(Circular) (Six-base) MAPSORT of: pFN21AB7750.seq Check: 7114 from: 1 to: 6109 pFN21AB7750 6109 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2243 2344 4172 5556 5824 1632 Size: 461 150 101 1828 1384 268 1917 Fragments arranged by size: 1917 1828 1384 461 268 150 101 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 2641 2643 5008 5985 1203 Size: 1438 2 2365 977 1327 Fragments arranged by size: 2365 1438 1327 977 2 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2234 1184 Size: 1050 5059 AlwNI CAG_nnn'CTG Cuts at: 2272 3852 5424 2272 Size: 1580 1572 2957 Fragments arranged by size: 2957 1580 1572 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 3275 3434 1870 Size: 73 1332 159 4545 Fragments arranged by size: 4545 1332 159 73 BamHI G'GATC_C Cuts at: 3333 3439 3806 3333 Size: 106 367 5636 Fragments arranged by size: 5636 367 106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4885 618 Size: 325 217 627 1643 1455 1842 Fragments arranged by size: 1842 1643 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2740 3107 3153 3428 1391 Size: 1349 367 46 275 4072 Fragments arranged by size: 4072 1349 367 275 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 928 Size: 971 222 4916 Fragments arranged by size: 4916 971 222 BbvCI CC'TCA_GC Cuts at: 3019 3019 Size: 6109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3063 4720 136 Size: 107 122 71 631 605 1391 1657 1525 Fragments arranged by size: 1657 1525 1391 631 605 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 4170 5326 1790 Size: 2380 1156 2573 Fragments arranged by size: 2573 2380 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 2481 2906 Size: 416 53 83 399 24 602 462 425 Cuts at: 2906 3840 4798 442 Size: 934 958 1753 Fragments arranged by size: 1753 958 934 602 462 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 2759 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2739 4769 1239 Size: 805 695 2030 2579 Fragments arranged by size: 2579 2030 805 695 Bpu10I CC'TnA_GC Cuts at: 2044 2786 3019 2044 Size: 742 233 5134 Fragments arranged by size: 5134 742 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4234 5099 5397 4234 Size: 865 298 4946 Fragments arranged by size: 4946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2919 4772 882 Size: 617 1420 1853 2219 Fragments arranged by size: 2219 1853 1420 617 BsaAI yAC'GTr Cuts at: 493 1204 2644 3900 493 Size: 711 1440 1256 2702 Fragments arranged by size: 2702 1440 1256 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3090 3336 4542 4940 5214 5361 5772 3090 Size: 246 1206 398 274 147 411 3427 Fragments arranged by size: 3427 1206 411 398 274 246 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2533 2563 2766 2796 2987 Size: 30 165 30 1130 30 203 30 191 Cuts at: 2987 3017 1178 Size: 30 4270 Fragments arranged by size: 4270 1130 203 191 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2554 2995 2998 3001 3004 3007 3305 1186 Size: 1368 441 3 3 3 3 298 3990 Fragments arranged by size: 3990 1368 441 298 3 3 3 3 BseYI C'CCAG_C Cuts at: 3107 3186 3230 5312 5808 3107 Size: 79 44 2082 496 3408 Fragments arranged by size: 3408 2082 496 79 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 3305 1163 Size: 573 450 1119 3967 Fragments arranged by size: 3967 1119 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2816 3559 4320 5348 1540 Size: 275 186 815 743 761 1028 2301 Fragments arranged by size: 2301 1028 815 761 743 275 186 BsiHKAI G_wGCw'C Cuts at: 2981 4170 4255 5326 2981 Size: 1189 85 1071 3764 Fragments arranged by size: 3764 1189 1071 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 BsmBI CGTCTCn'nnnn_ Cuts at: 3250 3250 Size: 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2740 2981 3107 3153 3428 4170 4255 Size: 399 950 241 126 46 275 742 85 Cuts at: 4255 5326 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 950 742 399 275 241 126 85 46 BspEI T'CCGG_A Cuts at: 3090 5772 3090 Size: 2682 3427 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3464 844 Size: 2620 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 2204 3098 4169 5181 2204 Size: 894 1071 1012 3132 Fragments arranged by size: 3132 1071 1012 894 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2703 2703 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2433 3333 3439 3806 4191 4208 5660 5746 Size: 461 900 106 367 385 17 1452 86 Cuts at: 5746 5758 6104 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 900 461 385 367 346 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2246 2670 2246 Size: 424 5685 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 4456 Fragments arranged by size: 4456 939 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3297 487 Size: 683 56 2071 3299 Fragments arranged by size: 3299 2071 683 56 BtsI GCAGTG_nn' Cuts at: 2959 3239 3625 4419 4439 2959 Size: 280 386 794 20 4629 Fragments arranged by size: 4629 794 386 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2705 2813 Size: 54 1425 50 275 18 549 326 108 Cuts at: 2813 2847 3556 4445 6074 8 Size: 34 709 889 1629 43 Fragments arranged by size: 1629 1425 889 709 549 326 275 108 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2813 3556 1537 Size: 275 1001 743 4090 Fragments arranged by size: 4090 1001 743 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2172 2219 3114 4039 1353 Size: 798 21 47 895 925 3423 Fragments arranged by size: 3423 925 895 798 47 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2093 2243 2344 2739 4172 4769 Size: 393 412 49 150 101 395 1433 597 Cuts at: 4769 5556 5824 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1433 787 597 412 395 393 268 150 101 49 EcoO109I rG'GnC_Cy Cuts at: 2104 2269 2782 3226 3312 2104 Size: 165 513 444 86 4901 Fragments arranged by size: 4901 513 444 165 86 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 EcoRV GAT'ATC Cuts at: 2422 2422 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 2407 3202 5256 1678 Size: 729 795 2054 2531 Fragments arranged by size: 2531 2054 795 729 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2611 2643 2766 2798 2987 Size: 32 135 32 1039 32 123 32 189 Cuts at: 2987 3019 4750 4782 4824 4856 1373 Size: 32 1731 32 42 32 2626 Fragments arranged by size: 2626 1731 1039 189 135 123 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2420 2991 5223 5407 1305 Size: 1115 571 2232 184 2007 Fragments arranged by size: 2232 2007 1115 571 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 3738 Fragments arranged by size: 3738 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 2837 4067 4426 4585 518 Size: 648 1209 462 1230 359 159 2042 Fragments arranged by size: 2042 1230 1209 648 462 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2364 2818 2846 2871 2922 3174 3525 Size: 485 273 454 28 25 51 252 351 Cuts at: 3525 4202 5350 5595 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 485 454 351 273 252 245 51 28 25 NcoI C'CATG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 4456 Fragments arranged by size: 4456 939 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 2813 3556 2813 Size: 743 5366 NspI r_CATG'y Cuts at: 1132 1229 2314 3467 5012 1132 Size: 97 1085 1153 1545 2229 Fragments arranged by size: 2229 1545 1153 1085 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 2903 1108 Size: 1164 631 4314 Fragments arranged by size: 4314 1164 631 PfoI T'CCnGG_A Cuts at: 2265 2755 2265 Size: 490 5619 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PmlI CAC'GTG Cuts at: 2644 2644 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2782 3226 3312 2269 Size: 513 444 86 5066 Fragments arranged by size: 5066 513 444 86 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PstI C_TGCA'G Cuts at: 830 3461 830 Size: 2631 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2364 2871 2364 Size: 507 5602 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SanDI GG'GwC_CC Cuts at: 3312 3312 Size: 6109 SapI GCTCTTCn'nnn_ Cuts at: 2172 2219 2172 Size: 47 6062 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2947 3457 4592 5273 5464 826 Size: 219 1902 510 1135 681 191 1471 Fragments arranged by size: 1902 1471 1135 681 510 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4213 5114 5376 820 Size: 197 926 2270 901 262 1553 Fragments arranged by size: 2270 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6109 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2166 2931 3048 3153 3213 Size: 552 939 96 66 765 117 105 60 Cuts at: 3213 3548 513 Size: 335 3074 Fragments arranged by size: 3074 939 765 552 335 117 105 96 66 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2463 2646 2652 2713 4136 4153 Size: 1255 650 233 183 6 61 1423 17 Cuts at: 4153 4306 4491 4936 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1423 1255 650 445 233 185 183 153 61 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4355 6067 96 Size: 274 80 33 51 1443 2378 1712 138 Fragments arranged by size: 2378 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2677 2709 2875 2907 6069 6101 2677 Size: 32 166 32 3162 32 2685 Fragments arranged by size: 3162 2685 166 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2070 1316 Size: 754 5355 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3160 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 2445 4238 2445 Size: 1793 4316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI