(Circular) (Six-base) MAPSORT of: pFN21AB7729.seq Check: 3748 from: 1 to: 5998 pFN21AB7729 5998 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2203 2610 2627 4061 5445 5713 1632 Size: 495 76 407 17 1434 1384 268 1917 Fragments arranged by size: 1917 1434 1384 495 407 268 76 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 3170 3239 4897 5874 1203 Size: 1967 69 1658 977 1327 Fragments arranged by size: 1967 1658 1327 977 69 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 2152 4727 2152 Size: 2575 3423 AleI CACnn'nnGTG Cuts at: 1166 2141 1166 Size: 975 5023 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3173 1184 Size: 1989 4009 AlwNI CAG_nnn'CTG Cuts at: 2057 2605 3741 5313 2057 Size: 548 1136 1572 2742 Fragments arranged by size: 2742 1572 1136 548 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 2415 2476 2766 2996 3231 3307 3533 3685 Size: 922 61 290 230 235 76 226 152 Cuts at: 3685 5794 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 922 290 235 230 226 152 76 61 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3323 1870 Size: 73 1380 4545 Fragments arranged by size: 4545 1380 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3051 3084 3051 Size: 33 5965 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 4774 618 Size: 325 217 627 1532 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3195 3317 1391 Size: 1804 122 4072 Fragments arranged by size: 4072 1804 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 2351 2958 2967 928 Size: 971 332 120 607 9 3959 Fragments arranged by size: 3959 971 607 332 120 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BfrBI ATG'CAT Cuts at: 2834 3107 2834 Size: 273 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4609 136 Size: 107 122 71 631 605 2937 1525 Fragments arranged by size: 2937 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 4059 5215 1790 Size: 2269 1156 2573 Fragments arranged by size: 2573 2269 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2841 3729 4687 Size: 416 53 83 399 24 1424 888 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1424 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2013 4658 1239 Size: 774 2645 2579 Fragments arranged by size: 2645 2579 774 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3019 3789 493 Size: 711 1815 770 2702 Fragments arranged by size: 2702 1815 770 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2575 4431 4829 5103 5250 5661 2575 Size: 1856 398 274 147 411 2912 Fragments arranged by size: 2912 1856 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2265 2295 1178 Size: 30 165 30 862 30 4881 Fragments arranged by size: 4881 862 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2833 3203 1186 Size: 1647 370 3981 Fragments arranged by size: 3981 1647 370 BseYI C'CCAG_C Cuts at: 5201 5697 5201 Size: 496 5502 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 1163 Size: 573 706 4719 Fragments arranged by size: 4719 706 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 3195 4059 4144 5215 2385 Size: 810 864 85 1071 3168 Fragments arranged by size: 3168 1071 864 810 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 3195 3317 4059 4144 5215 1391 Size: 399 595 810 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 810 742 595 399 122 85 BspEI T'CCGG_A Cuts at: 2575 5661 2575 Size: 3086 2912 BspHI T'CATG_A Cuts at: 2191 3887 5617 2191 Size: 1696 1730 2572 Fragments arranged by size: 2572 1730 1696 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3353 844 Size: 2509 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2739 2804 2836 4493 4667 65 Size: 1183 1491 65 32 1657 174 1396 Fragments arranged by size: 1657 1491 1396 1183 174 65 32 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2469 3227 2143 Size: 326 758 4914 Fragments arranged by size: 4914 758 326 BstYI r'GATC_y Cuts at: 1972 3209 3328 3695 4080 4097 5549 5635 5647 Size: 1237 119 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1237 385 367 346 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2533 513 Size: 552 939 529 3978 Fragments arranged by size: 3978 939 552 529 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3514 4308 4328 3040 Size: 474 794 20 4710 Fragments arranged by size: 4710 794 474 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2872 2907 529 Size: 35 2308 35 3620 Fragments arranged by size: 3620 2308 35 35 DraI TTT'AAA Cuts at: 2745 3302 2745 Size: 557 5441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 3445 4334 Size: 54 1425 50 275 18 543 1072 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1072 889 543 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 3928 1353 Size: 1440 1135 3423 Fragments arranged by size: 3423 1440 1135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3193 3193 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2013 2127 2203 2610 2627 4061 4658 Size: 393 381 114 76 407 17 1434 597 Cuts at: 4658 5445 5713 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1434 787 597 407 393 381 268 114 76 17 EcoRI G'AATT_C Cuts at: 2476 3231 3307 3685 2476 Size: 755 76 378 4789 Fragments arranged by size: 4789 755 378 76 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2270 2302 3201 Size: 32 135 32 566 32 100 32 899 Cuts at: 3201 3233 3262 3294 4639 4671 4713 4745 1373 Size: 32 29 32 1345 32 42 32 2626 Fragments arranged by size: 2626 1345 899 566 135 100 42 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 2098 748 Size: 1350 4648 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 2244 3597 2244 Size: 1353 4645 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5112 5296 1305 Size: 3807 184 2007 Fragments arranged by size: 3807 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3633 Fragments arranged by size: 3633 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2369 2847 3956 4315 4474 518 Size: 648 975 228 478 1109 359 159 2042 Fragments arranged by size: 2042 1109 975 648 478 359 228 159 MspA1I CmG'CkG Cuts at: 1606 3206 3414 4091 5239 5484 1606 Size: 1600 208 677 1148 245 2120 Fragments arranged by size: 2120 1600 1148 677 245 208 NcoI C'CATG_G Cuts at: 513 1065 2004 2533 513 Size: 552 939 529 3978 Fragments arranged by size: 3978 939 552 529 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NsiI A_TGCA'T Cuts at: 2836 3109 2836 Size: 273 5725 NspI r_CATG'y Cuts at: 1132 1229 3174 3243 3356 4901 1132 Size: 97 1945 69 113 1545 2229 Fragments arranged by size: 2229 1945 1545 113 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 3170 3239 4897 3170 Size: 69 1658 4271 Fragments arranged by size: 4271 1658 69 PflMI CCAn_nnn'nTGG Cuts at: 1108 2018 2291 1108 Size: 910 273 4815 Fragments arranged by size: 4815 910 273 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PmlI CAC'GTG Cuts at: 3019 3019 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PstI C_TGCA'G Cuts at: 830 3350 830 Size: 2520 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 3206 3206 Size: 5998 SacI G_AGCT'C Cuts at: 3195 3195 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 3262 4246 5958 3262 Size: 984 1712 3302 Fragments arranged by size: 3302 1712 984 SexAI A'CCwGG_T Cuts at: 2688 2688 Size: 5998 SfcI C'TryA_G Cuts at: 826 1045 2754 3346 4481 5162 5353 826 Size: 219 1709 592 1135 681 191 1471 Fragments arranged by size: 1709 1471 1135 681 592 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4102 5003 5265 820 Size: 197 926 2159 901 262 1553 Fragments arranged by size: 2159 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2004 2533 3437 513 Size: 552 939 529 904 3074 Fragments arranged by size: 3074 939 904 552 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4025 4042 4195 4380 4825 325 Size: 1255 2445 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3260 4244 5956 Size: 274 80 33 51 1443 1283 984 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1712 1443 1283 984 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2265 2297 5958 5990 2265 Size: 32 3661 32 2273 Fragments arranged by size: 3661 2273 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 2554 3042 4127 2554 Size: 488 1085 4425 Fragments arranged by size: 4425 1085 488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI