##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BalI        BamHI       BanII       BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BseRI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        Ksp632I     
MamI        MboI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NcoI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspV        
PacI        PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        Sau3AI      ScaI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp509I     Van91I      XbaI        XcmI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccI          487  GTCTAC               GT^[AC][GT]AC
AfaI          175  GTAC                 GT^AC
AlwNI         613  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          643  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         656  TGCGCA               TGC^GCA
BbvCI         495  CCTCAGC              CCTCAGC(-5/-2)
BfaI           25  CTAG                 C^TAG
BsrBI         249  GAGCGG               CCGCTC(-3/-3)
BstAPI        540  GCACATCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI          496  CTCAG                C^T[ACGT]AG
FspI          656  TGCGCA               TGC^GCA
MaeI           25  CTAG                 C^TAG
MaeIII        128  GTGAC                ^GT[ACGT]AC
NspI          659  GCATGT               [GA]CATG^[CT]
PaeR7I        170  CTCGAG               C^TCGAG
PflFI         643  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          283  CTGCAG               CTGCA^G
RsaI          175  GTAC                 GT^AC
SbfI          282  CCTGCAGG             CCTGCA^GG
SexAI         731  ACCTGGT              A^CC[AT]GGT
SfaNI         621  GATGC                GCATC(5/9)
SmlI          170  CTCGAG               C^T[CT][GA]AG
Sse8387I      282  CCTGCAGG             CCTGCA^GG
Tsp45I        128  GTGAC                ^GT[GC]AC
Tth111I       643  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          170  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Alw44I         79  GTGCAC               G^TGCAC
              508  GTGCAC              
ApaLI          79  GTGCAC               G^TGCAC
              508  GTGCAC              
BsaI          529  GAGACC               GGTCTC(1/5)
              704  GAGACC              
BsmAI         529  GAGAC                GTCTC(1/5)
              704  GAGAC               
BspMI         536  ACCTGC               ACCTGC(4/8)
              309  GCAGGT              
EagI          566  CGGCCG               C^GGCCG
              743  CGGCCG              
EclXI         566  CGGCCG               C^GGCCG
              743  CGGCCG              
Eco52I        566  CGGCCG               C^GGCCG
              743  CGGCCG              
KspI          455  CCGCGG               CCGC^GG
              563  CCGCGG              
MaeII         339  ACGT                 A^CGT
              506  ACGT                
PpuMI         134  GGGACCT              [GA]G^G[AT]CC[CT]
              149  GGGACCT             
SacII         455  CCGCGG               CCGC^GG
              563  CCGCGG              
SfcI          185  CTATAG               C^T[GA][CT]AG
              283  CTGCAG              
SfiI          280  GGCCTGCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              289  GGCCGCAGAGGCC       
TspRI          29  CACTG                CA[GC]TG[ACGT][ACGT]^
               82  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaI        AvaII       BanI        BbeI        BbvI        
BcnI        BglI        BmyI        BsaHI       BsaJI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        KasI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NgoMIV      NlaIII      
NlaIV       Sau96I      ScrFI       SfoI        SmaI        
TaqI        TseI        TthHB8I     XmaCI       XmaI