################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 360 GTCGAC GT^[AC][GT]AC AfaI 565 GTAC GT^AC AvaII 456 GGACC G^G[AT]CC AviII 413 TGCGCA TGC^GCA BamHI 321 GGATCC G^GATCC BbrPI 466 CACGTG CAC^GTG BglI 307 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 130 CTCCAG CTGGAG(16/14) BsaAI 466 CACGTG [CT]AC^GT[GA] BsaWI 387 TCCGGT [AT]^CCGG[AT] BsmAI 210 GTCTC GTCTC(1/5) BsmFI 527 GGGAC GGGAC(10/14) BsmI 514 GAATGC GAATGC(1/-1) BsrBI 265 GAGCGG CCGCTC(-3/-3) BsrDI 195 CATTGC GCAATG(2/0) BstAPI 416 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 54 GGTGACC G^GT[ACGT]ACC BstPI 54 GGTGACC G^GT[ACGT]ACC DraII 314 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 80 CCATGG C^C[GA][CT]GG EcoO109I 314 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 54 GGTGACC G^GT[ACGT]ACC EcoT22I 516 ATGCAT ATGCA^T FspI 413 TGCGCA TGC^GCA HaeII 491 AGCGCC [GA]GCGC^[CT] NcoI 80 CCATGG C^CATGG NsiI 516 ATGCAT ATGCA^T NspI 278 GCATGC [GA]CATG^[CT] PaeR7I 297 CTCGAG C^TCGAG PmaCI 466 CACGTG CAC^GTG PmlI 466 CACGTG CAC^GTG PstI 448 CTGCAG CTGCA^G RsaI 565 GTAC GT^AC SacI 177 GAGCTC GAGCT^C SalI 360 GTCGAC G^TCGAC SapI 63 GAAGAGC GCTCTTC(1/4) SfcI 448 CTGCAG C^T[GA][CT]AG SmlI 297 CTCGAG C^T[CT][GA]AG SphI 278 GCATGC GCATG^C XhoI 297 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 177 GAGCTC G[AT]GC[AT]^C 733 GAGCAC AvaI 140 CCCGAG C^[CT]CG[GA]G 297 CTCGAG BanI 354 GGCACC G^G[CT][GA]CC 390 GGTGCC BanII 177 GAGCTC G[GA]GC[CT]^C 841 GAGCCC BsiHKAI 177 GAGCTC G[AT]GC[AT]^C 733 GAGCAC BsoBI 140 CCCGAG C^[CT]CG[GA]G 297 CTCGAG BstYI 321 GGATCC [GA]^GATC[CT] 698 AGATCC EarI 13 GAAGAG CTCTTC(1/4) 63 GAAGAG EcoT14I 80 CCATGG C^C[AT][AT]GG 716 CCAAGG HincII 157 GTCAAC GT[CT]^[GA]AC 360 GTCGAC HindII 157 GTCAAC GT[CT]^[GA]AC 360 GTCGAC Ksp632I 13 GAAGAG CTCTTC(1/4) 63 GAAGAG MaeIII 55 GTGAC ^GT[ACGT]AC 397 GTCAC MflI 321 GGATCC [GA]^GATC[CT] 698 AGATCC MslI 540 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 813 CACACAGGTG MspA1I 440 CCGCTG C[AC]G^C[GT]G 882 CCGCTG StyI 80 CCATGG C^C[AT][AT]GG 716 CCAAGG Tsp45I 55 GTGAC ^GT[GC]AC 397 GTCAC XhoII 321 GGATCC [GA]^GATC[CT] 698 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AlwI BalI BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MluNI MnlI MscI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I