(Circular) (Six-base) MAPSORT of: pFN21AB9980.seq Check: 5647 from: 1 to: 6829 pFN21AB9980 6829 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4172 1937 Size: 2235 4594 Acc65I G'GTAC_C Cuts at: 4150 4150 Size: 6829 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3252 4892 6276 6544 1632 Size: 1620 1640 1384 268 1917 Fragments arranged by size: 1917 1640 1620 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 2851 5728 6705 1203 Size: 1648 2877 977 1327 Fragments arranged by size: 2877 1648 1327 977 AgeI A'CCGG_T Cuts at: 5660 5660 Size: 6829 AhdI GACnn_n'nnGTC Cuts at: 5558 5558 Size: 6829 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6829 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6829 AlwNI CAG_nnn'CTG Cuts at: 3794 4572 6144 3794 Size: 778 1572 4479 Fragments arranged by size: 4479 1572 778 ApaLI G'TGCA_C Cuts at: 4886 6042 4886 Size: 1156 5673 ApoI r'AATT_y Cuts at: 1493 2130 2280 2898 3155 3383 3751 4138 4364 Size: 637 150 618 257 228 368 387 226 Cuts at: 4364 4516 6625 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 637 618 387 368 257 228 226 152 150 AseI AT'TA_AT Cuts at: 160 1032 5383 160 Size: 872 4351 1606 Fragments arranged by size: 4351 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 2231 4154 1870 Size: 73 288 1923 4545 Fragments arranged by size: 4545 1923 288 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2100 2133 2100 Size: 33 6796 BamHI G'GATC_C Cuts at: 2549 4159 4526 2549 Size: 1610 367 4852 Fragments arranged by size: 4852 1610 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4150 5605 618 Size: 325 217 627 2363 1455 1842 Fragments arranged by size: 2363 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4148 1391 Size: 2757 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3492 3969 928 Size: 971 1593 477 3788 Fragments arranged by size: 3788 1593 971 477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5020 5054 1903 Size: 34 3083 34 3678 Fragments arranged by size: 3678 3083 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5931 4721 Size: 1210 5619 BclI T'GATC_A Cuts at: 1548 2290 1548 Size: 742 6087 BfrBI ATG'CAT Cuts at: 2893 2893 Size: 6829 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5440 136 Size: 107 122 71 631 605 3768 1525 Fragments arranged by size: 3768 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3295 3593 6824 3295 Size: 298 3231 3300 Fragments arranged by size: 3300 3231 298 BlpI GC'TnA_GC Cuts at: 4246 4246 Size: 6829 Bme1580I G_kGCm'C Cuts at: 1790 4890 6046 1790 Size: 3100 1156 2573 Fragments arranged by size: 3100 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4560 5518 442 Size: 416 53 83 399 24 3143 958 1753 Fragments arranged by size: 3143 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3821 5489 1239 Size: 2582 1668 2579 Fragments arranged by size: 2582 2579 1668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2191 4954 5819 6117 2191 Size: 2763 865 298 2903 Fragments arranged by size: 2903 2763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2602 3777 5492 882 Size: 617 1103 1175 1715 2219 Fragments arranged by size: 2219 1715 1175 1103 617 BsaAI yAC'GTr Cuts at: 493 1204 4620 493 Size: 711 3416 2702 Fragments arranged by size: 3416 2702 711 BsaBI GATnn'nnATC Cuts at: 2067 4086 6823 2067 Size: 2019 2737 2073 Fragments arranged by size: 2737 2073 2019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4636 5018 5653 275 Size: 53 83 186 936 3103 382 635 1451 Fragments arranged by size: 3103 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2500 5262 5660 5934 6081 6492 2500 Size: 2762 398 274 147 411 2837 Fragments arranged by size: 2837 2762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2826 2856 1178 Size: 30 165 30 1423 30 5151 Fragments arranged by size: 5151 1423 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3413 3515 3620 3780 1186 Size: 2227 102 105 160 4235 Fragments arranged by size: 4235 2227 160 105 102 BseYI C'CCAG_C Cuts at: 6032 6528 6032 Size: 496 6333 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3356 1163 Size: 573 1620 4636 Fragments arranged by size: 4636 1620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4279 5040 6068 1540 Size: 275 186 2278 761 1028 2301 Fragments arranged by size: 2301 2278 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3589 4890 4975 6046 3589 Size: 1301 85 1071 4372 Fragments arranged by size: 4372 1301 1071 85 BsmI GAATG_Cn' Cuts at: 4340 4433 4340 Size: 93 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3589 4148 4890 4975 6046 1391 Size: 399 1799 559 742 85 1071 2174 Fragments arranged by size: 2174 1799 1071 742 559 399 85 BspEI T'CCGG_A Cuts at: 6492 6492 Size: 6829 BspHI T'CATG_A Cuts at: 2191 2389 4718 6448 2191 Size: 198 2329 1730 2572 Fragments arranged by size: 2572 2329 1730 198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 4184 844 Size: 1390 1950 3489 Fragments arranged by size: 3489 1950 1390 BsrBI CCG'CTC Cuts at: 1394 1823 4716 1394 Size: 429 2893 3507 Fragments arranged by size: 3507 2893 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5324 5498 65 Size: 1183 4076 174 1396 Fragments arranged by size: 4076 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5473 5660 1700 Size: 110 3663 187 2869 Fragments arranged by size: 3663 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 4889 5901 4889 Size: 1012 5817 BstAPI GCAn_nnn'nTGC Cuts at: 794 2420 794 Size: 1626 5203 BstBI TT'CG_AA Cuts at: 5633 5633 Size: 6829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6829 BstYI r'GATC_y Cuts at: 1972 2549 3295 3593 4159 4526 4911 4928 6380 Size: 577 746 298 566 367 385 17 1452 Cuts at: 6380 6466 6478 6824 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 746 577 566 385 367 346 298 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2324 513 Size: 552 1259 5018 Fragments arranged by size: 5018 1259 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6090 Fragments arranged by size: 6090 683 56 BtsI GCAGTG_nn' Cuts at: 3344 4345 5139 5159 3344 Size: 1001 794 20 5014 Fragments arranged by size: 5014 1001 794 20 ClaI AT'CG_AT Cuts at: 1554 3495 1554 Size: 1941 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2460 2495 529 Size: 35 1896 35 4863 Fragments arranged by size: 4863 1896 35 35 DraI TTT'AAA Cuts at: 2044 3877 4133 2044 Size: 1833 256 4740 Fragments arranged by size: 4740 1833 256 DraIII CAC_nnn'GTG Cuts at: 2420 2914 2420 Size: 494 6335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3838 5836 809 Size: 507 2522 1998 1802 Fragments arranged by size: 2522 1998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4276 5165 6794 Size: 54 1425 50 275 18 2446 889 1629 Cuts at: 6794 8 Size: 43 Fragments arranged by size: 2446 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4276 1537 Size: 275 2464 4090 Fragments arranged by size: 4090 2464 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2910 3228 3706 3718 3727 3736 3778 4120 Size: 1557 318 478 12 9 9 42 342 Cuts at: 4120 4759 1353 Size: 639 3423 Fragments arranged by size: 3423 1557 639 478 342 318 42 12 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5416 5790 5936 387 Size: 1064 138 3827 374 146 1280 Fragments arranged by size: 3827 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3252 3821 4892 5489 6276 6544 1239 Size: 393 1620 569 1071 597 787 268 1524 Fragments arranged by size: 1620 1524 1071 787 597 569 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3270 3270 Size: 6829 EcoRI G'AATT_C Cuts at: 3751 4138 4516 3751 Size: 387 378 6064 Fragments arranged by size: 6064 387 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 3050 Size: 6829 FspI TGC'GCA Cuts at: 5335 5335 Size: 6829 HaeII r_GCGC'y Cuts at: 1678 5976 1678 Size: 4298 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 3222 3254 3674 Size: 32 135 32 1040 32 578 32 420 Cuts at: 3674 3706 4049 4081 5470 5502 5544 5576 1373 Size: 32 343 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1389 1040 578 420 343 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2302 4173 4419 669 Size: 988 281 364 1871 246 3079 Fragments arranged by size: 3079 1871 988 364 281 246 HindIII A'AGCT_T Cuts at: 748 3004 748 Size: 2256 4573 HpaI GTT'AAC Cuts at: 4419 4419 Size: 6829 KpnI G_GTAC'C Cuts at: 4154 4154 Size: 6829 MfeI C'AATT_G Cuts at: 4428 4428 Size: 6829 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2786 3417 5943 6127 1305 Size: 1481 631 2526 184 2007 Fragments arranged by size: 2526 2007 1481 631 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5007 Fragments arranged by size: 5007 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2742 3474 4787 5146 5305 518 Size: 648 1576 732 1313 359 159 2042 Fragments arranged by size: 2042 1576 1313 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2782 4245 4922 6070 6315 1606 Size: 1176 1463 677 1148 245 2120 Fragments arranged by size: 2120 1463 1176 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2324 513 Size: 552 1259 5018 Fragments arranged by size: 5018 1259 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6829 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6829 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NsiI A_TGCA'T Cuts at: 2895 2895 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 2855 4187 5732 1132 Size: 97 1626 1332 1545 2229 Fragments arranged by size: 2229 1626 1545 1332 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6829 PciI A'CATG_T Cuts at: 2851 5728 2851 Size: 2877 3952 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6829 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4898 4930 6411 6443 4898 Size: 32 1481 32 5284 Fragments arranged by size: 5284 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2496 3795 1613 Size: 883 1299 4647 Fragments arranged by size: 4647 1299 883 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PspXI vC'TCGA_Gb Cuts at: 2231 2231 Size: 6829 PstI C_TGCA'G Cuts at: 830 2017 4181 830 Size: 1187 2164 3478 Fragments arranged by size: 3478 2164 1187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 PvuII CAG'CTG Cuts at: 2782 2782 Size: 6829 SalI G'TCGA_C Cuts at: 1936 4171 1936 Size: 2235 4594 SapI GCTCTTCn'nnn_ Cuts at: 3778 3778 Size: 6829 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 2011 2680 3311 5077 6789 2011 Size: 669 631 1766 1712 2051 Fragments arranged by size: 2051 1766 1712 669 631 SfcI C'TryA_G Cuts at: 826 1045 2013 3109 4177 5312 5993 6184 826 Size: 219 968 1096 1068 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 1068 968 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 4156 1872 Size: 2284 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2206 2231 4933 5834 6096 820 Size: 197 926 263 25 2702 901 262 1553 Fragments arranged by size: 2702 1553 926 901 263 262 197 25 SnaBI TAC'GTA Cuts at: 493 4620 493 Size: 4127 2702 SpeI A'CTAG_T Cuts at: 152 4103 152 Size: 3951 2878 SphI G_CATG'C Cuts at: 1132 4187 1132 Size: 3055 3774 SspI AAT'ATT Cuts at: 5 52 2049 2249 4753 5 Size: 47 1997 200 2504 2081 Fragments arranged by size: 2504 2081 1997 200 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6829 StyI C'CwwG_G Cuts at: 513 1065 2324 2424 4268 513 Size: 552 1259 100 1844 3074 Fragments arranged by size: 3074 1844 1259 552 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 2317 3356 4856 4873 5026 5211 Size: 1255 666 71 1039 1500 17 153 185 Cuts at: 5211 5656 325 Size: 445 1498 Fragments arranged by size: 1500 1498 1255 1039 666 445 185 153 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2009 2678 3309 Size: 274 80 33 51 1443 32 669 631 Cuts at: 3309 3376 5075 6787 96 Size: 67 1699 1712 138 Fragments arranged by size: 1712 1699 1443 669 631 274 138 80 67 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2796 2828 6789 6821 2796 Size: 32 3961 32 2804 Fragments arranged by size: 3961 2804 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6829 XbaI T'CTAG_A Cuts at: 3848 4165 3848 Size: 317 6512 XhoI C'TCGA_G Cuts at: 1943 2231 1943 Size: 288 6541 XmaI C'CCGG_G Cuts at: 1870 4154 1870 Size: 2284 4545 XmnI GAAnn'nnTTC Cuts at: 4958 4958 Size: 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI