################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeIII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwI 271 GGATC GGATC(4/5) AlwNI 475 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 142 AGCGCT AGC^GCT Asp700I 289 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 411 GGATAC GTATCC(6/5) BglII 200 AGATCT A^GATCT BsmAI 20 GAGAC GTCTC(1/5) BsmBI 20 GAGACG CGTCTC(1/5) BstF5I 392 GGATG GGATG(2/0) BstYI 200 AGATCT [GA]^GATC[CT] Cac8I 45 GCGAGC GC[ACGT]^[ACGT]GC CfoI 143 GCGC GCG^C DraII 63 AGGACCT [GA]G^G[ACGT]CC[CT] Eco47III 142 AGCGCT AGC^GCT EcoO109I 63 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 16 GCGGG CCCGC(4/6) FokI 392 GGATG GGATG(9/13) HaeII 142 AGCGCT [GA]GCGC^[CT] HapII 59 CCGG C^CGG HhaI 143 GCGC GCG^C HinP1I 143 GCGC G^CGC HpaII 59 CCGG C^CGG MflI 200 AGATCT [GA]^GATC[CT] MseI 338 TTAA T^TAA MspI 59 CCGG C^CGG NlaIV 52 GGAGCC GG[ACGT]^[ACGT]CC PpuMI 63 AGGACCT [GA]G^G[AT]CC[CT] SfaNI 247 GCATC GCATC(5/9) SspI 400 AATATT AAT^ATT Tru9I 338 TTAA T^TAA XhoII 200 AGATCT [GA]^GATC[CT] XmnI 289 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AvaII 64 GGACC G^G[AT]CC 430 GGACC BpmI 374 CTCCAG CTGGAG(16/14) 50 CTGGAG BsrI 113 CCAGT ACTGG(1/-1) 134 CCAGT Cfr13I 64 GGACC G^G[ACGT]CC 430 GGACC DdeI 98 CTAAG C^T[ACGT]AG 146 CTGAG MspA1I 158 CAGCTG C[AC]G^C[GT]G 425 CAGCTG MwoI 49 GCTGGAGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 480 GCTGTGGGAGC NlaIII 457 CATG CATG^ 520 CATG PvuII 158 CAGCTG CAG^CTG 425 CAGCTG Sau96I 64 GGACC G^G[ACGT]CC 430 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI AluI BbvI DpnII Fnu4HI ItaI MboI MboII MnlI NdeII Sau3AI TseI