(Circular) (Six-base) MAPSORT of: pFN21AB7787.seq Check: 2869 from: 1 to: 6079 pFN21AB7787 6079 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3306 3422 1937 Size: 1369 116 4594 Fragments arranged by size: 4594 1369 116 Acc65I G'GTAC_C Cuts at: 2166 3400 2166 Size: 1234 4845 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2349 2384 2497 3085 4142 5526 5794 1632 Size: 717 35 113 588 1057 1384 268 1917 Fragments arranged by size: 1917 1384 1057 717 588 268 113 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2399 4978 5955 1203 Size: 1196 2579 977 1327 Fragments arranged by size: 2579 1327 1196 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 2302 4808 2302 Size: 2506 3573 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 6079 ApaLI G'TGCA_C Cuts at: 2066 4136 5292 2066 Size: 2070 1156 2853 Fragments arranged by size: 2853 2070 1156 ApoI r'AATT_y Cuts at: 1493 2140 2322 3051 3135 3356 3388 3614 3766 Size: 647 182 729 84 221 32 226 152 Cuts at: 3766 5875 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 729 647 226 221 182 152 84 32 AseI AT'TA_AT Cuts at: 160 1032 2312 4633 160 Size: 872 1280 2321 1606 Fragments arranged by size: 2321 1606 1280 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2622 3042 3400 4855 Size: 325 217 627 379 456 420 358 1455 Cuts at: 4855 618 Size: 1842 Fragments arranged by size: 1842 1455 627 456 420 379 358 325 217 BanII G_rGCy'C Cuts at: 1391 2116 3398 1391 Size: 725 1282 4072 Fragments arranged by size: 4072 1282 725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3144 928 Size: 971 1245 3863 Fragments arranged by size: 3863 1245 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 2700 1548 Size: 1152 4927 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 4690 136 Size: 107 122 71 631 605 439 2579 1525 Fragments arranged by size: 2579 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2070 2116 4140 5296 1790 Size: 280 46 2024 1156 2573 Fragments arranged by size: 2573 2024 1156 280 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2165 2565 2817 Size: 416 53 83 399 24 748 400 252 Cuts at: 2817 3385 3810 4768 442 Size: 568 425 958 1753 Fragments arranged by size: 1753 958 748 568 425 416 400 399 252 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2391 2391 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2661 2913 3249 3333 4739 1239 Size: 1422 252 336 84 1406 2579 Fragments arranged by size: 2579 1422 1406 336 252 84 Bpu10I CC'TnA_GC Cuts at: 3108 3108 Size: 6079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3317 4204 5069 5367 3317 Size: 887 865 298 4029 Fragments arranged by size: 4029 887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 2641 4742 882 Size: 617 780 362 2101 2219 Fragments arranged by size: 2219 2101 780 617 362 BsaAI yAC'GTr Cuts at: 493 1204 2402 3870 493 Size: 711 1198 1468 2702 Fragments arranged by size: 2702 1468 1198 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2678 3077 1186 Size: 1324 168 399 4188 Fragments arranged by size: 4188 1324 399 168 BseYI C'CCAG_C Cuts at: 2248 3282 5282 5778 2248 Size: 1034 2000 496 2549 Fragments arranged by size: 2549 2000 1034 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2169 1163 Size: 573 433 5073 Fragments arranged by size: 5073 573 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 3109 4140 4225 5296 2070 Size: 1039 1031 85 1071 2853 Fragments arranged by size: 2853 1071 1039 1031 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2116 3109 3398 4140 4225 5296 Size: 399 280 46 993 289 742 85 1071 Cuts at: 5296 1391 Size: 2174 Fragments arranged by size: 2174 1071 993 742 399 289 280 85 46 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2191 3434 844 Size: 1347 1243 3489 Fragments arranged by size: 3489 1347 1243 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2744 96 Size: 2648 3431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 2044 3290 1184 Size: 860 1246 3973 Fragments arranged by size: 3973 1246 860 BstYI r'GATC_y Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074 Size: 1437 367 385 17 1452 86 12 346 Cuts at: 6074 1972 Size: 1977 Fragments arranged by size: 1977 1452 1437 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3526 4415 6044 Size: 54 1425 50 275 18 1696 889 1629 Cuts at: 6044 8 Size: 43 Fragments arranged by size: 1696 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 4009 1353 Size: 1007 1649 3423 Fragments arranged by size: 3423 1649 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2384 2497 2661 2913 3085 3249 Size: 393 717 35 113 164 252 172 164 Cuts at: 3249 3333 4142 4739 5526 5794 1239 Size: 84 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 717 597 393 268 252 172 164 164 113 84 35 EcoNI CCTnn'n_nnAGG Cuts at: 2473 2473 Size: 6079 EcoO109I rG'GnC_Cy Cuts at: 2112 2113 2224 2112 Size: 1 111 5967 Fragments arranged by size: 5967 111 1 EcoRI G'AATT_C Cuts at: 3051 3135 3388 3766 3051 Size: 84 253 378 5364 Fragments arranged by size: 5364 378 253 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2499 2499 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2638 2670 2692 Size: 32 135 32 716 32 318 32 22 Cuts at: 2692 2724 2932 2964 4720 4752 4794 4826 1373 Size: 32 208 32 1756 32 42 32 2626 Fragments arranged by size: 2626 1756 716 318 208 135 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3669 669 Size: 988 281 1485 246 3079 Fragments arranged by size: 3079 1485 988 281 246 HindIII A'AGCT_T Cuts at: 748 2844 2929 748 Size: 2096 85 3898 Fragments arranged by size: 3898 2096 85 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 2170 3404 2170 Size: 1234 4845 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 5193 5377 1305 Size: 810 3078 184 2007 Fragments arranged by size: 3078 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4257 Fragments arranged by size: 4257 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4037 4396 4555 518 Size: 648 2871 359 159 2042 Fragments arranged by size: 2871 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 3495 4172 5320 5565 1606 Size: 756 1133 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 756 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 2489 387 Size: 2102 3977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3305 3437 4982 1132 Size: 97 2076 132 1545 2229 Fragments arranged by size: 2229 2076 1545 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 1108 Size: 933 5146 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3336 1613 Size: 1723 4356 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 6079 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2362 2362 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 3427 4562 5243 5434 826 Size: 219 2382 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3296 4183 5084 5346 820 Size: 197 926 1353 887 901 262 1553 Fragments arranged by size: 1553 1353 926 901 887 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 3266 1517 Size: 1749 4330 StyI C'CwwG_G Cuts at: 513 1065 2004 2446 3518 513 Size: 552 939 442 1072 3074 Fragments arranged by size: 3074 1072 939 552 442 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2744 4325 6037 Size: 274 80 33 51 1443 767 1581 1712 Cuts at: 6037 96 Size: 138 Fragments arranged by size: 1712 1581 1443 767 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6039 6071 6039 Size: 32 6047 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 2483 2510 2762 2941 4208 2483 Size: 27 252 179 1267 4354 Fragments arranged by size: 4354 1267 252 179 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI