949 2984 3306 3329
Size: 399 286 168 291 414 35 322 23
Cuts at: 3329 4071 4156 5227 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 322 291 286
168 85 35 23
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3174 3365 844
Size: 2330 191 3489
Fragments arranged by size:
3489 2330 191
BsrBI CCG'CTC
Cuts at: 1394 1823 2219 3897 1394
Size: 429 396 1678 3507
Fragments arranged by size:
3507 1678 429 396
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 3069 4505 4679 65
Size: 1183 1275 546 1436 174 1396
Fragments arranged by size:
1436 1396 1275 1183 546 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2805 4654 4841 1700
Size: 110 995 1849 187 2869
Fragments arranged by size:
2869 1849 995 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 2142 1925
Size: 217 5793
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 3340 3707 4092 4109 5561 5647 5659 6005
Size: 1368 367 385 17 1452 86 12 346
Cuts at: 6005 1972
Size: 1977
Fragments arranged by size:
1977 1452 1368 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3171 3171
Size: 6010
BtgI C'CryG_G
Cuts at: 513 1065 2089 2249 2327 513
Size: 552 1024 160 78 4196
Fragments arranged by size:
4196 1024 552 160 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 3526 4320 4340 2306
Size: 1220 794 20 3976
Fragments arranged by size:
3976 1220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2199 3033
Size: 54 1425 50 275 18 333 36 834
Cuts at: 3033 3457 4346 5975 8
Size: 424 889 1629 43
Fragments arranged by size:
1629 1425 889 834 424 333 275 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2199 3457 1537
Size: 275 387 1258 4090
Fragments arranged by size:
4090 1258 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3940 1353
Size: 2587 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2252 2610 4597 4971 5117 387
Size: 1064 138 663 358 1987 374 146 1280
Fragments arranged by size:
1987 1280 1064 663 374 358 146 138
EcoICRI GAG'CTC
Cuts at: 2947 2947
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2282 2497 2507 2582 2597 2728
Size: 393 407 243 215 10 75 15 131
Cuts at: 2728 2963 4073 4670 5457 5725 1239
Size: 235 1110 597 787 268 1524
Fragments arranged by size:
1524 1110 787 597 407 393 268 243
235 215 131 75 15 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3170 3170
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2531 2532 2644 2980 2981 2531
Size: 1 112 336 1 5560
Fragments arranged by size:
5560 336 112 1 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 2088 2136 2157 2182 2260 2339 5157 1678
Size: 410 48 21 25 78 79 2818 2531
Fragments arranged by size:
2818 2531 410 79 78 48 25 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 3008 3040 4651
Size: 32 135 32 488 32 916 32 1611
Cuts at: 4651 4683 4725 4757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1611 916 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KasI G'GCGC_C
Cuts at: 2084 2178 2256 2084
Size: 94 78 5838
Fragments arranged by size:
5838 94 78
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 2700 2976 2982 5124 5308 1305
Size: 1113 282 276 6 2142 184 2007
Fragments arranged by size:
2142 2007 1113 282 276 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2614 3968 4327 4486 518
Size: 648 1448 1354 359 159 2042
Fragments arranged by size:
2042 1448 1354 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2251 2296 3043 3426 4103 5251 5496
Size: 485 160 45 747 383 677 1148 245
Cuts at: 5496 1606
Size: 2120
Fragments arranged by size:
2120 1148 747 677 485 383 245 160
45
NarI GG'CG_CC
Cuts at: 2085 2179 2257 2085
Size: 94 78 5838
Fragments arranged by size:
5838 94 78
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5458
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2326 3205 3368 4913 1132
Size: 97 1097 879 163 1545 2229
Fragments arranged by size:
2229 1545 1097 879 163 97
PasI CC'CwG_GG
Cuts at: 1348 2496 1348
Size: 1148 4862
PciI A'CATG_T
Cuts at: 3201 4909 3201
Size: 1708 4302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6010
PfoI T'CCnGG_A
Cuts at: 2016 3195 2016
Size: 1179 4831
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PmlI CAC'GTG
Cuts at: 2590 2590
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 2644
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2531 2980 2531
Size: 449 5561
PstI C_TGCA'G
Cuts at: 830 2126 3362 830
Size: 1296 1236 3478
Fragments arranged by size:
3478 1296 1236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 3043 3043
Size: 6010
SacI G_AGCT'C
Cuts at: 2949 2949
Size: 6010
SacII CC_GC'GG
Cuts at: 2092 2252 2092
Size: 160 5850
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 2741 4258 5970 2741
Size: 1517 1712 2781
Fragments arranged by size:
2781 1712 1517
SfcI C'TryA_G
Cuts at: 826 1045 2122 3358 4493 5174 5365 826
Size: 219 1077 1236 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 1077 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2180 2258 2086
Size: 94 78 5838
Fragments arranged by size:
5838 94 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 2642 3212 3337 1872
Size: 770 570 125 4545
Fragments arranged by size:
4545 770 570 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SrfI GCCC'GGGC
Cuts at: 3212 3212
Size: 6010
SspI AAT'ATT
Cuts at: 5 52 2412 3934 5
Size: 47 2360 1522 2081
Fragments arranged by size:
2360 2081 1522 47
StuI AGG'CCT
Cuts at: 1517 3263 1517
Size: 1746 4264
StyI C'CwwG_G
Cuts at: 513 1065 2821 3449 513
Size: 552 1756 628 3074
Fragments arranged by size:
3074 1756 628 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2518 4037 4054 4207 4392 4837 325
Size: 1255 938 1519 17 153 185 445 1498
Fragments arranged by size:
1519 1498 1255 938 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 3078 4256
Size: 274 80 33 51 1443 762 339 1178
Cuts at: 4256 5968 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1178 762 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2397 2429 5970 6002 2312
Size: 32 53 32 3541 32 2320
Fragments arranged by size:
3541 2320 53 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2938 3223 2938
Size: 285 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 2640 3210 3335 1870
Size: 770 570 125 4545
Fragments arranged by size:
4545 770 570 125
XmnI GAAnn'nnTTC
Cuts at: 2510 3275 4139 2510
Size: 765 864 4381
Fragments arranged by size:
4381 864 765
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SwaI