################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 1174 GTAGAC GT^[AC][GT]AC AccII 1348 CGCG CG^CG AciI 1347 CCGC CCGC(-3/-1) AclI 1429 AACGTT AA^CGTT AcyI 1108 GGCGCC G[GA]^CG[CT]C AflIII 1285 ACATGT A^C[GA][CT]GT AlwNI 1544 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1594 GGGCCC GGGCC^C AspHI 326 GAGCTC G[AT]GC[AT]^C AvaII 142 GGACC G^G[AT]CC BalI 536 TGGCCA TGG^CCA BanI 1108 GGCGCC G^G[CT][GA]CC BbeI 1108 GGCGCC GGCGC^C BbsI 965 GAAGAC GAAGAC(2/6) BbvCI 1324 GCTGAGG CCTCAGC(-5/-2) BpuAI 965 GAAGAC GAAGAC(2/6) BsaHI 1108 GGCGCC G[GA]^CG[CT]C BsiHKAI 326 GAGCTC G[AT]GC[AT]^C BspHI 417 TCATGA T^CATGA BspLU11I 1285 ACATGT A^CATGT BstUI 1348 CGCG CG^CG BstYI 957 GGATCT [GA]^GATC[CT] EcoT14I 17 CCAAGG C^C[AT][AT]GG FauI 1346 CCCGC CCCGC(4/6) Hin1I 1108 GGCGCC G[GA]^CG[CT]C HphI 685 GGTGA GGTGA(8/7) KasI 1108 GGCGCC G^GCGCC MflI 957 GGATCT [GA]^GATC[CT] MluNI 536 TGGCCA TGG^CCA MscI 536 TGGCCA TGG^CCA MspA1I 1586 CAGCTG C[AC]G^C[GT]G MvnI 1348 CGCG CG^CG NarI 1108 GGCGCC GG^CGCC PflMI 1585 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1429 AACGTT AA^CGTT PvuII 1586 CAGCTG CAG^CTG RcaI 417 TCATGA T^CATGA SacI 326 GAGCTC GAGCT^C ScaI 1125 AGTACT AGT^ACT SfoI 1108 GGCGCC GGC^GCC SmlI 43 CTCAAG C^T[CT][GA]AG SphI 556 GCATGC GCATG^C SspI 1511 AATATT AAT^ATT StyI 17 CCAAGG C^C[AT][AT]GG Van91I 1585 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1491 TCTAGA T^CTAGA XhoII 957 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 617 AAATTC [GA]^AATT[CT] 798 GAATTT AlwI 957 GGATC GGATC(4/5) 1563 GGATC ApoI 617 AAATTC [GA]^AATT[CT] 798 GAATTT BcnI 25 CCGGG CC^[GC]GG 1228 CCCGG BpmI 323 CTGGAG CTGGAG(16/14) 1367 CTGGAG BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG 1503 CCTGGG BsmAI 1282 GAGAC GTCTC(1/5) 478 GTCTC BsmFI 1032 GGGAC GGGAC(10/14) 1456 GTCCC EaeI 536 TGGCCA [CT]^GGCC[GA] 582 CGGCCA HaeII 3 GGCGCT [GA]GCGC^[CT] 1108 GGCGCC HindIII 101 AAGCTT A^AGCTT 541 AAGCTT HinfI 423 GATTC G^A[ACGT]TC 577 GATTC MslI 586 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 930 CATGTTAGTG NciI 25 CCGGG CC^[GC]GG 1228 CCCGG PstI 405 CTGCAG CTGCA^G 1541 CTGCAG SapI 901 GCTCTTC GCTCTTC(1/4) 994 GCTCTTC TaqI 462 TCGA T^CGA 1131 TCGA TfiI 423 GATTC G^A[AT]TC 577 GATTC TthHB8I 462 TCGA T^CGA 1131 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AfaI AluI Asp700I BanII BbvI BfaI BmyI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XmnI