(Circular) (Six-base) MAPSORT of: pFN21AB7308.seq Check: 441 from: 1 to: 7171 pFN21AB7308 7171 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2320 2320 Size: 7171 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3903 4045 4514 1937 Size: 1966 142 469 4594 Fragments arranged by size: 4594 1966 469 142 Acc65I G'GTAC_C Cuts at: 3384 4492 3384 Size: 1108 6063 AclI AA'CG_TT Cuts at: 1563 2936 5298 5671 1563 Size: 1373 2362 373 3063 Fragments arranged by size: 3063 2362 1373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2858 3144 3312 4249 4418 5234 6618 Size: 413 813 286 168 937 169 816 1384 Cuts at: 6618 6886 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 937 816 813 413 286 268 169 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6974 AflIII A'CryG_T Cuts at: 1203 3540 3779 4230 6070 7047 1203 Size: 2337 239 451 1840 977 1327 Fragments arranged by size: 2337 1840 1327 977 451 239 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 3899 5900 3899 Size: 2001 5170 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4087 1184 Size: 2903 4268 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4201 4233 4201 Size: 32 7139 AlwNI CAG_nnn'CTG Cuts at: 3112 4914 6486 3112 Size: 1802 1572 3797 Fragments arranged by size: 3797 1802 1572 ApaLI G'TGCA_C Cuts at: 5228 6384 5228 Size: 1156 6015 ApoI r'AATT_y Cuts at: 1493 2029 2093 2446 3263 3721 3815 3834 3992 Size: 536 64 353 817 458 94 19 158 Cuts at: 3992 4480 4706 4858 6967 1493 Size: 488 226 152 2109 1697 Fragments arranged by size: 2109 1697 817 536 488 458 353 226 158 152 94 64 19 AseI AT'TA_AT Cuts at: 160 1032 2432 5725 160 Size: 872 1400 3293 1606 Fragments arranged by size: 3293 1606 1400 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4028 4496 1870 Size: 73 2085 468 4545 Fragments arranged by size: 4545 2085 468 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3376 3409 3376 Size: 33 7138 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3384 4492 5947 618 Size: 325 217 627 1597 1108 1455 1842 Fragments arranged by size: 1842 1597 1455 1108 627 325 217 BanII G_rGCy'C Cuts at: 1391 3326 4446 4490 1391 Size: 1935 1120 44 4072 Fragments arranged by size: 4072 1935 1120 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 928 Size: 971 368 5832 Fragments arranged by size: 5832 971 368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5362 5396 1903 Size: 34 3425 34 3678 Fragments arranged by size: 3678 3425 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2215 4325 5063 6273 2215 Size: 2110 738 1210 3113 Fragments arranged by size: 3113 2110 1210 738 BclI T'GATC_A Cuts at: 1548 2379 3850 1548 Size: 831 1471 4869 Fragments arranged by size: 4869 1471 831 BfrBI ATG'CAT Cuts at: 2132 2132 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5782 136 Size: 107 122 71 631 605 4110 1525 Fragments arranged by size: 4110 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3790 7166 3790 Size: 3376 3795 BlpI GC'TnA_GC Cuts at: 3065 4588 3065 Size: 1523 5648 Bme1580I G_kGCm'C Cuts at: 1790 2581 3560 3746 5232 6388 1790 Size: 791 979 186 1486 1156 2573 Fragments arranged by size: 2573 1486 1156 979 791 186 BmgBI CAC'GTC Cuts at: 1318 4231 1318 Size: 2913 4258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 3329 3570 Size: 416 53 83 399 24 654 1258 241 Cuts at: 3570 3719 4902 5860 442 Size: 149 1183 958 1753 Fragments arranged by size: 1753 1258 1183 958 654 416 399 241 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3180 5831 1239 Size: 1941 2651 2579 Fragments arranged by size: 2651 2579 1941 Bpu10I CC'TnA_GC Cuts at: 4270 4270 Size: 7171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3399 3824 5296 6161 6459 3399 Size: 425 1472 865 298 4111 Fragments arranged by size: 4111 1472 865 425 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3309 4253 5834 882 Size: 617 1810 944 1581 2219 Fragments arranged by size: 2219 1810 1581 944 617 BsaAI yAC'GTr Cuts at: 493 1204 4962 493 Size: 711 3758 2702 Fragments arranged by size: 3758 2702 711 BsaBI GATnn'nnATC Cuts at: 3350 7165 3350 Size: 3815 3356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4978 5360 5995 275 Size: 53 83 186 936 3445 382 635 1451 Fragments arranged by size: 3445 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4189 4335 5604 6002 6276 6423 6834 4189 Size: 146 1269 398 274 147 411 4526 Fragments arranged by size: 4526 1269 411 398 274 147 146 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4201 4231 1178 Size: 30 165 30 2798 30 4118 Fragments arranged by size: 4118 2798 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2999 3523 1186 Size: 1813 524 4834 Fragments arranged by size: 4834 1813 524 BseYI C'CCAG_C Cuts at: 4350 6374 6870 4350 Size: 2024 496 4651 Fragments arranged by size: 4651 2024 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3300 1163 Size: 573 1564 5034 Fragments arranged by size: 5034 1564 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4621 5382 6410 1540 Size: 275 186 2620 761 1028 2301 Fragments arranged by size: 2620 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4446 5232 5317 6388 4446 Size: 786 85 1071 5229 Fragments arranged by size: 5229 1071 786 85 BsmI GAATG_Cn' Cuts at: 4682 4775 4682 Size: 93 7078 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2581 3326 3560 3746 4446 4490 5232 Size: 399 791 745 234 186 700 44 742 Cuts at: 5232 5317 6388 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 791 745 742 700 399 234 186 85 44 BspEI T'CCGG_A Cuts at: 6834 6834 Size: 7171 BspHI T'CATG_A Cuts at: 3682 4380 5060 6790 3682 Size: 698 680 1730 4063 Fragments arranged by size: 4063 1730 698 680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 4526 844 Size: 1476 2206 3489 Fragments arranged by size: 3489 2206 1476 BsrBI CCG'CTC Cuts at: 1394 1823 5058 1394 Size: 429 3235 3507 Fragments arranged by size: 3507 3235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5666 5840 65 Size: 1183 4418 174 1396 Fragments arranged by size: 4418 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5815 6002 1700 Size: 110 4005 187 2869 Fragments arranged by size: 4005 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 3166 794 Size: 2372 4799 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 2386 1184 Size: 1202 5969 BstYI r'GATC_y Cuts at: 1972 2767 3790 4501 4868 5253 5270 6722 6808 Size: 795 1023 711 367 385 17 1452 86 Cuts at: 6808 6820 7166 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1023 795 711 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4046 4046 Size: 7171 Bsu36I CC'TnA_GG Cuts at: 3657 3798 3657 Size: 141 7030 BtgI C'CryG_G Cuts at: 513 1065 2004 3388 513 Size: 552 939 1384 4296 Fragments arranged by size: 4296 1384 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 2049 4687 5481 5501 2049 Size: 2638 794 20 3719 Fragments arranged by size: 3719 2638 794 20 ClaI AT'CG_AT Cuts at: 1554 3547 1554 Size: 1993 5178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 2976 4475 2976 Size: 1499 5672 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6178 809 Size: 507 4862 1802 Fragments arranged by size: 4862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4618 5507 7136 Size: 54 1425 50 275 18 2788 889 1629 Cuts at: 7136 8 Size: 43 Fragments arranged by size: 2788 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3002 3331 5101 1353 Size: 1649 329 1770 3423 Fragments arranged by size: 3423 1770 1649 329 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5758 6132 6278 387 Size: 1064 138 4169 374 146 1280 Fragments arranged by size: 4169 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4444 4444 Size: 7171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2858 3144 3180 3312 4249 4418 Size: 393 413 813 286 36 132 937 169 Cuts at: 4418 5234 5831 6618 6886 1239 Size: 816 597 787 268 1524 Fragments arranged by size: 1524 937 816 813 787 597 413 393 286 268 169 132 36 EcoO109I rG'GnC_Cy Cuts at: 4054 4054 Size: 7171 EcoRI G'AATT_C Cuts at: 2029 2446 4480 4858 2029 Size: 417 2034 378 4342 Fragments arranged by size: 4342 2034 417 378 EcoRV GAT'ATC Cuts at: 2608 2608 Size: 7171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3809 3809 Size: 7171 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 6318 1678 Size: 4640 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 2689 2721 3029 Size: 32 135 32 695 32 390 32 308 Cuts at: 3029 3061 3209 3241 3242 3274 3332 3364 3504 Size: 32 148 32 1 32 58 32 140 Cuts at: 3504 3536 3664 3696 3885 3917 4330 4362 5812 Size: 32 128 32 189 32 413 32 1450 Cuts at: 5812 5844 5886 5918 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1450 695 413 390 308 189 148 140 135 128 58 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 4515 4761 669 Size: 988 281 2577 246 3079 Fragments arranged by size: 3079 2577 988 281 246 HindIII A'AGCT_T Cuts at: 748 2472 748 Size: 1724 5447 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 3388 4496 3388 Size: 1108 6063 MfeI C'AATT_G Cuts at: 3285 4770 3285 Size: 1485 5686 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6285 6469 1305 Size: 4980 184 2007 Fragments arranged by size: 4980 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5349 Fragments arranged by size: 5349 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3687 3911 5129 5488 5647 518 Size: 648 1143 1378 224 1218 359 159 2042 Fragments arranged by size: 2042 1378 1218 1143 648 359 224 159 MspA1I CmG'CkG Cuts at: 1606 3112 4587 5264 6412 6657 1606 Size: 1506 1475 677 1148 245 2120 Fragments arranged by size: 2120 1506 1475 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3388 513 Size: 552 939 1384 4296 Fragments arranged by size: 4296 1384 939 552 NdeI CA'TA_TG Cuts at: 387 2541 387 Size: 2154 5017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NsiI A_TGCA'T Cuts at: 2134 2134 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2270 3544 3783 4529 6074 1132 Size: 97 1041 1274 239 746 1545 2229 Fragments arranged by size: 2229 1545 1274 1041 746 239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7171 PciI A'CATG_T Cuts at: 3540 3779 6070 3540 Size: 239 2291 4641 Fragments arranged by size: 4641 2291 239 PflMI CCAn_nnn'nTGG Cuts at: 1108 2214 4179 1108 Size: 1106 1965 4100 Fragments arranged by size: 4100 1965 1106 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3960 3992 4201 4233 5240 5272 6753 6785 3960 Size: 32 209 32 1007 32 1481 32 4346 Fragments arranged by size: 4346 1481 1007 209 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4054 4054 Size: 7171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4401 4741 4401 Size: 340 6831 PstI C_TGCA'G Cuts at: 830 4523 830 Size: 3693 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 3112 3112 Size: 7171 SacI G_AGCT'C Cuts at: 4446 4446 Size: 7171 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SapI GCTCTTCn'nnn_ Cuts at: 3331 3331 Size: 7171 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 2176 5419 7131 2176 Size: 3243 1712 2216 Fragments arranged by size: 3243 2216 1712 SexAI A'CCwGG_T Cuts at: 3469 3469 Size: 7171 SfcI C'TryA_G Cuts at: 826 1045 2422 4519 5654 6335 6526 826 Size: 219 1377 2097 1135 681 191 1471 Fragments arranged by size: 2097 1471 1377 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4498 1872 Size: 2626 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3414 3839 4028 5275 6176 6438 Size: 197 926 1471 425 189 1247 901 262 Cuts at: 6438 820 Size: 1553 Fragments arranged by size: 1553 1471 1247 926 901 425 262 197 189 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7171 SphI G_CATG'C Cuts at: 1132 4529 1132 Size: 3397 3774 SspI AAT'ATT Cuts at: 5 52 3862 5095 5 Size: 47 3810 1233 2081 Fragments arranged by size: 3810 2081 1233 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7171 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 3388 4610 513 Size: 552 939 501 883 1222 3074 Fragments arranged by size: 3074 1222 939 883 552 501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5198 5215 5368 5553 5998 325 Size: 1255 3618 17 153 185 445 1498 Fragments arranged by size: 3618 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2174 2774 5417 Size: 274 80 33 51 1443 197 600 2643 Cuts at: 5417 7129 96 Size: 1712 138 Fragments arranged by size: 2643 1712 1443 600 274 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3457 3489 7131 7163 3457 Size: 32 3642 32 3465 Fragments arranged by size: 3642 3465 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7171 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2398 Size: 7171 XhoI C'TCGA_G Cuts at: 1943 4028 1943 Size: 2085 5086 XmaI C'CCGG_G Cuts at: 1870 4496 1870 Size: 2626 4545 XmnI GAAnn'nnTTC Cuts at: 2871 3273 3792 5300 2871 Size: 402 519 1508 4742 Fragments arranged by size: 4742 1508 519 402 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI