################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstUI BstXI CfoI Cfr10I CpoI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MluI MluNI MroI MscI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 1379 GGTACC G^GTACC AciI 937 GCGG CCGC(-3/-1) AclI 930 AACGTT AA^CGTT AhdI 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 763 GATCC GGATC(4/5) AlwNI 1102 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 426 ATTAAT AT^TAAT AsnI 426 ATTAAT AT^TAAT Asp718I 1379 GGTACC G^GTACC AspEI 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2437 GAGCTC G[AT]GC[AT]^C AvaI 2023 CTCGAG C^[CT]CG[GA]G BanI 1379 GGTACC G^G[CT][GA]CC BbsI 255 GAAGAC GAAGAC(2/6) BglII 1785 AGATCT A^GATCT BlpI 1059 GCTAAGC GC^T[ACGT]AGC BpmI 1190 CTCCAG CTGGAG(16/14) Bpu1102I 1059 GCTAAGC GC^T[ACGT]AGC BpuAI 255 GAAGAC GAAGAC(2/6) BsaBI 1341 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1310 CTGCAC GTGCAG(16/14) BsiHKAI 2437 GAGCTC G[AT]GC[AT]^C BsmFI 2048 GGGAC GGGAC(10/14) BsoBI 2023 CTCGAG C^[CT]CG[GA]G BspDI 1541 ATCGAT AT^CGAT BspMI 324 GCAGGT ACCTGC(4/8) Bst1107I 2039 GTATAC GTA^TAC BstAPI 1155 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 381 GGTTACC G^GT[ACGT]ACC BstPI 381 GGTTACC G^GT[ACGT]ACC BstZ17I 2039 GTATAC GTA^TAC Cac8I 2301 GCAAGC GC[ACGT]^[ACGT]GC CelII 1059 GCTAAGC GC^T[ACGT]AGC ClaI 1541 ATCGAT AT^CGAT DraI 969 TTTAAA TTT^AAA DraII 2048 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1383 CCATGG C^C[GA][CT]GG Eam1105I 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 2048 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 381 GGTTACC G^GT[ACGT]ACC EcoRV 601 GATATC GAT^ATC EcoT22I 125 ATGCAT ATGCA^T HgaI 1999 GACGC GACGC(5/10) HindIII 467 AAGCTT A^AGCTT KpnI 1379 GGTACC GGTAC^C MamI 1341 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1280 CAATTG C^AATTG MspA1I 1105 CAGCTG C[AC]G^C[GT]G MunI 1280 CAATTG C^AATTG NcoI 1383 CCATGG C^CATGG NdeI 535 CATATG CA^TATG NsiI 125 ATGCAT ATGCA^T PaeR7I 2023 CTCGAG C^TCGAG PpuMI 2048 GGGACCT [GA]G^G[AT]CC[CT] PshBI 426 ATTAAT AT^TAAT Psp1406I 930 AACGTT AA^CGTT PvuII 1105 CAGCTG CAG^CTG SacI 2437 GAGCTC GAGCT^C SapI 1319 GCTCTTC GCTCTTC(1/4) ScaI 169 AGTACT AGT^ACT SexAI 1464 ACCTGGT A^CC[AT]GGT SfcI 417 CTATAG C^T[GA][CT]AG SspI 1855 AATATT AAT^ATT XcmI 386 CCAAAACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2023 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1897 GTCTAC GT^[AC][GT]AC 2039 GTATAC AocI 1651 CCTAAGG CC^T[ACGT]AGG 1792 CCTCAGG AvaII 283 GGACC G^G[AT]CC 2049 GGACC BanII 1317 GGGCTC G[GA]GC[CT]^C 2437 GAGCTC BciVI 2326 GGATAC GTATCC(6/5) 199 GTATCC BclI 374 TGATCA T^GATCA 1845 TGATCA BfaI 767 CTAG C^TAG 1150 CTAG BsaI 2254 GAGACC GGTCTC(1/5) 1298 GGTCTC BsaWI 2184 ACCGGA [AT]^CCGG[AT] 2330 ACCGGA BseRI 979 GAGGAG GAGGAG(10/8) 1503 GAGGAG BspHI 1677 TCATGA T^CATGA 2375 TCATGA BspLU11I 1535 ACATGT A^CATGT 1774 ACATGT BstYI 762 AGATCC [GA]^GATC[CT] 1785 AGATCT Bsu36I 1651 CCTAAGG CC^T[ACGT]AGG 1792 CCTCAGG EarI 1320 CTCTTC CTCTTC(1/4) 1002 GAAGAG Eco81I 1651 CCTAAGG CC^T[ACGT]AGG 1792 CCTCAGG EcoRI 24 GAATTC G^AATTC 441 GAATTC EcoT14I 500 CCAAGG C^C[AT][AT]GG 1383 CCATGG FbaI 374 TGATCA T^GATCA 1845 TGATCA HaeIII 96 GGCC GG^CC 498 GGCC HapII 2185 CCGG C^CGG 2331 CCGG HpaII 2185 CCGG C^CGG 2331 CCGG Ksp632I 1320 CTCTTC CTCTTC(1/4) 1002 GAAGAG MaeI 767 CTAG C^TAG 1150 CTAG MflI 762 AGATCC [GA]^GATC[CT] 1785 AGATCT MspI 2185 CCGG C^CGG 2331 CCGG NlaIV 1379 GGTACC GG[ACGT]^[ACGT]CC 2048 GGGACC PflMI 203 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2168 CCATTAAATGG PleI 625 GACTC GAGTC(4/5) 1452 GAGTC RcaI 1677 TCATGA T^CATGA 2375 TCATGA StyI 500 CCAAGG C^C[AT][AT]GG 1383 CCATGG Van91I 203 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2168 CCATTAAATGG XhoII 762 AGATCC [GA]^GATC[CT] 1785 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AfaI AflIII AluI ApoI Asp700I BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI