(Circular) (Six-base) MAPSORT of: pFN21AA0002.seq Check: 3047 from: 1 to: 6349 pFN21AA0002 6349 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3692 1937 Size: 1243 512 4594 Fragments arranged by size: 4594 1243 512 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 3435 4476 4849 1563 Size: 1872 1041 373 3063 Fragments arranged by size: 3063 1872 1041 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 2634 2811 3477 4412 5796 6064 1632 Size: 738 264 177 666 935 1384 268 1917 Fragments arranged by size: 1917 1384 935 738 666 268 264 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 3290 5248 6225 1203 Size: 2087 1958 977 1327 Fragments arranged by size: 2087 1958 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2265 1184 Size: 1081 5268 AlwNI CAG_nnn'CTG Cuts at: 3554 4092 5664 3554 Size: 538 1572 4239 Fragments arranged by size: 4239 1572 538 ApaI G_GGCC'C Cuts at: 3603 3603 Size: 6349 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2622 2803 3658 3884 4036 6145 1493 Size: 1129 181 855 226 152 2109 1697 Fragments arranged by size: 2109 1697 1129 855 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 3674 1870 Size: 73 1731 4545 Fragments arranged by size: 4545 1731 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3066 3099 3066 Size: 33 6316 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 3670 5125 618 Size: 325 217 627 1326 557 1455 1842 Fragments arranged by size: 1842 1455 1326 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 2335 2633 3603 3668 1391 Size: 944 298 970 65 4072 Fragments arranged by size: 4072 970 944 298 65 BbeI G_GCGC'C Cuts at: 3117 3117 Size: 6349 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2977 928 Size: 971 1078 4300 Fragments arranged by size: 4300 1078 971 BbvCI CC'TCA_GC Cuts at: 3330 3330 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 3603 4410 5566 1790 Size: 1813 807 1156 2573 Fragments arranged by size: 2573 1813 1156 807 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3637 4080 5038 Size: 416 53 83 399 24 2220 443 958 Cuts at: 5038 442 Size: 1753 Fragments arranged by size: 2220 1753 958 443 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2349 3393 5009 1239 Size: 1110 1044 1616 2579 Fragments arranged by size: 2579 1616 1110 1044 Bpu10I CC'TnA_GC Cuts at: 2556 3330 2556 Size: 774 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 4474 5339 5637 2033 Size: 2441 865 298 2745 Fragments arranged by size: 2745 2441 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5012 882 Size: 617 3513 2219 Fragments arranged by size: 3513 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4140 493 Size: 711 2936 2702 Fragments arranged by size: 2936 2702 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3114 4156 4538 5173 Size: 53 83 186 936 1581 1042 382 635 Cuts at: 5173 275 Size: 1451 Fragments arranged by size: 1581 1451 1042 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 1178 Size: 30 165 30 774 30 5320 Fragments arranged by size: 5320 774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6349 BseYI C'CCAG_C Cuts at: 5552 6048 5552 Size: 496 5853 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5776 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2335 4410 4495 5566 2335 Size: 2075 85 1071 3118 Fragments arranged by size: 3118 2075 1071 85 BsmI GAATG_Cn' Cuts at: 3860 3953 3860 Size: 93 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2335 2633 3603 3668 4410 4495 5566 Size: 399 545 298 970 65 742 85 1071 Cuts at: 5566 1391 Size: 2174 Fragments arranged by size: 2174 1071 970 742 545 399 298 85 65 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 2422 4238 5968 2422 Size: 1816 1730 2803 Fragments arranged by size: 2803 1816 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3704 844 Size: 2860 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4844 5018 65 Size: 1183 3596 174 1396 Fragments arranged by size: 3596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2962 3679 4046 4431 4448 5900 5986 5998 Size: 990 717 367 385 17 1452 86 12 Cuts at: 5998 6344 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 990 717 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2730 3865 4659 4679 2730 Size: 1135 794 20 4400 Fragments arranged by size: 4400 1135 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 2587 3796 Size: 54 1425 50 275 18 711 46 1209 Cuts at: 3796 4685 6314 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1209 889 711 275 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2653 2913 3006 4279 1353 Size: 723 577 260 93 1273 3423 Fragments arranged by size: 3423 1273 723 577 260 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2333 2333 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2370 2634 2811 3393 3477 4412 Size: 393 717 21 264 177 582 84 935 Cuts at: 4412 5009 5796 6064 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 935 787 717 597 582 393 268 264 177 84 21 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 2012 3117 5496 1678 Size: 334 1105 2379 2531 Fragments arranged by size: 2531 2379 1105 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2684 2716 4990 Size: 32 135 32 896 32 184 32 2274 Cuts at: 4990 5022 5064 5096 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2274 896 184 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 2106 2546 748 Size: 1358 440 4551 Fragments arranged by size: 4551 1358 440 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KasI G'GCGC_C Cuts at: 3113 3113 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 3049 3418 5463 5647 1305 Size: 970 774 369 2045 184 2007 Fragments arranged by size: 2045 2007 970 774 369 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2543 10 Size: 54 1425 343 711 3816 Fragments arranged by size: 3816 1425 711 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2595 2939 4307 4666 4825 518 Size: 648 1429 344 1368 359 159 2042 Fragments arranged by size: 2042 1429 1368 648 359 344 159 MspA1I CmG'CkG Cuts at: 1606 3593 3765 4442 5590 5835 1606 Size: 1987 172 677 1148 245 2120 Fragments arranged by size: 2120 1987 1148 677 245 172 NarI GG'CG_CC Cuts at: 3114 3114 Size: 6349 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 NdeI CA'TA_TG Cuts at: 387 2143 2277 3280 387 Size: 1756 134 1003 3456 Fragments arranged by size: 3456 1756 1003 134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2565 3294 3481 3707 5252 1132 Size: 97 1336 729 187 226 1545 2229 Fragments arranged by size: 2229 1545 1336 729 226 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 3290 5248 3290 Size: 1958 4391 PflMI CCAn_nnn'nTGG Cuts at: 1108 3596 1108 Size: 2488 3861 PfoI T'CCnGG_A Cuts at: 2337 3295 2337 Size: 958 5391 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 3599 3599 Size: 6349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2220 2252 2220 Size: 32 6317 PstI C_TGCA'G Cuts at: 830 2414 3550 3701 830 Size: 1584 1136 151 3478 Fragments arranged by size: 3478 1584 1136 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 3593 3593 Size: 6349 SacI G_AGCT'C Cuts at: 2335 2335 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SapI GCTCTTCn'nnn_ Cuts at: 2913 3006 2913 Size: 93 6256 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 3132 4597 6309 3132 Size: 1465 1712 3172 Fragments arranged by size: 3172 1712 1465 SfcI C'TryA_G Cuts at: 826 1045 2410 2994 3144 3546 3697 4832 5513 Size: 219 1365 584 150 402 151 1135 681 Cuts at: 5513 5704 826 Size: 191 1471 Fragments arranged by size: 1471 1365 1135 681 584 402 219 191 151 150 SfoI GGC'GCC Cuts at: 3115 3115 Size: 6349 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 4453 5354 5616 820 Size: 197 926 105 2405 901 262 1553 Fragments arranged by size: 2405 1553 926 901 262 197 105 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 2565 3707 1132 Size: 1433 1142 3774 Fragments arranged by size: 3774 1433 1142 SspI AAT'ATT Cuts at: 5 52 3518 4273 5 Size: 47 3466 755 2081 Fragments arranged by size: 3466 2081 755 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 3788 513 Size: 552 939 18 1766 3074 Fragments arranged by size: 3074 1766 939 552 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4376 4393 4546 4731 5176 325 Size: 1255 2796 17 153 185 445 1498 Fragments arranged by size: 2796 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2236 2727 3130 Size: 274 80 33 51 1443 259 491 403 Cuts at: 3130 3412 3556 4595 6307 96 Size: 282 144 1039 1712 138 Fragments arranged by size: 1712 1443 1039 491 403 282 274 259 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 6309 6341 3006 Size: 32 3271 32 3014 Fragments arranged by size: 3271 3014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3496 3685 3496 Size: 189 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2355 2535 3345 4478 2355 Size: 180 810 1133 4226 Fragments arranged by size: 4226 1133 810 180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI