(Circular) (Six-base) MAPSORT of: pFN21AB5954.seq Check: 6084 from: 1 to: 6349 pFN21AB5954 6349 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3118 3118 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3390 3509 3692 1937 Size: 1453 119 183 4594 Fragments arranged by size: 4594 1453 183 119 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 4412 5796 6064 1632 Size: 824 1956 1384 268 1917 Fragments arranged by size: 1956 1917 1384 824 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 2727 3393 3533 3609 5248 6225 1203 Size: 1524 666 140 76 1639 977 1327 Fragments arranged by size: 1639 1524 1327 977 666 140 76 AgeI A'CCGG_T Cuts at: 3060 5180 3060 Size: 2120 4229 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 3014 1166 Size: 1848 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6349 AlwNI CAG_nnn'CTG Cuts at: 4092 5664 4092 Size: 1572 4777 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2431 3245 3658 3884 4036 6145 1493 Size: 938 814 413 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 814 413 226 152 AseI AT'TA_AT Cuts at: 160 1032 3297 4903 160 Size: 872 2265 1606 1606 Fragments arranged by size: 2265 1606 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3674 1870 Size: 73 298 1433 4545 Fragments arranged by size: 4545 1433 298 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2846 2994 3670 5125 618 Size: 325 217 627 1059 148 676 1455 1842 Fragments arranged by size: 1842 1455 1059 676 627 325 217 148 BanII G_rGCy'C Cuts at: 1391 3135 3668 1391 Size: 1744 533 4072 Fragments arranged by size: 4072 1744 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3594 3628 4540 4574 1903 Size: 34 1657 34 912 34 3678 Fragments arranged by size: 3678 1657 912 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 2661 1548 Size: 1113 5236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 4410 5566 1790 Size: 2620 1156 2573 Fragments arranged by size: 2620 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3394 3610 1318 Size: 2076 216 4057 Fragments arranged by size: 4057 2076 216 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2015 1056 Size: 959 5390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2571 2664 5009 1239 Size: 1332 93 2345 2579 Fragments arranged by size: 2579 2345 1332 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4474 5339 5637 4474 Size: 865 298 5186 Fragments arranged by size: 5186 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 5012 882 Size: 617 1413 2100 2219 Fragments arranged by size: 2219 2100 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 2140 3016 4140 493 Size: 711 936 876 1124 2702 Fragments arranged by size: 2702 1124 936 876 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3060 4782 5180 5454 5601 6012 3060 Size: 1722 398 274 147 411 3397 Fragments arranged by size: 3397 1722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3323 3353 1178 Size: 30 165 30 1920 30 4174 Fragments arranged by size: 4174 1920 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2661 2684 2733 2802 1186 Size: 1475 23 49 69 4733 Fragments arranged by size: 4733 1475 69 49 23 BseYI C'CCAG_C Cuts at: 2400 2929 5552 6048 2400 Size: 529 2623 496 2701 Fragments arranged by size: 2701 2623 529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5776 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3135 4410 4495 5566 3135 Size: 1275 85 1071 3918 Fragments arranged by size: 3918 1275 1071 85 BsmI GAATG_Cn' Cuts at: 2559 3860 3953 2559 Size: 1301 93 4955 Fragments arranged by size: 4955 1301 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2032 3600 2032 Size: 1568 4781 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3135 3668 4410 4495 5566 1391 Size: 399 1345 533 742 85 1071 2174 Fragments arranged by size: 2174 1345 1071 742 533 399 85 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 3118 3455 3704 844 Size: 2086 188 337 249 3489 Fragments arranged by size: 3489 2086 337 249 188 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4844 5018 65 Size: 1183 3596 174 1396 Fragments arranged by size: 3596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3060 4993 5180 1700 Size: 110 1250 1933 187 2869 Fragments arranged by size: 2869 1933 1250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 2758 1184 Size: 1574 4775 BstYI r'GATC_y Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344 Size: 1707 367 385 17 1452 86 12 346 Cuts at: 6344 1972 Size: 1977 Fragments arranged by size: 1977 1707 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3510 3510 Size: 6349 Bsu36I CC'TnA_GG Cuts at: 3363 3363 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2526 2754 3541 513 Size: 552 939 48 474 228 787 3321 Fragments arranged by size: 3321 939 787 552 474 228 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2061 3370 487 Size: 683 56 835 1309 3466 Fragments arranged by size: 3466 1309 835 683 56 BtsI GCAGTG_nn' Cuts at: 2542 2550 3865 4659 4679 2542 Size: 8 1315 794 20 4212 Fragments arranged by size: 4212 1315 794 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 2083 3653 2083 Size: 1570 4779 DraIII CAC_nnn'GTG Cuts at: 2766 2766 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2684 5356 809 Size: 507 1368 2672 1802 Fragments arranged by size: 2672 1802 1368 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2593 3796 4685 Size: 54 1425 50 275 18 763 1203 889 Cuts at: 4685 6314 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1203 889 763 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 2269 2861 2874 4279 1353 Size: 872 44 592 13 1405 3423 Fragments arranged by size: 3423 1405 872 592 44 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2501 4936 5310 5456 387 Size: 1064 138 912 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 912 374 146 138 EcoICRI GAG'CTC Cuts at: 3133 3133 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2571 2664 4412 5009 5796 6064 Size: 393 824 115 93 1748 597 787 268 Cuts at: 6064 1239 Size: 1524 Fragments arranged by size: 1748 1524 824 787 597 393 268 115 93 EcoNI CCTnn'n_nnAGG Cuts at: 2643 2643 Size: 6349 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 EcoRV GAT'ATC Cuts at: 3259 3259 Size: 6349 FspI TGC'GCA Cuts at: 2417 4855 2417 Size: 2438 3911 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2742 2774 3243 3275 3323 Size: 32 135 32 1170 32 469 32 48 Cuts at: 3323 3355 4990 5022 5064 5096 1373 Size: 32 1635 32 42 32 2626 Fragments arranged by size: 2626 1635 1170 469 135 48 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3391 3693 3939 669 Size: 988 281 1453 302 246 3079 Fragments arranged by size: 3079 1453 988 302 281 246 HindIII A'AGCT_T Cuts at: 748 2861 748 Size: 2113 4236 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2840 3045 5463 5647 1305 Size: 1535 205 2418 184 2007 Fragments arranged by size: 2418 2007 1535 205 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2595 10 Size: 54 1425 343 763 3764 Fragments arranged by size: 3764 1425 763 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3014 4307 4666 4825 518 Size: 648 1848 1293 359 159 2042 Fragments arranged by size: 2042 1848 1293 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3765 4442 5590 5835 1606 Size: 2159 677 1148 245 2120 Fragments arranged by size: 2159 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2754 3541 513 Size: 552 939 750 787 3321 Fragments arranged by size: 3321 939 787 750 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NheI G'CTAG_C Cuts at: 1052 2011 1052 Size: 959 5390 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2731 3352 3537 3707 5252 1132 Size: 97 1502 621 185 170 1545 2229 Fragments arranged by size: 2229 1545 1502 621 185 170 97 PasI CC'CwG_GG Cuts at: 1348 2935 1348 Size: 1587 4762 PciI A'CATG_T Cuts at: 2727 3533 5248 2727 Size: 806 1715 3828 Fragments arranged by size: 3828 1715 806 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 3016 3016 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3471 3919 3471 Size: 448 5901 PstI C_TGCA'G Cuts at: 830 3115 3701 830 Size: 2285 586 3478 Fragments arranged by size: 3478 2285 586 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 SacI G_AGCT'C Cuts at: 3135 3135 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3389 3691 1936 Size: 1453 302 4594 Fragments arranged by size: 4594 1453 302 SbfI CC_TGCA'GG Cuts at: 3115 3701 3115 Size: 586 5763 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SexAI A'CCwGG_T Cuts at: 2762 2762 Size: 6349 SfcI C'TryA_G Cuts at: 826 1045 3111 3697 4832 5513 5704 826 Size: 219 2066 586 1135 681 191 1471 Fragments arranged by size: 2066 1471 1135 681 586 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 2243 3676 1872 Size: 371 1433 4545 Fragments arranged by size: 4545 1433 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 4453 5354 5616 820 Size: 197 926 2510 901 262 1553 Fragments arranged by size: 2510 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2140 4140 493 Size: 1647 2000 2702 Fragments arranged by size: 2702 2000 1647 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 3099 4273 5 Size: 47 3047 1174 2081 Fragments arranged by size: 3047 2081 1174 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2754 3541 3788 513 Size: 552 939 63 687 787 247 3074 Fragments arranged by size: 3074 939 787 687 552 247 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 4376 4393 4546 4731 5176 325 Size: 1255 469 2327 17 153 185 445 1498 Fragments arranged by size: 2327 1498 1255 469 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2910 3006 4595 Size: 274 80 33 51 1443 933 96 1589 Cuts at: 4595 6307 96 Size: 1712 138 Fragments arranged by size: 1712 1589 1443 933 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 3011 3043 6309 6341 2480 Size: 32 499 32 3266 32 2488 Fragments arranged by size: 3266 2488 499 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3548 3548 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 2241 3674 1870 Size: 371 1433 4545 Fragments arranged by size: 4545 1433 371 XmnI GAAnn'nnTTC Cuts at: 2124 4478 2124 Size: 2354 3995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI